...., 2016 ; Del Rosario et al., 2019 ). Similarly, the apicomplexan nucleus shows impressive deformations during replication so that it is distributed equally to the forming daughter cells, suggesting the integration of cytoskeletal and nuclear functions ( Suvorova et al., 2015 ). We identified...

... regarding replication defects, as determined by measuring dUTP incorporation in the germline. Interestingly, this specific thp-1 mutant phenotype can be partially rescued by overexpression of RNase H. Furthermore, both mutants show a mild increase in phosphorylation of histone H3 at Ser10 (H3S10P), a mark...

... and structural inheritance over successive generations. This was correlated with a major invasion deficiency at early stages of CEN2 knockdown, and replication defects at later stages. These results indicate that CEN2 is incorporated into multiple cytoskeletal structures to serve distinct functions...

... the spatiotemporal organisation of rDNA replication in Arabidopsis thaliana . To determine the subnuclear distribution of rDNA and the progression of its replication during the S phase, we apply 5-ethynyl-2′-deoxyuridine (EdU) labelling, fluorescence-activated cell sorting, fluorescence in situ hybridization...

... the nucleus to the cytoplasm. More interestingly, activated SIRT1 subsequently binds with the EV71 3D pol protein (a viral RNA-dependent RNA polymerase, RdRp) to repress the acetylation and RdRp activity of 3D pol , resulting in the attenuation of viral genome replication. Moreover, SIRT1 interacts...

... replication and DNA damage repair are unclear, particularly without induced replicative stress. We have used a cellular model of conditionally inactive polycomb E3 ligases (RING1A and RING1B), which monoubiquitylate lysine 119 of histone H2A (H2AK119Ub), to examine DNA replication in unperturbed cells. We...

...MaryClare F. Rollins; Dana M. van der Heide; Carla M. Weisend; Jean A. Kundert; Kristin M. Comstock; Elena S. Suvorova; Mario R. Capecchi; Gary F. Merrill; Edward E. Schmidt Cells require ribonucleotide reductase (RNR) activity for DNA replication. In bacteria, electrons can flow from NADPH to RNR...

... stiffness plays key roles in adhesion and cytoskeletal organization, an important open question concerns the influence of substrate elasticity on replication and transcription. To answer this major question, polyelectrolyte multilayer films were used as substrate models with apparent elastic moduli ranging...

... is not required for the completion of mitosis, but is an important regulator of G1 phase and is required for efficient DNA replication in human and mouse somatic cells. * Author for correspondence ( [email protected] ) 10 9 2009 © The Company of Biologists Limited 2009 2009 FZR1...

...Fayza Daboussi; Sylvain Courbet; Simone Benhamou; Patricia Kannouche; Malgorzata Z. Zdzienicka; Michelle Debatisse; Bernard S. Lopez Faithful genome transmission requires a network of pathways coordinating DNA replication to DNA repair and recombination. Here, we used molecular combing to measure...

... by the mitochondrial genome (mtDNA). MtDNA replication is regulated by the nuclear-encoded mitochondrial transcription factor A (TFAM) and the mitochondrial-specific DNA polymerase gamma, which consists of a catalytic (POLG) and an accessory (POLG2) subunit. Differentiation of pluripotent embryonic stem cells (ESCs...

... determined that early after infection, UBF associates with the replication of viral DNA, preferentially associating with the ends of the linear viral genome, and that addition of anti-UBF serum to in vitro replication assays markedly reduced viral DNA replication. Regions of UBF important...

... correlated with aberrant localisation of initiation of DNA replication within the nucleus and an increased fraction of cells in mitosis. Chromosomal fragmentation and other aberrations, including shorter, thicker chromatids, end-to-end fusion, and loss of a chromatid, suggest that reduced B-Myb activity...

...Maria A. Marchetti; Martin Weinberger; Yota Murakami; William C. Burhans; Joel A. Huberman Previous studies have indicated that replication stress can trigger apoptosis-like cell death, accompanied (where tested) by production of reactive oxygen species (ROS), in mammalian cells and budding yeast...

...Noriaki Shimizu; Kenta Shingaki The chromosomal G/R bands are alternating domains differing in their nucleotide sequence biases. The bands are also related to the time of replication: pulse-labeling during S phase makes the replication sites as visible as replication bands that are close to the G/R...

...Marion Ritzi; Kristina Tillack; Jeannine Gerhardt; Elisabeth Ott; Sibille Humme; Elisabeth Kremmer; Wolfgang Hammerschmidt; Aloys Schepers The sequential binding of the origin recognition complex (ORC), Cdc6p and the minichromosome maintenance proteins (MCM2-7) mediates replication competence...

... may represent abnormal DNA replication that is a specific feature of the baf loss-of-function phenotype. If this is the case it would suggest that BAF has a role in events leading to or including S phase, and combined with the fact that others have demonstrated that BAF is localized in both...

... of a role for chaperones in DNA replication in higher eukaryotes. In embryos prior to cellularisation where the protein was most abundant, Dpit47 protein was visible throughout the embryo in the nucleus and cytoplasm (data not shown). After cellularisation, however, the protein was seen predominantly...

...Jan Mal ínský; Karel Koberna; David Stan ěk; Martin Ma šata; Ivan Votruba; Ivan Ra ška ABSTRACT Earlier studies have established that the average speed of a replication fork is two to three times slower in early S-phase than in late S-phase and that the intracellular 2′-deoxyribonucleoside 5...

... the progression through an individual assembly reaction to its active participation in a marking system. This marking system could be exploited at the crossroads of DNA replication and repair to monitor genome integrity and to define particular epigenetic states. * Author for correspondence (e...