..., accumulate in nascent T. cruzi vacuoles and at sites of mechanical wounding. Exo70 or Sec8 depletion inhibits T. cruzi invasion and Ca 2+ -dependent resealing of mechanical wounds, but does not affect the repair of smaller lesions caused by pore-forming toxins. Thus, T. cruzi invasion and mechanical lesion...

... role for tethering: it can promote the formation of large vesicular fusion products, such as those required for successful resealing. We have, moreover, identified several proteins that may be critical to this tethering mechanism. * Author for correspondence (email: [email protected]...

...Katsuya Miyake; Paul L. McNeil; Kazunori Suzuki; Rikiya Tsunoda; Naonori Sugai Plasma membrane disruption is a common form of cell injury in many normal biological environments, including many mammalian tissues. Survival depends on the initiation of a rapid resealing response that is mounted only...

...Paul L. McNeil; Steven S. Vogel; Katsuya Miyake; Mark Terasaki ABSTRACT Vesicle-vesicle fusion initiated in cell cytoplasm by high Ca 2+ can rapidly erect large membrane boundaries. These might be used as a ‘patch’ for resealing plasma membrane disruptions. Three central predictions of this ‘patch...