...Abhishek Prakash Shinde; Jitka Kučerová; Joel Bryan Dacks; Jan Tachezy ABSTRACT Early endosomes sort transmembrane cargo either for lysosomal degradation or retrieval to the plasma membrane or the Golgi complex. Endosomal retrieval in eukaryotes is governed by the anciently homologous retromer...

...Shuwei Xie; Carter Dierlam; Ellie Smith; Ramon Duran; Allana Williams; Angelina Davis; Danita Mathew; Naava Naslavsky; Jyoti Iyer; Steve Caplan ABSTRACT The mammalian retromer consists of subunits VPS26 (either VPS26A or VPS26B), VPS29 and VPS35, and a loosely associated sorting nexin (SNX...

... to the trans-Golgi network (TGN). At high copper, ATP7B colocalizes with endolysosomal markers and with a core member of retromer complex, VPS35. Knocking down VPS35 did not abrogate the copper export function of ATP7B or its copper-responsive anterograde trafficking to vesicles; rather upon subsequent copper...

...Rachel Curnock; Peter J. Cullen ABSTRACT The concentration of essential micronutrients, such as copper (used here to describe both Cu + and Cu 2+ ), within the cell is tightly regulated to avoid their adverse deficiency and toxicity effects. Retromer-mediated sorting and recycling of nutrient...

...Ashley J. Evans; James L. Daly; Anis N. K. Anuar; Boris Simonetti; Peter J. Cullen ABSTRACT Human retromer, a heterotrimer of VPS26 (VPS26A or VPS26B), VPS35 and VPS29, orchestrates the endosomal retrieval of internalised cargo and promotes their cell surface recycling, a prototypical cargo being...

... CLN3 and Rab7A, a small GTPase that regulates several functions at late endosomes. We confirmed this interaction in live cells and found that CLN3 is required for the efficient endosome-to-TGN trafficking of the lysosomal sorting receptors because it regulates the Rab7A interaction with retromer...

... . The mechanism of action in the skin is independent from that previously described for this protein in retrograde trafficking. RAB-6.2 Epidermis Retromer RAB6 Exocyst Intracellular trafficking is crucial for development, cellular function and homeostasis, and is regulated by many families...

... attributed. Summary: A potential scaffolding function for SNX21 paves the way for a more-detailed mechanistic analysis of the role(s) of this protein in endosomal biology. Huntingtin Retromer Septin Sorting nexin Wellcome Trust 10.13039/100010269 104568/Z/14/Z Medical...

... that the effect of this phosphorylation of SNX27 phenocopies the effects seen upon loss of SNX27 function, affecting PM trafficking of cargo proteins that bind SNX27–retromer. Additionally, CT destabilizes retromer function by decreasing the amount of core retromer proteins. These effects of CT can be partially...

...Matthew N. J. Seaman; Aamir S. Mukadam; Sophia Y. Breusegem ABSTRACT The retromer complex is a vital component of the endosomal protein sorting machinery necessary for sorting into both the endosome-to-Golgi retrieval pathway and also the endosome-to-cell-surface recycling pathway. Retromer...

... of the polycystins through the biosynthetic and endosomal membrane networks remain to be fully elucidated. Here, we describe an interaction between PC2 and retromer, a master controller for the sorting of integral membrane proteins through the endo-lysosomal network. We show that association of PC2 with retromer...

... of cargo proteins, mediated by the retromer complex. Recent work has led to significant progress in understanding the role of Arabidopsis retromer subunits in recycling vacuolar sorting receptors and plasma membrane proteins. As a consequence, members of the sorting nexin (SNX) protein family...

... in evolution, such as retromer, which is involved in the recycling of a diverse set of cargoes via the retrograde trafficking route. Here, in an RNAi-based loss-of-function study, we identified that suppression of SNX12 leads to a severe blockage in CIM6PR (also known as IGF2R) transport and alters...

...Graziana Modica; Olga Skorobogata; Etienne Sauvageau; Adriano Vissa; Christopher M. Yip; Peter K. Kim; Hugo Wurtele; Stephane Lefrancois ABSTRACT Retromer is a multimeric protein complex that mediates endosome-to-trans-Golgi network (TGN) and endosome-to-plasma membrane trafficking of integral...

... mechanism for EHD1 function. Recent studies have demonstrated that VPS35 and the retromer complex influence mitochondrial homeostasis either by Mul1-mediated ubiquitylation and degradation of the fusion protein Mfn2, or by removal of inactive Drp1 from the mitochondrial membrane. We demonstrate that EHD1...

...Arunas Kvainickas; Ana Jimenez Orgaz; Heike Nägele; Britta Diedrich; Kate J. Heesom; Jörn Dengjel; Peter J. Cullen; Florian Steinberg ABSTRACT Retromer and the associated actin-polymerizing WASH complex are essential for the endocytic recycling of a wide range of integral membrane proteins...

...Henning Arlt; Fulvio Reggiori; Christian Ungermann ABSTRACT Endosomes are dynamic organelles that need to combine the ability to successfully deliver proteins and lipids to the lysosome-like vacuole, and recycle others to the Golgi or the plasma membrane. We now show that retromer, which...

...Zhi-Hui Deng; Timothy S. Gomez; Douglas G. Osborne; Christine A. Phillips-Krawczak; Jin-San Zhang; Daniel D. Billadeau ABSTRACT The pentameric WASH complex is best known for its role in regulating receptor trafficking from retromer-rich endosomal subdomains. FAM21 functions to stabilize the WASH...

...Ian J. McGough; Florian Steinberg; Matthew Gallon; Ayaka Yatsu; Norihiko Ohbayashi; Kate J. Heesom; Mitsunori Fukuda; Peter J. Cullen ABSTRACT Retromer is a protein assembly that orchestrates the sorting of transmembrane cargo proteins into endosome-to-Golgi and endosome-to-plasma-membrane...

...Caroline L. Freeman; Geoffrey Hesketh; Matthew N. J. Seaman ABSTRACT Retromer is a vital element of the endosomal protein sorting machinery and comprises two subcomplexes that operate together to sort membrane proteins (cargo) and tubulate membranes. Tubules are formed by a dimer of sorting nexins...