... form of RhoA ( Fig. S4A,B ). Although the residual GEF activity of mouse ARHGEF10 L548A might be sufficient to rescue Rab8 impairment induced by ARHGEF10 knockdown, our results strongly suggest that ARHGEF10 does not behave as a RhoGEF in the membrane transport pathway. Alternatively, ARHGEF10 might...

... unclear which partners bind simultaneously and may be able to function together. Furthermore, the tight linkage of an ArfGAP and RhoGEF suggests a coordination of GAP and GEF functions. In GIT–PIX-dependent systems, elevated Rac1 and/or Cdc42 activity has been found to be associated with reduced Arf6...

..., triggers radial polarization by inactivating CDC-42 at these sites. The mechanisms responsible for activating CDC-42 at contact-free surfaces are unknown. Here, in an overexpression screen of Rho guanine nucleotide exchange factors (RhoGEFs), which can activate Rho GTPases, we identify CGEF-1 and ECT-2...

... which of them is capable of activating Cdc42 in vivo. By drawing an analogy with other RhoGEFs ( Murayama et al., 2007 ), we speculate that the CB SH3 domain inhibits the DH domain catalytic activity, thereby providing an explanation for why CB2 SH3− can mediate translocation of gephyrin to the plasma...

... Kalirin function throughout development. * Author for correspondence (e-mail: eipper@uchc.edu ) 16 6 2008 © The Company of Biologists Limited 2008 2008 RhoGEF PC12 cells Spectrin-like repeats PP-1 Phosphorylation Roscovitine Calyculin A We thank Martin Schiller...

... exchange factor (RhoGEF). BNIPXL does not recognize the constitutive active G14V and Q63L mutants of RhoA but targets the fast-cycling F30L and the dominant-negative T19N mutants. A second region at the N-terminus of BNIPXL also targets the proline-rich region of Lbc. Whereas overexpression of BNIPXL...