.... Endoplasmic reticulum Replicative Brucella -containing vacuole rBCV FIB/SEM tomography Correlative light and electron microscopy CLEM Sec61 Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung 10.13039/501100001711 31003A_173119 European Research Council...

... of mycolactone, which targets the Sec61 complex and inhibits the co-translational translocation of secretory proteins into the endoplasmic reticulum. In this study, we investigate the effect of mycolactone on the Sec61-dependent biogenesis of different classes of transmembrane protein (TMP). Our data suggest...

...Tina Junne; Martin Spiess ABSTRACT The Sec61 translocon catalyzes translocation of proteins into the endoplasmic reticulum and the lateral integration of transmembrane segments into the lipid bilayer. Integration is mediated by the hydrophobicity of a polypeptide segment consistent...

...Michael McKenna; Rachel E. Simmonds; Stephen High ABSTRACT The virulence factor mycolactone is responsible for the immunosuppression and tissue necrosis that characterise Buruli ulcer, a disease caused by infection with Mycobacterium ulcerans . In this study, we confirm that Sec61, the protein...

... Schuierer; Frank Petersen; John A. Tallarico; Tom A. Rapoport; Martin Spiess; Dominic Hoepfner ABSTRACT A new cyclic decadepsipeptide was isolated from Chaetosphaeria tulasneorum with potent bioactivity on mammalian and yeast cells. Chemogenomic profiling in S. cerevisiae indicated that the Sec61 translocon...

...-2765(03)00304-6 Meyer   H. A. , Grau   H. , Kraft   R. , Kostka   S. , Prehn   S. , Kalies   K. U. , Hartmann   E. ( 2000 ). Mammalian Sec61 is associated with Sec62 and Sec63.   J. Biol. Chem.   275 , 14550 – 14557 . 10.1074/jbc.275.19.14550 Mothes   W. , Prehn...

...Benedict C. S. Cross; Stephen High The membrane integration of polytopic proteins is coordinated at the endoplasmic reticulum (ER) by the conserved Sec61 translocon, which facilitates the lateral release of transmembrane (TM) segments into the lipid phase during polypeptide translocation. Here we...

... suggests that the protein-conducting channel formed by the Sec61 complex is responsible for both forward and retrograde transport of proteins across the ER membrane. For transmembrane proteins, retrograde transport can be viewed as a reversal of integration of membrane proteins into the ER membrane...

...Jon Broughton; Dominique Swennen; Barrie M. Wilkinson; Philippe Joyet; Claude Gaillardin; Colin J. Stirling ABSTRACT The Sec61 protein is required for protein translocation across the ER membrane in both yeast and mammals and is found in close association with polypeptides during their membrane...