... affect their trafficking between the ER and the Golgi in the forward and backward directions. Anterograde transport Retrograde transport Cargo capture Membrane proteins Partitioning Sorting signals Twenty to thirty years ago, a handful of seminal studies ( Wieland et al., 1987...

... are important sorting stations for some retrograde cargo. SMAP2, a GTPase-activating protein (GAP) for Arf1 with a putative clathrin-binding domain, has previously been shown to participate in the retrograde transport of the cholera toxin B-subunit (CTxB) from recycling endosomes. Here, we found that clathrin...

... delays Brefeldin-A-induced Golgi-to-ER retrograde transport, phenocopying cells with mutations or silencing of the conserved oligomeric Golgi (COG) complex. Co-immunoprecipitation and in vitro binding experiments reveals that TMEM115 interacts with the COG complex, and might self-interact to form dimers...

...: some is recycled to the plasma membrane via Rab11-labelled recycling endosomes; the remaining PrP Sc is subject to retromer-mediated retrograde transport to the Golgi. This pathway leads to lysosomal degradation, and we show that this is the dominant PrP Sc degradative mechanism in the early stages...

.... Overall, these data show that furin uses the Rab9-dependent pathway from late endosomes and that retrograde transport directly from early endosomes is dependent on both the transmembrane domain and the cytoplasmic tail. References Amessou M. , Fradagrada A. , Falguieres T. , Lord...

...Nicolas Carvou; Roman Holic; Michelle Li; Clare Futter; Alison Skippen; Shamshad Cockcroft Vesicles formed by the COPI complex function in retrograde transport from the Golgi to the endoplasmic reticulum (ER). Phosphatidylinositol transfer protein β (PITPβ), an essential protein that possesses...

... of the endosomal β-cleavage pathway reduces translocation of AICD to these nuclear AFT complexes. AICD signaling further depends on active transport along microtubules and can be modulated by interference with both anterograde and retrograde transport systems. Nuclear signaling by endogenous AICD in primary...

... retrograde transport of GFP-tagged galactosyltransferase, GT-GFP, but not the COPI-dependent retrograde transport of p58/ERGIC53. Furthermore, we found that Yip1A knockdown resulted in the dissociation of Rab6 from the membranes. These results suggested that Yip1A has a role in COPI-independent retrograde...

... for efficient retrograde transport of STxB from endosomes to the Golgi ( Bujny et al., 2007 ), it is of particular interest to note that a recent study has demonstrated that EHD1 can interact with the retromer subunit VPS35, and that overexpression of EHD1 mutants that are incapable of proper ATP hydrolysis...

... vesicles with the ER in retrograde transport remains elusive. Here, we show that in yeast Ypt1p is essential for retrograde transport from the Golgi to the ER. Retrieval of gpαF-HDEL (glycolylated pro-α-factor with an HDEL tag at the C-terminus) was blocked in Δ ypt1/SLY1-20 membranes at the restrictive...

...Miriam V. Bujny; Vincent Popoff; Ludger Johannes; Peter J. Cullen The mammalian retromer complex is a multi-protein complex that regulates retrograde transport of the cation-independent mannose 6-phosphate receptor (CI-MPR) from early endosomes to the trans Golgi network (TGN). It consists of two...

...-subunit, a well-studied retrograde transport cargo. As previously described for clathrin, we find that interfering with Vps26 expression inhibits retrograde transport of the Shiga toxin B-subunit to the TGN. Under these conditions, endosomal tubules that take the Shiga toxin B-subunit out of transferrin...

...Mohamed Amessou; Alexandre Fradagrada; Thomas Falguières; J. Michael Lord; Daniel C. Smith; Lynne M. Roberts; Christophe Lamaze; Ludger Johannes Retrograde transport allows proteins and lipids to leave the endocytic pathway to reach other intracellular compartments, such as trans-Golgi network...

... formed Golgi elements. * Author for correspondence (e-mail: [email protected] ) 2 3 2005 © The Company of Biologists Limited 2005 2005 Dynein Dynactin Centrosome Retrograde transport Endosome The Golgi complex plays a central role in glycoprotein processing...

...Alice Chen; Ramzey J. AbuJarour; Rockford K. Draper Cholera toxin, Shiga toxin and ricin are examples of protein toxins that require retrograde transport from the Golgi complex into the endoplasmic reticulum (ER) to express their cytotoxic activities and different toxins appear to use different...

.... * Author for correspondence 03 11 1999 01 09 1999 © 1999 by Company of Biologists 1999 Bet1p Bos1p ER-Golgi v -SNARE Retrograde transport COPI The machineries that mediate the bidirectional transport between the endoplasmic reticulum (ER) and Golgi are conserved...

... cells antibodies raised against the cytoplasmically exposed tail of the KDEL receptor. Immunofluorescence confirmed that these antibodies prevented the retrograde transport of the KDEL receptor from the Golgi complex to the ER, and this in turn reduced the cytotoxicity of PE, but not that of SLT-1...

..., very little of this protein is cleaved by Kex2p. Efficient cleavage, however, occurs in mutants defective in the retrograde transport of different ER-resident proteins indicating that Sec22-α rapidly reaches the late Golgi of these cells. These mutants ( sec20-1, sec21-1, sec27-1 and ufe1-1 ) reveal...

... in untreated cells. These data suggest a regulatory role for both αs and αi trimeric G proteins in the normal Golgi-ER retrograde transport taking place in intact cells. * Author for correspondence 09 09 1994 09 12 1994 © 1995 by Company of Biologists 1995 Golgi complex...

... whether MG160 acquires sialic acid residues in the trans cisternae or trans -Golgi network (TGN) prior to its retrograde transport, we have examined the effects of brefeldin A (BFA) on the post-translational processing of MG160, and the distribution of internalized wheat germ agglutinin covalently linked...