...Pil Jung Kang; Rachel Mullner; Kendra Lian; Hay-Oak Park ABSTRACT Cell polarization generally occurs along a single axis that is directed by a spatial cue. Cells of the budding yeast Saccharomyces cerevisiae undergo polarized growth and oriented cell division in a spatial pattern by selecting...

... proteins of the two mating partners quickly re-equilibrate their localization resulting in a uniform LD population. Lipid droplets Endoplasmic reticulum Saccharomyces cerevisiae Protein targeting Mating Membrane fusion Seipin Triacylglycerol Steryl esters Schweizerischer...

... for the transfer of ER-resident membrane-spanning proteins to the surface of lipid droplets. Lipid droplets Endoplasmic reticulum Saccharomyces cerevisiae Perilipins Seipin Triacylglycerols Steryl esters ZonMw http://dx.doi.org/10.13039/501100001826 91217002 Aard- en...

... of proteins that sediment under stress conditions. We performed a systems-wide analysis of Saccharomyces cerevisiae by combining stable isotope labeling by amino acids in cell culture (SILAC) with cell fractionation to identify proteins that become more pelletable after acute heat shock. Differentially...

...’, when transport carriers are produced. At the late TGN, various coat/adaptor proteins exhibit distinct assembly dynamics. Trans -Golgi network Membrane traffic Cisternal maturation Super-resolution live imaging Saccharomyces cerevisiae The Golgi and the trans -Golgi network (TGN...

...Márton Megyeri; Rupali Prasad; Giora Volpert; Andrzej Sliwa-Gonzalez; A. Galih Haribowo; Auxiliadora Aguilera-Romero; Howard Riezman; Yves Barral; Anthony H. Futerman; Maya Schuldiner ABSTRACT LAG1 was the first longevity assurance gene discovered in Saccharomyces cerevisiae . The Lag1 protein...

... to adjust secretory pathway capacity according to need. Ire1, the endoplasmic reticulum (ER) stress sensor and central activator of the UPR is conserved from the budding yeast Saccharomyces cerevisiae to humans. Under ER stress conditions, Ire1 clusters into foci that enable optimal UPR activation...

... and content in response to the cell state and the environment. Except for transcriptional regulation little is known about the mechanisms used to perform this complicated feat. Focusing on the yeast Saccharomyces cerevisiae , we used complementary high-content screens to follow changes in localization of most...

... governing the stability of inner nuclear membrane (INM) proteins are not well characterized. In Saccharomyces cerevisiae , the INM Asi1–Asi3 complex, principally composed of integral membrane proteins Asi1 and Asi3, is an E3 ubiquitin ligase. In addition to its well-documented function in endoplasmic...

... Summary: A review of the current knowledge of the timeline of clathrin-mediated endocytosis in the budding yeast S . cerevisiae , including discussion of recent works that focus on how this process is regulated. Saccharomyces cerevisiae Actin Clathrin-mediated endocytosis Phospholipids...

....   6 , e1000852 . 10.1371/journal.pgen.1000852 Brachmann , C. B. , Davies , A. , Cost , G. J. , Caputo , E. , Li , J. , Hieter , P. and Boeke , J. D. ( 1998 ). Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains...

... ( park.294@osu.edu ) Competing interests The authors declare no competing or financial interests. 25 11 2014 30 3 2015 © 2015. Published by The Company of Biologists Ltd 2015 Cell polarity Budding pattern Computational modeling Saccharomyces cerevisiae GTPase...

...Barbara Knoblach; Richard A. Rachubinski ABSTRACT Eukaryotic cells replicate and partition their organelles between the mother cell and the daughter cell at cytokinesis. Polarized cells, notably the budding yeast Saccharomyces cerevisiae , are well suited for the study of organelle inheritance...

...Orit Hermesh; Christian Genz; Ido Yofe; Monika Sinzel; Doron Rapaport; Maya Schuldiner; Ralf-Peter Jansen ABSTRACT Regulation of the localization of mRNAs and local translation are universal features in eukaryotes and contribute to cellular asymmetry and differentiation. In Saccharomyces cerevisiae...

...Julie Weidner; Congwei Wang; Cristina Prescianotto-Baschong; Alejandro F. Estrada; Anne Spang ABSTRACT Numerous mRNAs are degraded in processing bodies (P bodies) in Saccharomyces cerevisiae . In logarithmically growing cells, only 0–1 P bodies per cell are detectable. However, the number...

... proteins, such as the oleosins in plants and perilipins (PLINs) in animal cells. Saccharomyces cerevisiae , however, has no homologues of these scaffolding proteins. To analyze a possible function of these proteins in the biogenesis of LDs, oleosin and perilipin family members (PLIN1, ADRP/PLIN2 and TIP47...

... of Mdm10 in ergosterol trafficking. References Altmann   K. , Westermann   B. ( 2005 ). Role of essential genes in mitochondrial morphogenesis in Saccharomyces cerevisiae.   Mol. Biol. Cell   16 , 5410 – 5417 . 10.1091/mbc.E05-07-0678 Becker   T. , Wenz   L. S. , Thornton   N...

... 2013. Published by The Company of Biologists Ltd 2013 Gβ phosphorylation Saccharomyces cerevisiae Chemotropism Polarized growth Reorientation Yeast mating response In metazoans, directed cell movement in response to a chemical gradient (chemotaxis), plays a vital role...

.... Previously we demonstrated that PKA catalytic isoforms Tpk2 and Tpk3 localise with processing and stress bodies in Saccharomyces cerevisiae . Here, we show that Tpk2 and Tpk3 are associated with translation initiation factors Pab1 and Rps3 in exponentially growing cells. Glucose starvation promotes the loss...

... proteins, including other transcription factors and signaling molecules that comprise a large proportion of the eukaryotic proteome. The Saccharomyces cerevisiae strains used in this study are listed in supplementary material Table S1 . The HSP12 - GFP fusion (RGS1) was generated by genomic...