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Keywords: S. pombe
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Journal Articles
J Cell Sci (2021) 134 (4): jcs253518.
Published: 22 February 2021
... URA3 gene for selection in S. pombe ura4 mutants ( Lin and Smith, 1995 ). Plasmids were extracted from four to eight separate E. coli transformants for each mutant and sequenced. Plasmids with the desired mutations were introduced into S. pombe strain GP9806 ( h 90 rec10-175::kanMX6 ura4-294...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (10): jcs238709.
Published: 22 May 2020
... pombe Rdh54 (TID1) acts with Rhp54 (RAD54) to repair meiotic double-strand breaks . Mol. Biol. Cell   14 , 4707 - 4720 . 10.1091/mbc.e03-05-0288 Cervantes , M. D. , Farah , J. A. and Smith , G. R. ( 2000 ). Meiotic DNA breaks associated with recombination in S. pombe . Mol...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (11): jcs230706.
Published: 31 May 2019
... on chemoattractant gradient detection in yeast, which occurs at a motile polarity patch. It further describes the stabilization and hyperpolarization process underlying cell fusion. Cell fusion Cell polarity Chemotropism Pheromone gradient Cdc42 MAPK S. cerevisiae S. pombe All cells...
Journal Articles
J Cell Sci (2018) 131 (16): jcs216895.
Published: 23 August 2018
.../user-licence-1-1/ Summary: Cytokinetic contractile rings assemble normally in fission yeast without the signaling cascade called the septation initiation network, but the rings are unstable, so cytokinesis fails and cells often lyse. Cytokinesis S. pombe SIN Cell division...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (22): 4057–4062.
Published: 15 November 2015
... to release membrane invaginations and to facilitate surface area expansion. Yeast Eisosome Plasma membrane Protoplast Microfluidics S. pombe The plasma membrane forms a continuous barrier between a cell and its environment. During mechanical and hypoosmotic stress, the surface area...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (13): 2259–2268.
Published: 01 July 2015
.... and Pollard , T. D. ( 2014 ). Contractile ring stability in S. pombe depends on F-BAR protein Cdc15p and Bgs1p transport from the Golgi complex . Cell Rep.   8 , 1533 - 1544 . 10.1016/j.celrep.2014.07.048 Bahler , J. , Wu , J.-Q. , Longtine , M. S. , Shah , N. G. , McKenzie...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (4): 741–754.
Published: 15 February 2015
... by The Company of Biologists Ltd 2015 S. pombe Cytokinesis SIN Asymmetric events are of fundamental importance in biology. Asymmetry of centrosome behaviour and inheritance is also implicated in an increasing number of cellular and developmental processes ( Knoblich, 2010 ; Tajbakhsh et al...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (2): 266–280.
Published: 15 January 2015
... with ribosomes ( Zinzalla et al., 2011 ), raise the possibility that, in fission yeast, the TORC2 complex might be also associated with ribosomes, providing a suitable platform to regulate Pck2 translation and MAPK activation in response to environmental changes. S. pombe strains used in this work...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (14): 3149–3161.
Published: 15 July 2014
....   EMBO J.   13 , 3011 – 3019 . Fankhauser   C. , Marks   J. , Reymond   A. , Simanis   V. ( 1993 ). The S. pombe cdc16 gene is required both for maintenance of p34cdc2 kinase activity and regulation of septum formation: a link between mitosis and cytokinesis?   EMBO J.   12 , 2697...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (11): 2460–2470.
Published: 01 June 2014
... 14 02 2014 © 2014. Published by The Company of Biologists Ltd 2014 Genome stability Ribonucleotide reductase CRL4 Cdt2 ubiquitin ligase Intrinsically disordered proteins S. pombe Cancer model Correct regulation of the levels of the building blocks of DNA is emerging...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (6): 1346–1356.
Published: 15 March 2014
... ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. HeLa S. pombe TOR Torin1 Wee1 Cells regulate growth, metabolism and proliferation through target of rapamycin (TOR) kinase signalling. Fission yeast...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (21): 5052–5061.
Published: 01 November 2013
... the kinase domain markedly increased the sensitivities of the analogue-sensitive kinases to ATP analogues in three out of five S. pombe kinases (i.e. Plo1, Orb5 and Wee1) that harbor this conserved methionine residue. Kinome alignment established that a methionine residue is found at this site in 5–9...
Includes: Supplementary data
Journal Articles
J Cell Sci (2012) 125 (24): 5955–5959.
Published: 15 December 2012
... 2012 S. pombe TOR Rapamycin Gcn2 eIF2α In response to diverse environmental stresses, the phosphorylation of eukaryotic initiation factor-2 (eIF2α) induces a programme of gene expression that mitigates cellular injury. Several protein kinases phosphorylate eIF2α at serine 51, leading...
Includes: Supplementary data
Journal Articles
J Cell Sci (2012) 125 (11): 2655–2664.
Published: 01 June 2012
.... Activation of human AMPK depends on a marked decrease in intracellular ATP levels and phosphorylation by the upstream kinase LKB1 at Thr172 (Thr189 in S. pombe ), located in the activation loop of the catalytic α-subunit. This phosphorylation is quantitatively more important (100-fold) than the allosteric...
Includes: Supplementary data
Journal Articles
J Cell Sci (2012) 125 (8): 1920–1928.
Published: 15 April 2012
... et al., 2000 ). To check whether this was also the case in S. pombe , we used FM4-64 to stain vacuolar membranes ( Fig. 5D ). If the vacuolar fusion process diminishes, staining with FM4-64 remains visible only as small dots in the cytoplasm because the endosomes are unable to fuse; however...
Includes: Supplementary data
Journal Articles
J Cell Sci (2009) 122 (9): 1342–1351.
Published: 01 May 2009
... distinct propagation characteristics, and are therefore different. To further characterize the Cin cif1 cells, the sensitivity to hygromycin B was examined by spotting the different strains on media containing this antibiotic. Hygromycin B lowers the viability of S. pombe cells with defects...
Includes: Supplementary data
Journal Articles
J Cell Sci (2009) 122 (8): 1163–1173.
Published: 15 April 2009
... for overexpression of CLN3, which might have distorted its subcellular distribution. Nevertheless, a recent study ( Storch et al., 2007 ) concluded that, like Btn1p in S. pombe , trafficking of wild-type CLN3 to lysosomes was considerably slower than lysosome membrane-resident proteins, and that CLN3 remains...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2008) 121 (24): 4055–4068.
Published: 15 December 2008
... its control of tip growth following heat or gravitational stresses. * Author for correspondence (e-mail: ihagan@picr.man.ac.uk ) 3 9 2008 © The Company of Biologists Limited 2008 2008 S. pombe Stress response Sty1 Pmk1 Ssp1 Tea1 Eukaryotic cells adapt...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2008) 121 (12): 1973–1980.
Published: 15 June 2008
... is reduced. * Author for correspondence (e-mail: rroddag@upo.es ) 31 3 2008 © The Company of Biologists Limited 2008 2008 Microtubule Mitosis Mitotic spindle S. pombe Spindle pole body (SPB) Spindle alignment References Baena-Lopez, L. A., Baonza, A. and Garcia...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2008) 121 (5): 601–608.
Published: 01 March 2008
... in phosphorylation. Cytokinesis S. pombe GTPase activating protein (GAP) Proteolysis * Author for correspondence (e-mail: viesturs.simanis@isrec.ch ) 19 11 2007 © The Company of Biologists Limited 2008 2008 The model proposed here imposes certain constraints upon assembly...
Includes: Supplementary data