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Keywords: S. pombe
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Journal Articles
J Cell Sci (2022) 135 (24): jcs260240.
Published: 16 December 2022
..., contrasted with the relative ease of endogenous tagging and live-cell fluorescence microscopy. To facilitate super-resolution microscopy in yeasts, we have extended the ultrastructure expansion microscopy (U-ExM) method to both S. cerevisiae and S. pombe , enabling a 4-fold isotropic expansion. We...
Includes: Supplementary data
Journal Articles
J Cell Sci (2021) 134 (4): jcs253518.
Published: 22 February 2021
.... Meiotic recombination Linear element proteins Nuclear localization signal S. pombe DSB hotspot determinants Synaptonemal complex proteins In sexually reproducing species, one generation gives rise to the next by formation of haploid gametes from diploid somatic cells; fusion of two gametes...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (10): jcs238709.
Published: 22 May 2020
... pombe Rdh54 (TID1) acts with Rhp54 (RAD54) to repair meiotic double-strand breaks . Mol. Biol. Cell   14 , 4707 - 4720 . 10.1091/mbc.e03-05-0288 Cervantes , M. D. , Farah , J. A. and Smith , G. R. ( 2000 ). Meiotic DNA breaks associated with recombination in S. pombe . Mol...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (11): jcs230706.
Published: 31 May 2019
... on chemoattractant gradient detection in yeast, which occurs at a motile polarity patch. It further describes the stabilization and hyperpolarization process underlying cell fusion. Cell fusion Cell polarity Chemotropism Pheromone gradient Cdc42 MAPK S. cerevisiae S. pombe All cells...
Journal Articles
J Cell Sci (2018) 131 (16): jcs216895.
Published: 23 August 2018
.../user-licence-1-1/ Summary: Cytokinetic contractile rings assemble normally in fission yeast without the signaling cascade called the septation initiation network, but the rings are unstable, so cytokinesis fails and cells often lyse. Cytokinesis S. pombe SIN Cell division...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (22): 4057–4062.
Published: 15 November 2015
... Article: During membrane stretching in yeast protoplasts, eisosomes disassemble to release membrane invaginations and to facilitate surface area expansion. Yeast Eisosome Plasma membrane Protoplast Microfluidics S. pombe The plasma membrane forms a continuous barrier between a cell...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (13): 2259–2268.
Published: 1 July 2015
...-BAR protein Rga7p is required for the timely delivery of a β-glucan synthetase (Bgs4p) to the cleavage furrow during cytokinesis of fission yeast. Bgs4 Cell wall Cytokinesis F-BAR proteins Golgi complex S. pombe Eukaryotic cells divide by assembling and constricting a contractile...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (4): 741–754.
Published: 15 February 2015
... by The Company of Biologists Ltd 2015 S. pombe Cytokinesis SIN Asymmetric events are of fundamental importance in biology. Asymmetry of centrosome behaviour and inheritance is also implicated in an increasing number of cellular and developmental processes ( Knoblich, 2010 ; Tajbakhsh et al...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (2): 266–280.
Published: 15 January 2015
... cells expressing the hypomorphic Rho1 allele rho1-596 ( supplementary material Fig. S2A ) ( Viana et al., 2013 ). Taken together, these results indicate that, in S. pombe , the concomitant phosphorylation of the activation domain (at T842, T846 and T984) and Rho1/Rho2 stabilization increase Pck2...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (14): 3149–3161.
Published: 15 July 2014
.... , Reymond   A. , Simanis   V. ( 1993 ). The S. pombe cdc16 gene is required both for maintenance of p34cdc2 kinase activity and regulation of septum formation: a link between mitosis and cytokinesis?   EMBO J.   12 , 2697 – 2704 . Feoktistova   A. , Morrell-Falvey   J. , Chen   J. S...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (11): 2460–2470.
Published: 1 June 2014
.... (A) Comparison of amino acid sequences of Spd1 and Spd2 orthologs of the four Schizosaccharomyces species S. pombe (Spo), S. cryophilus (Scr), S. octosporus (Soc) and S. japonicus (Sja). Residues are highlighted in black and grey when they are identical and similar, respectively, between Spd1 and Spd2...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (6): 1346–1356.
Published: 15 March 2014
... provided that the original work is properly attributed. HeLa S. pombe TOR Torin1 Wee1 Cells regulate growth, metabolism and proliferation through target of rapamycin (TOR) kinase signalling. Fission yeast Schizosaccharomyces pombe contains two TOR kinases: the non-essential Tor1...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (21): 5052–5061.
Published: 1 November 2013
... the kinase domain markedly increased the sensitivities of the analogue-sensitive kinases to ATP analogues in three out of five S. pombe kinases (i.e. Plo1, Orb5 and Wee1) that harbor this conserved methionine residue. Kinome alignment established that a methionine residue is found at this site in 5–9...
Includes: Supplementary data
Journal Articles
J Cell Sci (2012) 125 (24): 5955–5959.
Published: 15 December 2012
..., induces Gcn2-dependent phosphorylation of eIF2α. * Author for correspondence ( smo@usal.es ) 11 10 2012 © 2012. Published by The Company of Biologists Ltd 2012 S. pombe TOR Rapamycin Gcn2 eIF2α In response to diverse environmental stresses, the phosphorylation...
Includes: Supplementary data
Journal Articles
J Cell Sci (2012) 125 (11): 2655–2664.
Published: 1 June 2012
... the redistribution of AMPK from the cytoplasm to the nucleus. This response is important to advance cells into mitosis and establish a correct pre-Start G1 cell cycle arrest. All S. pombe strains used in this study are listed in supplementary material Table S3 . Standard methods were used for growth...
Includes: Supplementary data
Journal Articles
J Cell Sci (2012) 125 (8): 1920–1928.
Published: 15 April 2012
... data demonstrate the existence of an evolutionarily conserved pathway with the Vam6 and Gtr1–Gtr2 pathway activating TORC1, which in turns stimulates cell growth and inhibits sexual differentiation. * Author for correspondence ( smo@usal.es ) 12 12 2011 © 2012. 2012 S. pombe...
Includes: Supplementary data
Journal Articles
J Cell Sci (2009) 122 (9): 1342–1351.
Published: 1 May 2009
... of Cif1p is required to induce the Cin cif1 state, thus suggesting an unexpected interaction between the vital cellular role of calnexin and a function of the nucleolus. S. pombe strains used in this study are listed in Table 1 . Yeast cells were cultured in Edinburgh minimal medium (MM...
Includes: Supplementary data
Journal Articles
J Cell Sci (2009) 122 (8): 1163–1173.
Published: 15 April 2009
.... The morphology of S. pombe Golgi resemble those of mammalian cells ( Chappell and Warren, 1989 ). In wild-type cells, the multiple Golgi were easily recognisable as electron-dense ribbons or flattened cisternae of length ∼0.5-0.8 μm, often with a globular (or beaded) appearance ( Fig. 6A,B ). Most had one, two...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2008) 121 (24): 4055–4068.
Published: 15 December 2008
... its control of tip growth following heat or gravitational stresses. * Author for correspondence (e-mail: ihagan@picr.man.ac.uk ) 3 9 2008 © The Company of Biologists Limited 2008 2008 S. pombe Stress response Sty1 Pmk1 Ssp1 Tea1 Eukaryotic cells adapt...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2008) 121 (12): 1973–1980.
Published: 15 June 2008
... is reduced. * Author for correspondence (e-mail: rroddag@upo.es ) 31 3 2008 © The Company of Biologists Limited 2008 2008 Microtubule Mitosis Mitotic spindle S. pombe Spindle pole body (SPB) Spindle alignment References Baena-Lopez, L. A., Baonza, A. and Garcia...
Includes: Multimedia, Supplementary data