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Keywords: SBF-SEM
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Journal Articles
Brain endothelial tricellular junctions as novel sites for T cell diapedesis across the blood–brain barrier
Open AccessIn collection:
Cell migration
Mariana Castro Dias, Adolfo Odriozola Quesada, Sasha Soldati, Fabio Bösch, Isabelle Gruber, Tobias Hildbrand, Derya Sönmez, Tejas Khire, Guillaume Witz, James L. McGrath, Jörg Piontek, Masuo Kondoh, Urban Deutsch, Benoît Zuber, Britta Engelhardt
Journal:
Journal of Cell Science
J Cell Sci (2021) 134 (8): jcs253880.
Published: 26 April 2021
... physiological flow with serial block-face scanning electron microscopy (SBF-SEM), we have identified BBB tricellular junctions as novel sites for T cell diapedesis across the BBB. Downregulated expression of tricellular junctional proteins or protein-based targeting of their interactions in pMBMEC monolayers...
Includes: Supplementary data
Journal Articles
Patterns of organelle ontogeny through a cell cycle revealed by whole-cell reconstructions using 3D electron microscopy
Available to PurchaseIn collection:
Imaging
Journal:
Journal of Cell Science
J Cell Sci (2017) 130 (3): 637–647.
Published: 1 February 2017
... spatiotemporal re-arrangements to ensure correct distribution to daughter cells. Here, serial block face scanning electron microscopy (SBF-SEM) was used to reconstruct whole individual cells at different stages of the cell cycle to give an unprecedented temporal, spatial and quantitative view of organelle...
Includes: Supplementary data
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2013) 126 (24): 5748–5757.
Published: 15 December 2013
... form of the parasite. We show here, using new imaging techniques including serial block-face scanning electron microscopy (SBF-SEM), that the distal tip of the new flagellum in the bloodstream form is embedded within an invagination in the cell body plasma membrane, named the groove. We suggest...
Includes: Supplementary data