...: Assembly of an irregular contractile actomyosin network that drives secretion from large vesicles in Drosophila is a biphasic process that employs distinct thresholds of activated Rho1 GTPase for actin assembly and myosin II recruitment, and is mediated by RhoGEF2. Drosophila Rho RhoGEF...

... localization on vesicles emerging from the Golgi apparatus. A further proposal suggests that ARHGEF10 function is related to Rab8 localization on these vesicles and cell invasiveness. ARHGEF10 RhoGEF Membrane trafficking RabGTPase Exocytotic vesicles containing newly synthesized secreted...

... signaling molecules, it remains unclear which partners bind simultaneously and may be able to function together. Furthermore, the tight linkage of an ArfGAP and RhoGEF suggests a coordination of GAP and GEF functions. In GIT–PIX-dependent systems, elevated Rac1 and/or Cdc42 activity has been found...

..., triggers radial polarization by inactivating CDC-42 at these sites. The mechanisms responsible for activating CDC-42 at contact-free surfaces are unknown. Here, in an overexpression screen of Rho guanine nucleotide exchange factors (RhoGEFs), which can activate Rho GTPases, we identify CGEF-1 and ECT-2...

... which of them is capable of activating Cdc42 in vivo. By drawing an analogy with other RhoGEFs ( Murayama et al., 2007 ), we speculate that the CB SH3 domain inhibits the DH domain catalytic activity, thereby providing an explanation for why CB2 SH3− can mediate translocation of gephyrin to the plasma...

... Kalirin function throughout development. * Author for correspondence (e-mail: [email protected] ) 16 6 2008 © The Company of Biologists Limited 2008 2008 RhoGEF PC12 cells Spectrin-like repeats PP-1 Phosphorylation Roscovitine Calyculin A We thank Martin Schiller...

... exchange factor (RhoGEF). BNIPXL does not recognize the constitutive active G14V and Q63L mutants of RhoA but targets the fast-cycling F30L and the dominant-negative T19N mutants. A second region at the N-terminus of BNIPXL also targets the proline-rich region of Lbc. Whereas overexpression of BNIPXL...