..., contrasted with the relative ease of endogenous tagging and live-cell fluorescence microscopy. To facilitate super-resolution microscopy in yeasts, we have extended the ultrastructure expansion microscopy (U-ExM) method to both S. cerevisiae and S. pombe , enabling a 4-fold isotropic expansion. We...

... organelles are critical regulators of cellular function. Here we identify the highly conserved Hob proteins as novel lipid-binding proteins at ER–PM contact sites. Hobbit ER–PM contact sites Phosphatidylinositol S. cerevisiae Drosophila Salivary gland Regulated exocytosis National...

.... In S. cerevisiae , Cdk1 phosphorylates securin (Pds1), which facilitates Pds1 binding and inhibits separase (Esp1). During anaphase, Esp1 cleaves the cohesin subunit Scc1 and promotes spindle elongation. Here, we show that PP2A Cdc55 directly dephosphorylates Pds1 both in vivo and in vitro . Pds1...

... on chemoattractant gradient detection in yeast, which occurs at a motile polarity patch. It further describes the stabilization and hyperpolarization process underlying cell fusion. Cell fusion Cell polarity Chemotropism Pheromone gradient Cdc42 MAPK S. cerevisiae S. pombe Schweizerischer...

..., organ or body. The physiology of ex vivo cell culture models are consequently questionable ( O'Farrell, 2011 ; Rumman et al., 2015 ; Coller et al., 2006 ). By contrast, S. cerevisiae , as a single-cell eukaryote, has been instrumental for studying quiescence. Margaret Werner-Washburne pioneered...

.... Previous studies established that homologs of the oxysterol-binding protein (OSBP) in S. cerevisiae , which comprise the Osh protein family, are necessary for efficient polarized exocytosis by supporting a late post-Golgi step. We define this step as the docking of a specific sub-population of exocytic...

... and show that S. cerevisiae Bre1p protects yeast cells from H 2 O 2 -induced cell death, whereas deletion of BRE1 enhances cell death and leads to decreased lifespan during chronological ageing. Also, we show that Bre1p activity in yeast apoptosis requires its E3 ubiquitin ligase activity, thereby...

... Ubiquitin S. cerevisiae CUE1 UBC7 Endoplasmic reticulum (ER)-associated degradation (ERAD) represents the primary means by which proteins that are misfolded, unassembled or whose levels require regulation are targeted for degradation from the secretory pathway. ERAD is a highly complex...

... are temporally regulated during cell cycle progression in S. cerevisiae . Rot1 is specifically involved in the apical-isotropic growth switch and the polarization to the neck region at the end of cell division. Conversely, it is not required for growth polarization during bud emergence or for the function...

... of Biologists Limited 2007 2007 Chitin Endocytic turnover S. cerevisiae In S. cerevisiae , chitin synthesis occurs in a highly organized fashion. It begins during the initial steps of budding, forming a growing ring that will remain at the mother side after cell division. This ring...

...Viesturs Simanis The mitotic exit network (MEN) and the septation initiation network (SIN) control events at the end of mitosis in S. cerevisiae and S. pombe , respectively. SIN initiates contraction of the actin ring and synthesis of the division septum, thereby bringing about cytokinesis. The MEN...

... cerevisiae and Xenopus oocytes under conditions that deplete nuclear RanGTP and prevent RNA export to the cytoplasm. Our data suggest that depletion of nuclear RanGTP does not significantly alter the nucleolar localization of U3 snoRNA in tsBN2 cells. Complementary studies in the budding yeast S. cerevisiae...

... ) © The Company of Biologists Limited 2001 2001 Scaffold MAP kinase Signal transduction RING-H2 domain S. cerevisiae Mating All eukaryotic cells use mitogen-activated protein kinase (MAPK) cascades as central cores of complex signal transduction pathways that respond to a wide...

... cerevisiae contains an open reading frame ( CLU1 ) that encodes a protein that is 27% identical, 50% similar, to this Dictyostelium protein. Deletion of CLU1 from S. cerevisiae did not affect cell viability, growth properties, sporulation efficiency, or frequency of occurrence of cells lacking functional...

..., Switzerland 21 09 1993 22 12 1993 © 1994 by Company of Biologists 1994 mRNA stability S. cerevisiae localization Eucaryotic gene expression depends on a large number of events that follow polymerase II transcription, but which occur before mRNA translation. The mRNA...

... University, 1-24-2, Nishishinjuku, Shinjuku-ku, Tokyo, 163-91, Japan 09 01 1989 23 06 1989 © 1989 by Company of Biologists 1989 S. cerevisiae α factor three-dimensional reconstruction cell surface growth membrane vesicle Mechanisms controlling cell surface growth...

.... Considering the ubiquitous presence of MPF, it is reasonable to suggest that yeast may have a factor similar to MPF, which regulates M phase during the yeast cell cycle. Indeed, Weintraub et al . (1982) have shown that MPF-like activity is present in extracts of some cdc mutants of S. cerevisiae...