... vesicles in the Drosophila larval salivary gland as a model, we followed the spatiotemporal regulation of Rho1, which in turn creates distinct organization patterns of actin and myosin. After vesicle fusion, low levels of activated Rho1 reach the vesicle membrane and drive actin nucleation in an uneven...

.... Here, we identified the conserved actin-crosslinking protein Swip-1 as a novel regulator controlling exocytosis of glue granules in the Drosophila salivary gland. Real-time imaging revealed that Swip-1 is simultaneously recruited with F-actin onto secreting granules in proximity to the apical membrane...

... sites in both yeast cells and the Drosophila larval salivary glands, and this localization is mediated by an N-terminal ER membrane anchor and conserved C-terminal sequences. The C-terminus of Hobbit binds to plasma membrane phosphatidylinositols, and the distribution of these lipids is altered...

...); after budding from the TGN, granules undergo modifications, including an increase in size. These changes occur during a poorly understood process called secretory granule maturation. Here, we leverage the Drosophila larval salivary glands as a model to characterize a novel role for Rab GTPases during...

...-CSF receptor in rodent Purkinje cells . J. Neurosci.   18 , 10481 - 10492 . Nakanishi , Y. , Sugiura , F. , Kishi , J.-I. and Hayakawa , T. ( 1986 ). Collagenase inhibitor stimulates cleft formation during early morphogenesis of mouse salivary gland . Dev. Biol.   113...

...Duy T. Tran; Kelly G. Ten Hagen ABSTRACT Real-time imaging of regulated exocytosis in secreting organs can provide unprecedented temporal and spatial detail. Here, we highlight recent advances in 3D time-lapse imaging in Drosophila salivary glands at single-granule resolution. Using fluorescently...

..., and investigated cell migration using two-color confocal time-lapse microscopy to image cell and extracellular-matrix dynamics in developing salivary glands. We labeled submandibular salivary gland (SMG) epithelial cells with green fluorescent protein and matrix with fluorescent fibronectin. Surprisingly, we...

... distribution of Ins P 3 Rs we report in salivary glands, distributed along the extended acinar lumen, requires the higher levels of receptor expression seen by Giovannucci et al. ( Giovannucci et al., 2002 ). However, alone, these lines of evidence do not directly link Ins P 3 R number and distribution...

... in the dissociated rat parotid gland. Salivary gland function is accomplished primarily by the compartmentalized epithelial domains, acini and ducts, the former involved in the production of primary saliva and the latter involved in its modification. Upon muscarinic stimulation with carbachol, both domains showed...

... immunofluorescence and immunoblot analysis for cystatin, a salivary gland acinar cell-specific protein found to be produced by differentiated HSG cells. Matrigel contains multiple extracellular matrix proteins, however, laminin-1 was identified as the major matrix component that induced HSG cell acinar development...

... salivary gland (SSG) served as model to assess the role of epidermal growth factor (EGF) receptor signaling during emergence of cell lineage diversity. Temporal fluctuations in EGF receptor mRNA levels coincident with crucial differentiative cell lineage transitions were revealed by RNase protection...

...Rebecca Kellum; Jordan W. Raff; Bruce M. Alberts ABSTRACT Heterochromatin protein 1 (HP1) was initially discovered as a protein that is associated with the heterochromatin at the chromocenter of polytene chromosomes in Drosophila larval salivary glands. In this paper we investigate the localization...

... salivary glands and compare the levels of expression between the control and IPR-induced parotid glands. Fig. 3 displays the immunoreaction pattern seen in both control and IPR-induced parotid glands when monoclonal antibody S-44 was used in the immunoblotting assay. A 48 kDa protein was detected...