... a critical role in cell-fate decisions during skeletal development and haematopoiesis in higher vertebrates. Rho GTPases – RhoA, Rac1 and CDC42 – are small G proteins that regulate various key cellular processes, such as actin cytoskeleton formation, the maintenance of cell shape and cell migration. Here, we...

.... Fig. 6. Effects of small Rho GTPases regarding cell spreading on fibronectin- coated or galectin-8-coated substrates. (A–D) Focal adhesions, projected cell area and filopodia in control HeLa-JW cells, and in cells transfected with constitutively active small Rho GTPases, GFP-RhoA-V14, GFP...

... known as MMP14) and Rho GTPases. Moreover, we demonstrate the role of Rab8 in the cell migration process. Indeed, Rab8 is required for EGF-induced cell polarization and chemotaxis, as well as for the directional persistency of intrinsic cell motility. These data reveal that Rab8 drives cell motility...

... Epiboly Cell intercalation Xenopus Lfc Myosin II Rho GTPases Morphogenesis Although neural tube defects are among the most common developmental abnormalities, and more than 200 genes have been implicated in this process in mouse genetic models ( Harris and Juriloff, 2010 ), the molecular...

...Muriel D. David; Dominique Petit; Jacques Bertoglio ABSTRACT Small GTP-binding proteins of the Rho family orchestrate the cytoskeleton remodelling events required for cell division. Guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs) promote cycling of Rho GTPases...

... © 2013. Published by The Company of Biologists Ltd 2013 Rho GTPases Adhesion dynamics Actomyosin Guanine nucleotide exchange factor β1 integrin Asef2 SPATA13 Cell migration is vital for embryonic development and in maintaining homeostasis in the adult ( Vicente-Manzanares...

.../ ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. ERK PI3K Wound healing Rho GTPases Xenopus Wound healing is a complex process in which diverse cell and tissue lineages must work together to restore...

.... , Nonomura   K. , Maekawa   M. , Narumiya   S. ( 2000 ). Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases.   Mol. Pharmacol.   57 , 976 – 983 . Jaffe   A. B. , Hall   A. ( 2005 ). Rho GTPases: biochemistry and biology.   Annu. Rev. Cell Dev. Biol...

...Daniela Alfano; Pia Ragno; M. Patrizia Stoppelli; Anne J. Ridley Urokinase-type plasminogen activator (uPA) and its receptor, uPAR, play important roles in promoting cancer cell adhesion, migration and invasion. Rho GTPases are key coordinators of these processes; the Rho GTPase Rac1 has previously...

... between the actin cytoskeleton and the mitotic spindle during the early stages of mitosis. * Author for correspondence ( cgkoh@ntu.edu.sg ) 8 11 2011 © 2012. 2012 Actin cytoskeleton Astral microtubule Cortical rigidity LIM kinases Spindle orientation Rho GTPases...

... to this work 18 10 2011 © 2012. 2012 Chemotaxis Rho GTPases Immune synapse PDZ Syntenin-1 T cell migration and activation in response to antigen are characterized by morphological changes dictated by the subcellular redistribution of a number of molecules, including chemokine...

... and polarity in Dictyostelium . * Authors for correspondence ( iweber@irb.hr ; faix.jan@mh-hannover.de ) 28 7 2011 © 2012. 2012 Rac1 Cell motility Cell polarization FRET Actin cytoskeleton Rho GTPases Dictyostelium A major role of canonical small GTPases from the Rho...

.... 2C,D ). Because it has been shown that SFKs act downstream of integrins, and that SFKs can regulate activation of Rho GTPases by influencing RhoGDIs ( DerMardirossian et al., 2006 ; Playford and Schaller, 2004 ), we next analyzed the relationship between SFKs and Cdc42 during EC lumen...

...Fanny Momboisse; Etienne Lonchamp; Valerie Calco; Mara Ceridono; Nicolas Vitale; Marie-France Bader; Stéphane Gasman Rho GTPases are crucial regulators of actin cytoskeletal rearrangements and play important roles in many cell functions linked to membrane trafficking processes. In neuroendocrine...

...Rolf-Peter Scholz; Jennifer Regner; Anke Theil; Patrik Erlmann; Gerlinde Holeiter; Ruth Jähne; Simone Schmid; Angelika Hausser; Monilola A. Olayioye Deleted in liver cancer 1 (DLC1) is a Rho-GTPase-activating protein (GAP) that is downregulated in various tumor types. In vitro, DLC1 specifically...

...Stephen D. Smith; Zahara M. Jaffer; Jonathan Chernoff; Anne J. Ridley PAK1 is a member of the p21-activated kinase (PAK) family of serine/threonine kinases that are activated by the Rho GTPases Rac and Cdc42, and are implicated in regulating morphological polarity, cell migration and adhesion. Here...

... in differentiation in siRNA426-expressing keratinocytes plated in the absence of a feeder layer and incubated in high Ca2+ medium 4 days prior to analysis. The percentage of keratinocytes with low forward and side scatter is indicated. The levels of Rho GTPase activity are tightly controlled during...

.... 117 , 3473 -3480. Ridley, A. J. ( 2001 ). Rho GTPases and cell migration. J. Cell. Sci. 114 , 2713 -2722. Riento, K. and Ridley, A. J. ( 2003 ). Rocks: Multifunctional kinases in cell behaviour. Nat. Rev. Mol. Cell Biol. 4 , 446 -456. Sahai, E. and Marshall, C. J. ( 2002...

...Wonshill Koh; Rachel D. Mahan; George E. Davis Rho GTPases regulate a diverse spectrum of cellular functions involved in vascular morphogenesis. Here, we show that Cdc42 and Rac1 play a key role in endothelial cell (EC) lumen and tube formation as well as in EC invasion in three-dimensional (3D...

...Yi Xie; E-Jean Tan; Shimei Wee; Edward Manser; Louis Lim; Cheng-Gee Koh Rho GTPases and their downstream effectors regulate changes in the actin cytoskeleton that underlie cell motility and adhesion. They also participate, with RhoA, in the regulation of gene transcription by activating serum...