... no competing or financial interests. © 2023. Published by The Company of Biologists Ltd 2023 Summary: A summary of how tethers play a key role in the recognition of specific cargo containers and in membrane fusion. Golgi Rab GTPase SNARE Endosomes Membrane fusion Membrane traffic...

... formed double-membrane structure called the autophagosome, and subsequently targeted for degradation in the lytic compartment of the cell. The fusion of autophagosomes with the lytic compartment is a tightly regulated step and involves membrane-bound SNARE proteins. These play a crucial role...

... gene set. An upregulated SNARE protein (NPSNB) was predominantly plasma membrane localised and enriched in the vicinity of CVs, supporting the association with this organelle found in the transcriptomic analysis. We therefore confirm that transcriptomic approaches can identify known and novel players...

...-Golgi vesicles at the rhabdomere base, and nSyb deficiency impairs rhabdomeric transport and induces accumulation of Rh1 and vesicles in the cytoplasm; this is similar to the effects of Rab11 loss. These results indicate that nSyb acts as a post-Golgi SNARE toward rhabdomeres. Surprisingly, in Rab11...

... that the original work is properly attributed. Summary: Syntaxin-4 knockout reduces insulin-stimulated glucose transport, depletes levels of cellular GLUT4 and inhibits secretion of adiponectin but only modestly affects the translocation capacity of the cells. GLUT4 SNARE Syntaxin Membrane...

... growth depends on non-fusogenic Sec22b–Stx1 SNARE complexes at endoplasmic reticulum (ER)–PM contacts. Here, we show that Sec22b interacts with members of the extended synaptotagmin (E-Syt) family of ER lipid transfer proteins (LTPs), and this interaction depends on the longin domain of Sec22b...

... network. Here, we examine its role in the sorting of VAMP7 (also known as TI-VAMP) – an R-SNARE possessing a regulatory longin domain (LD) – into rhodopsin transport carriers (RTCs). During RTC formation and trafficking, VAMP7 colocalizes with the ciliary cargo rhodopsin and interacts with the Rab11...

...Khamal K. Ampah; Jennifer Greaves; Amber S. Shun-Shion; Asral W. Asnawi; Jessica A. Lidster; Luke H. Chamberlain; Mark O. Collins; Andrew A. Peden ABSTRACT STX19 is an unusual Q a -SNARE as it lacks a C-terminal transmembrane domain. However, it is efficiently targeted to post-Golgi membranes. Here...

...Kin Pan Chung; Yonglun Zeng; Yimin Li; Changyang Ji; Yiji Xia; Liwen Jiang; Jenny Russinova ABSTRACT Soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptors (SNAREs) are well-known for their role in controlling membrane fusion, the final, but crucial step, in vesicular...

... trafficking. To understand the evolutionary history of trypanosomatid trafficking, where multiple lifestyles and mechanisms of host interactions are known, we examined protein families central to defining intracellular compartments and mediating transport, namely Rabs, SNAREs and RabGAPs, across all available...

... oxidatively damaged NOX2. We identified syntaxin-7, SNAP23 and VAMP8 as the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins mediating this process. Our data describe a key mechanism of how dendritic cells sustain ROS production after antigen uptake that is required...

...Frédéric Daste; Thierry Galli; David Tareste ABSTRACT Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins constitute the core membrane fusion machinery of intracellular transport and intercellular communication. A little more than ten years ago, it was proposed...

... that are dependent on clathrin and those that are not. The SNARE proteins VAMP2, VAMP3 and VAMP8 are internalized in a clathrin-dependent manner. However, the full scope of their endocytic behavior has not yet been elucidated. Here, we found that VAMP2, VAMP3 and VAMP8 are localized on plasma membrane invaginations...

[email protected] ) Competing interests The authors declare no competing or financial interests. 4 11 2014 16 3 2015 © 2015. Published by The Company of Biologists Ltd 2015 Exocytosis Mast cell Membrane trafficking Munc18 Secretion SNARE Munc18-2 (also known as STXBP2...

... (SNAREs). Although SNARE complexes are fairly promiscuous in vitro , substantial specificity is achieved in cells owing to the spatial segregation and shielding of SNARE motifs prior to association with cognate Q-SNAREs. In this study, we identified phosphatidylinositol 4-kinase IIα (PI4K2A) as a binding...

... by syntaxin13 (officially known as STX12) and SNAP23; a similar dependence on these two SNARE proteins was observed for invadopodium-based matrix degradation and cell invasion. Inhibition of SNARE function impaired the delivery of Src and EGFR to developing invadopodia, as well as the β1-integrin-dependent...

...Aram Megighian; Mauro Zordan; Sergio Pantano; Michele Scorzeto; Michela Rigoni; Damiano Zanini; Ornella Rossetto; Cesare Montecucco Summary The SNARE proteins VAMP/synaptobrevin, SNAP-25 and syntaxin are core components of the apparatus that mediates neurotransmitter release. They form...

... attachment protein receptor (SNARE) protein assembly. To monitor dynamic changes in syntaxin 1A (Syx) conformation along exocytosis, we constructed a novel fluorescent Syx-based probe that can be efficiently incorporated within endogenous SNARE complexes, support exocytosis, and report shifts in Syx between...

...Gayoung Anna Han; Na-Ryum Bin; Soo-Young Ann Kang; Liping Han; Shuzo Sugita Summary Munc18-1 is believed to prime or stimulate SNARE-mediated membrane fusion/exocytosis through binding to the SNARE complex, in addition to chaperoning its cognate syntaxins. Nevertheless, a Munc18-1 mutant...

...Orly Laufman; WanJin Hong; Sima Lev Summary Multisubunit tethering complexes (MTCs) positively regulate vesicular fusion by as yet unclear mechanism. In this study we provide evidence that the MTC COG enhances the assembly of fusogenic Golgi SNARE complexes and concomitantly prevents nonfusogenic...