...Eike K. Mahlandt; Janine J. G. Arts; Werner J. van der Meer; Franka H. van der Linden; Simon Tol; Jaap D. van Buul; Theodorus W. J. Gadella; Joachim Goedhart ABSTRACT Rho GTPases are regulatory proteins, which orchestrate cell features such as morphology, polarity and movement. Therefore, probing...

... interests. 7 10 2016 1 2 2017 © 2017. Published by The Company of Biologists Ltd 2017 http://www.biologists.com/user-licence-1-1/ Summary: Yeast resists plasma membrane stress by maintaining the cortical localization of Rho GTPase through lipid remodeling mediated by Ypk...

... exocyst complex is implicated in vesicle tethering during exocytosis. Recent works from different fields have revealed its role beyond membrane trafficking. Rab Ral Rho Exocyst Membrane fusion Vesicle tether The exocyst complex comprises eight subunits: Sec3, Sec5, Sec6, Sec8, Sec10...

...Shinichiro Nakahara; Koji Tsutsumi; Takuya Zuinen; Yasutaka Ohta ABSTRACT Rho family small GTPases are essential for the formation of adherens junctions in epithelial cells. Here, we found that FilGAP (also known as ARHGAP24), a Rac-specific Rho GTPase-activating protein, promoted the formation...

... equally to this work. ‡ Author for correspondence ( pts7h@virginia.edu ) Competing interests The authors declare no competing interests. 19 02 2014 27 10 2014 © 2015. Published by The Company of Biologists Ltd 2015 p190RhoGAP Anillin Myosin II Cytokinesis Rho...

... completely. Simultaneous imaging of the vesicle membrane and the actin coat revealed that contraction of the actin coat compresses the vesicle following fusion. This leads to active extrusion of vesicle contents. Initial actin coating of fused vesicles is dependent on activation of Rho and formin-dependent...

...Satoko Sakamoto; Toshimasa Ishizaki; Katsuya Okawa; Sadanori Watanabe; Takatoshi Arakawa; Naoki Watanabe; Shuh Narumiya Regulation of the actin cytoskeleton is crucial for cell morphology and migration. mDia is an actin nucleator that produces unbranched actin filaments downstream of Rho. However...

...Hye-Jin Shin; Seung Bae Rho; Dae Chul Jung; Inn-Oc Han; Eok-Soo Oh; Joo-Young Kim Expression of carbonic anhydrase IX (CA9) was shown to be strongly involved in high incidences of metastasis and poor prognosis in various human tumors. In this study, we investigated the possible role for CA9...

... , 5352 - 5364 . Hildebrand J. D. , Taylor J. M. , Parsons J. T. ( 1996 ). An SH3 domain-containing GTPase-activating protein for Rho and Cdc42 associates with focal adhesion kinase . Mol. Cell Biol. 16 , 3169 - 3178 . Iwanicki M. P. , Vomastek T. , Tilghman R...

... to cadherins and Rho-family GTPases. Fig. 7. δ-catenin depletion leads to reduced cadherin functions. (A) Immunoblotting shows that δ-catenin depletion reproducibly leads to reduced C-, E- and N-cadherin levels, whereas p120-catenin and β-catenin levels are not significantly altered.(B) Calcium...

... E-cadherin Rho α3β1 integrin A431 cells were cultured in standard medium (DME with 10% FBS, 2 mM L-glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin). Gaps were inscribed in freshly confluent monolayers using a yellow micropipet tip, and the cultures were refed with serum-free...

..., such as the adaptor TNF-receptor-associated factor 4 and the ectodysplasin A receptor (and its ligand and adaptors), have dedicated morphogenetic roles. The mechanism by which TNF signalling affects cell shape is not yet fully understood, but Rho-family GTPases have a central role. The fact that the components...

... to a several fold increase in free G-actin along with an increase in total G-actin. These results indicate that in cells, the actin nucleation frequency of mDia1 is enhanced not only by Rho, but also strongly through increased catalytic efficiency of the FH2 domain. Consistently, frequent actin nucleation...

... stress fibers, causing a typical elongated, contractile morphology. Extensive remodeling of adhesions in fibroblasts also takes part in fibronectin fibrillogenesis, a process that depends on Rho-mediated contractility and results in the formation of a fibronectin matrix. Our current study shows that all...

... by FRAP. Complete microtubule disassembly or selective loss of astral microtubules resulted in wave-like contractile behavior of actin in the non-equatorial cortex, and mislocalization of myosin II and Rho. FRAP experiments showed that both contractility and actin polymerization contributed to the wave...

... suppress cellular transformation by preventing sustained Rho activation in concert with restricting RhoA and Lbc binding via its BCH domain. This could provide a general mechanism for regulating RhoGEFs and their target GTPases. * Author for correspondence (e-mail: dbslowbc@nus.edu.sg ) 13 2...

..., and a crucial role for Rac in the stabilization of protrusions required for cell motility. ‡ Author for correspondence (e-mail: backer@aecom.yu.edu ) * These authors contributed equally to this work 18 6 2007 © The Company of Biologists Limited 2007 2007 Ras Rac Cdc42 Rho...

.... Indeed, TTC3 overexpression leads to strong inhibition of neurite extension, which can be reverted by CIT-K RNAi. Conversely, TTC3 knockdown stimulates neurite extension in the same cells. Finally, we find that Rho, but not Rho kinase, is required for TTC3 differentiation-inhibiting activity. Our results...

...Ya-yu Chuang; Aline Valster; Salvatore J. Coniglio; Jonathan M. Backer; Marc Symons Wrch-1 (Wnt-regulated Cdc42 homolog) is a new member of the Rho family that was identified as a gene transcriptionally upregulated by Wnt-1. Wrch-1 has no detectable GTPase activity and displays very high intrinsic...

...Akiko Mammoto; Sui Huang; Donald E. Ingber Cytoskeleton-dependent changes in the activity of the small GTPase Rho mediate the effects of cell shape on cell function; however, little is known about how cell spreading and related distortion of the cytoskeleton regulate Rho activity. Here we show...