... is required for zebrafish retinal development, including Müller glia development and protection from cell death. Semaphorin Eye development Retina Reverse signaling Zebrafish National Science Foundation http://dx.doi.org/10.13039/100000001 1021795 126786 1625154 Fig. 5...

... result in diabetic embryopathy. While many studies have examined the teratogenic effects of maternal diabetes on fetal heart development, little is known about the consequences of maternal hyperglycemia on the development of the embryonic retina. To address this question, we investigated retinal...

... imaging with quantitative electron-density contrast. Here, we imaged, at cryogenic temperature and without sectioning, cellular and subcellular structures of a chemically fixed and stained wild-type mouse retina, including axons and synapses, with complete isotropic 3D information over tens of microns...

...Silvia Dragoni; Bruna Caridi; Eleni Karatsai; Thomas Burgoyne; Mosharraf H. Sarker; Patric Turowski ABSTRACT Many neuronal and retinal disorders are associated with pathological hyperpermeability of the microvasculature. We have used explants of rodent retinae to study acute neurovascular...

... and myosin-7a, thus supporting a capture mechanism that involves both microtubules and actin motors. Melanosome Retina Dynein Kinesin-1 Myosin-7a National Institutes of Health 10.13039/100000002 R01EY027442 Melanosomes are organelles that originate from endosomes and contain...

... that follows photoreceptor shedding in wild-type retina. Increasing ANXA5 in cells lacking αvβ5 or increasing αvβ5 in cells lacking ANXA5 does not affect particle binding. Association of cytosolic ANXA5 and αvβ5 integrin in RPE in culture and in vivo further supports their functional interdependence. Silencing...

... protein and mutations in the human PCNT gene are associated with several diseases, including ciliopathies. Pcnt plays a crucial role in ciliary development in olfactory receptor neurons, but its function in the photoreceptor-connecting cilium is unknown. We downregulated Pcnt in the retina ex vivo...

... tolerance to abiotic stresses, inhibits neovascularization by suppressing the angiogenic phenotypes of endothelial cells and macrophages, suggesting its potential usefulness in anti-angiogenic therapy. Abscisic acid Angiogenesis Retina Oxygen-induced retinopathy Hyaloid vessel Macrophage...

..., is also dependent on glycylation. In contrast to many other tissues, only one glycylase, TTLL3, is expressed in retina. Ttll3 −/− mice lack glycylation in photoreceptors, which results in shortening of connecting cilia and slow retinal degeneration. Moreover, absence of glycylation results in increased...

...David Jimeno; Carmela Gómez; Nuria Calzada; Pedro de la Villa; Concepción Lillo; Eugenio Santos ABSTRACT Detailed immunocytochemical analyses comparing wild-type (WT), GRF1-knockout (KO), GRF2-KO and GRF1/2 double-knockout (DKO) mouse retinas uncovered the specific accumulation of misplaced...

...; however, the mechanisms underlying formation of Cx36-containing electrical synapses are unknown. We focus on homocellular and heterocellular gap junctions formed by an AII amacrine cell, a key interneuron found in all mammalian retinas. In mice lacking native Cx36 but expressing a variant tagged...

... complexes. Multiple splice variants of 4.1G were cloned from bovine retina. A smaller splice variant of 4.1G selectively interacted with CNG channels not associated with peripherin-2–CNG channel complex. A combination of truncation studies and domain-binding assays demonstrated that CNG channels selectively...

... of the heart and in certain neurons of the retina and brain. General and cardiomyocyte-directed deficiencies of Cx45 in mice lead to lethality on embryonic day 10.5 as a result of cardiovascular defects. Neuron-directed deletion of Cx45 leads to defects in transmission of visual signals. Connexin-36 ( Cx36...

... findings to the retina, and show that meteorin increases GFAP expression in Müller glia. Together, our results suggest that meteorin promotes GFAP-positive glia formation by mediating the Jak-STAT3 signaling pathway during both cortical stem cell differentiation and retinal glia development. * Author...

.... ( 2005 ). Cellular remodeling in mammalian retina: results from studies of experimental retinal detachment. Prog. Retin. Eye Res. 24 , 395 -431. Fukuda, H., Takahashi, J., Watanabe, K., Hayashi, H., Morizane, A., Koyanagi, M., Sasai, Y. and Hashimoto, N. ( 2006 ). Fluorescence-activated cell...

.... The developing retina contains committed precursors characterized by their ability to divide and expression of proneural genes, such as Mash1 ( Ahmad et al., 1998 ). These precursors are malleable and when exposed to conditions that promote the maintenance of retinal stem cells rather than...

...Yasuo Ouchi; Yoko Tabata; Ken-ichi Arai; Sumiko Watanabe Although there have been many studies on the regulation of neurite extension in mouse brain, such a mechanism in neural retina has remained to be clarified. To delineate the role of Wnt signaling in retinal development, we used a retrovirus...

... synergistically to promote a continuous network of tight junctions with higher electrical resistance. Independently, pituitary extract or the secretory products of either embryonic fibroblasts or the retina promoted the formation of tight junctions. In combination, three effects were identified. With basally...

... and in the olfactory epithelium. Antibodies to dynein 2 HC, LIC3 and a component of IFT particles strongly stained the ependymal layer lining the lateral ventricles. Both dynein 2 HC and LIC3 staining was also observed associated with connecting cilia in the retina and within primary cilia of non-neuronal cultured...

... of pigmented quail cells, E8 dissected from the pigmented layer of the retina (cells from RPE), the epitope-tagged QNR-71 was localized in dotted peripheral structures ( Fig. 2a ) that, unexpectedly, did not overlap with pigmented melanosomes, also referred as stage III and IV melanosomes (Raposo et al, 2001...