...Theresa Fresquez; Beatrice M. Tam; Shannon C. Eshelman; Orson L. Moritz; Michael A. Robichaux; Dusanka Deretic ABSTRACT The Rab11–Rabin8–Rab8 ciliogenesis complex regulates the expansion of cilia-derived light-sensing organelles, the rod outer segments, via post-Golgi rhodopsin transport carriers...

...Abhishek Chadha; Stefanie Volland; Natella V. Baliaouri; Elaine M. Tran; David S. Williams ABSTRACT The photoreceptor outer segment is the most elaborate primary cilium, containing large amounts of rhodopsin (RHO) in disk membranes that grow from a connecting cilium. The movement of RHO along...

...Vasundhara Kandachar; Beatrice M. Tam; Orson L. Moritz; Dusanka Deretic ABSTRACT The Arf4–rhodopsin complex (mediated by the VxPx motif in rhodopsin) initiates expansion of vertebrate rod photoreceptor cilia-derived light-sensing organelles through stepwise assembly of a conserved trafficking...

... receptor, rhodopsin. Rhodopsin and Arf4 bind the regulatory N-terminal dimerization and cyclophillin-binding (DCB)-homology upstream of Sec7 (HUS) domain of GBF1. The complex is sensitive to Golgicide A (GCA), a selective inhibitor of GBF1 that accordingly blocks rhodopsin delivery to the cilia, without...

...Ying Xu; Tao Wang ABSTRACT Endocytosis of G-protein-coupled receptors (GPCRs) and associated channels contributes to desensitization and adaptation of a variety of signaling cascades. In Drosophila melanogaster , the main light-sensing rhodopsin (Rh1; encoded by ninaE ) and the downstream ion...

... cilia are still unknown. Here, we show that the Arf and Rab11 effector FIP3 (also known as RAB11FIP3) promotes the activity of Rab11a and the Arf GTPase-activating protein (GAP) ASAP1 in the Arf4-dependent ciliary transport of the sensory receptor rhodopsin. During its passage out of the photoreceptor...

...) phagocytosis by the RPE provides a unique opportunity to analyse phagosome processing in vivo . In mouse retinae, phagosomes containing stacked rhodopsin-rich discs were identified by immuno-electron microscopy. Early apical phagosomes stained with antibodies against both cytoplasmic and intradiscal domains...

... in the Ca 2+ -dependent visual pigment cycle, which result in accumulation of toxic phosphorylated metarhodopsin–arrestin complexes (M PP –Arr2). Here we show that two interventions, which prevent accumulation of M PP –Arr2, namely rearing under red light or eliminating the C-terminal rhodopsin...

... to combat protein-misfolding disease. References Aguilà., M, Toledo, D., Morillo, M., Dominguez, M., Vaz, B., Alvarez, R., de Lera, A. R. and Garriga, P. ( 2009 ). Structural coupling of 11-cis-7-methyl-retinal and amino acids at the ligand binding pocket of rhodopsin. Photochem. Photobiol. 85...

...Jana Mazelova; Nancy Ransom; Lisa Astuto-Gribble; Michael C. Wilson; Dusanka Deretic The biogenesis of cilia-derived sensory organelles, the photoreceptor rod outer segments (ROS), is mediated by rhodopsin transport carriers (RTCs). The small GTPase Rab8 regulates ciliary targeting of RTCs...

...Nicholas R. Orem; Luxi Xia; Patrick J. Dolph Previously, we have identified a class of retinal degeneration mutants in Drosophila in which the normally transient interaction between arrestin2 (Arr2) and rhodopsin is stabilized and the complexes are rapidly internalized into the cell body...

...Richard S. Saliba; Peter M. G. Munro; Philip J. Luthert; Michael E. Cheetham Mutations in the photopigment rhodopsin are the major cause of autosomal dominant retinitis pigmentosa. The majority of mutations in rhodopsin lead to misfolding of the protein. Through the detailed examination of P23H...

... by heat treatment (37°C) of the fly. Within several hours after the heat induction, the lumens of the rough endoplasmic reticulum (rER) became swollen, and Golgi bodies were disassembled into vesicle clusters. Corresponding to these changes in cell structure, rhodopsin transport was blocked between...

.... In our approach to identify proteins involved in rhodopsin transport and sorting in retinal pho-toreceptors, we have found, using [ 32 P]GTP overlays of 2D gel blots, that six small GTP-binding proteins are tightly bound to the post-Golgi membranes immunoisolated with a mAb to the cytoplasmic domain...

...Dusanka Deretic; David S. Papermaster ABSTRACT The biogenesis of light sensitive membranes in retinal rod photoreceptors involves polarized sorting and tar-geting of newly synthesized rhodopsin to a specialized domain, the rod outer segment (ROS). We have isolated and characterized the population...

... cells suggests that both somatic and ciliary structures are photosensitive. In our search for the photoreceptive molecules, a polyclonal antibody induced in rabbits against frog rhodopsin was found to croos-react with a 63 × 10 3 M τ protein of P. bursaria , by Immunoelectrophoresis. Immunocytochemical...