... of Biologists Ltd 2013 Centromere Cid Cenp-A Cenp-C Cal1 Cell cycle Mitosis S phase Drosophila Accurate chromosome segregation during mitotic and meiotic divisions depends on functional centromeres. Chromosomes lacking centromere function fail to assemble kinetochores and cannot...

...Paloma García; Jon Frampton The cell-cycle-regulated Myb-family transcription factor B-Myb is crucial during S phase in many diploid cell types. We have examined the expression and function of B-Myb in megakaryocytic differentiation, during which cells progress from a diploid to a polyploid state...

... cycle stasis at the onset of S phase upon release from prolonged arrest with the inhibitors of DNA replication, hydroxyurea or aphidicolin. During arrest typical S phase markers remain present, and G0/G1 inhibitory signals such as p21 WAF1 and p27 are absent. Cell cycle stasis occurs in T-antigen...

... Biologicals). Note the strong staining only in the mitotic cell at 0 hours. Ac-H3, acetylated (Lys9,14); P-H3, phosphorylated (Ser10). Fig. 4. Exogenous CHMP1 blocks cell-cycle progression in late S-phase. IND-CHMP1 cells were induced with 10 μM muristerone for the indicated times. (A) Cells were...

... of recombinant Cdc6 in mammalian cell extracts under in vitro replication conditions. We find that Cdc6 is imported into the nucleus in G 1 phase, where it binds to chromatin and remains relatively stable. In S phase, exogenous Cdc6 is destroyed in a process that requires import into the nucleus...

...-fluorophenylamino)-7-methoxy-6-(3-[1-pyrolidino])-propoxyquinazoline, EGFR-TKI), we show that signalling through EGF-R is not required for nafenopin-induced DNA synthesis. The EGFR-TKI also prevented progression into S phase upon stimulation with TNFα, but DNA synthesis was not reduced to control levels, indicating...

... is a phosphoprotein which is hypermodified in mitosis and is rapidly converted to a faster migrating isoform as cells proceed into G 1 in preparation for S-phase. * Author for correspondence (e-mail: [email protected] ) © 1999 by Company of Biologists 1999 Schizosaccharomyces pombe DNA...

... and chicken embryonic fibroblasts. In a synchronized culture of MSB-1 cells, the immunore-activity of nuclei with 2H3, but not with 3B7, antibody decreased markedly during the progression of S phase, but returned to the normal level at the next G 1 phase. When cells were treated with Triton X-100 prior...

... in cycling cells and peaks during S phase. We report here that forced expression of the RanBPI gene disrupts the orderly execution of the cell division cycle at several stages, causing inhibition of DNA replication, defective mitotic exit and failure of chromatin decondensation during the telophase...

.... The bound population is detected throughout the nucleus in late G 1 and early S, and at discrete subnuclear sites following further progression of S-phase. We use high resolution confocal microscopy to determine the subnuclear sites of chromatin-bound Mcm proteins in comparison to the sites of replicating...

.... To test whether assembly of the replication initiation complex is an important element in the checkpoint control pathway we have investigated if DNA polymerase alpha (poll) , a component of the initiation complex, is essential for the S-phase checkpoint control. We show that germinating S. pombe spores...

...Akira Nomura ABSTRACT Previous studies ( Nomura et al. (1991) Dev. Biol . 143, 289-296 (1993) Dev. Biol . 159, 288-297) determined the time of DNA replication period (S phase) in starfish eggs fertilized either during or after oocyte maturation. Here proliferating cell nuclear antigen (PCNA...

... reduction in the phospholipids detectable inside the nucleus in all steps of the S phase. This could depend on an increased nuclear phospholipid hydrolysis, whose products should in turn activate some of the enzymes involved in the control of DNA replication. * Author for correspondence 21 09...

...James M. Roberts; Gennaro D’urso ABSTRACT Cell-free replication of SV40 DNA in extracts prepared from S phase cells is at least 20-fold more efficient than in extracts from G 1 cells. The increased activity of S phase extracts correlates with the presence of an S phase-specific cellular factor...