.../endoplasmic reticulum Ca 2+ -ATPase (SERCA) pump and of the ryanodine receptor leak were too modest to explain the large changes seen in [Ca 2+ ] SR . Instead, these changes are compatible with increased leakiness through the ryanodine receptor as the main determinant of the [Ca 2+ ] SR decline in aging...

... similar analyses of ryanodine receptors, have contributed to understanding IP 3 R behaviour. Analyses of permeabilized cells have demonstrated that the ER is the major intracellular Ca 2+ store, and that IP 3 stimulates Ca 2+ release from this store. Radioligand binding confirmed that the 4,5-phosphates...

...Paulina J. Stanczyk; Monika Seidel; Judith White; Cedric Viero; Christopher H. George; Spyros Zissimopoulos; F. Anthony Lai ABSTRACT The cardiac muscle ryanodine receptor-Ca 2+ release channel (RyR2) constitutes the sarcoplasmic reticulum (SR) Ca 2+ efflux mechanism that initiates myocyte...

...Spencer J. Richardson; Gregory A. Steele; Esther M. Gallant; Alexander Lam; Charles E. Schwartz; Philip G. Board; Marco G. Casarotto; Nicole A. Beard; Angela F. Dulhunty ABSTRACT Ryanodine receptor (RyR) Ca 2+ channels are central to striated muscle function and influence signalling in neurons...

...-molecule localisation microscopy showed that junctions in animals producing above-normal levels of junctophilin-2 were enlarged, allowing the re-organisation of the primary functional protein within it, the ryanodine receptor (RyR; in this paper, we use RyR to refer to the myocardial isoform RyR2...

...Ahmed Handhle; Chloe E. Ormonde; N. Lowri Thomas; Catherine Bralesford; Alan J. Williams; F. Anthony Lai; Spyros Zissimopoulos ABSTRACT Cardiac muscle contraction requires sarcoplasmic reticulum (SR) Ca 2+ release mediated by the quaternary complex comprising the ryanodine receptor 2 (RyR2...

... of the sarcoplasmic reticulum (SR) Ca 2+ store where it modifies Ca 2+ signalling in the heart and skeletal muscle through its regulation of ryanodine receptor (RyR) Ca 2+ release channels. Junctin is required for normal muscle function as its knockout leads to abnormal Ca 2+ signalling, muscle dysfunction...

... similarity with a conserved sequence present in all three ryanodine receptor (RyR) isoforms. We now report that Bcl-2 co-immunoprecipitated with RyRs in ectopic expression systems and in native rat hippocampi, indicating that endogenous RyR–Bcl-2 complexes exist. Purified RyR domains containing the putative...

...Mirko Vukcevic; Francesco Zorzato; Simone Keck; Dimitrios A. Tsakiris; Jennifer Keiser; Rick M. Maizels; Susan Treves Summary Mutations in RYR1 , the gene encoding ryanodine receptor 1, are linked to a variety of neuromuscular disorders including malignant hyperthermia (MH), a pharmacogenetic...

... membranes MAM Ryanodine receptor Uniporter Ca 2+ signalling reactive oxygen species ROS Isolated mitochondria are competent to produce energy on their own and are not particularly responsive to changes in their environment that mimic intracellular signals. These observations supported...

...Spyros Zissimopoulos; Sara Seifan; Chloe Maxwell; Alan J. Williams; F. Anthony Lai The FK506-binding proteins (FKBP12 and FKBP12.6; also known as FKBP1A and FKBP1B, respectively) are accessory subunits of the ryanodine receptor (RyR) Ca 2+ release channel. Aberrant RyR2–FKBP12.6 interactions have...

...Meredith N. Schulson; David R. L. Scriven; Patrick Fletcher; Edwin D. W. Moore Standard local control theory, which describes Ca 2+ release during excitation–contraction coupling (ECC), assumes that all ryanodine receptor 2 (RyR2) complexes are equivalent. Findings from our laboratory have called...

...Zheng Liu; Ruiwu Wang; Xixi Tian; Xiaowei Zhong; Jaya Gangopadhyay; Richard Cole; Noriaki Ikemoto; S. R. Wayne Chen; Terence Wagenknecht Naturally occurring mutations in the cardiac ryanodine receptor (RyR2) have been linked to certain types of cardiac arrhythmias and sudden death. Two mutation...

... to secondary lymphoid organs where they initiate primary immune responses . The mechanism and molecules involved in the early steps of Ca 2+ release in dendritic cells have not yet been defined. Here we show that the concomitant activation of ryanodine receptor-induced Ca 2+ release together...

...Spyros Zissimopoulos; Duncan J. West; Alan J. Williams; F. Anthony Lai The ryanodine receptor (RyR) is a widely expressed intracellular calcium (Ca 2+ )-release channel regulating processes such as muscle contraction and neurotransmission. Snapin, a ubiquitously expressed SNARE-associated protein...

... is important for adrenergic-mediated hypertrophy. One function of the mAKAP complex is to facilitate cAMP-dependent protein kinase A-catalyzed phosphorylation of the ryanodine receptor Ca 2+ -release channel. Experiments utilizing inhibition of the ryanodine receptor, RNA interference of mAKAP expression...

...Jonathan-Lee Jones; Deborah F. Reynolds; F. Anthony Lai; Lynda M. Blayney Ryanodine receptor (RyR) Ca 2+ release channels undergo a conformational change between the open and closed states. Its protein modulator, FK506 binding protein 12 (FKBP12), stabilises the channel gating between the four...

...Pieranna Chiarella; Rossella Puglisi; Vincenzo Sorrentino; Carla Boitani; Mario Stefanini Ryanodine receptors (RyRs) are intracellular calcium release channels that are highly expressed in striated muscle and neurons but are also detected in several non-excitable cells. We have studied...

...): all three messengers are equally able to reduce the Ca 2+ concentration inside the NE and this is associated with a transient rise in the nucleoplasmic Ca 2+ concentration. The NE contains ryanodine receptors (RyRs) and Ins(1,4,5) P 3 receptors [Ins(1,4,5) P 3 Rs], and these can be activated...

... in the presence of dihydropyridine Ca 2+ antagonists, which suppress Ca 2+ activity in smooth muscle cells. However, applications of drugs affecting either ryanodine receptors or inositol 1,4,5-trisphosphate receptors terminated [Ca 2+ ] i oscillations at relatively low concentrations. RT-PCR analyses revealed...