...Kseniya Ustinova; Felix Ruhnow; Maria Gili; Thomas Surrey ABSTRACT Mitotic spindle assembly during cell division is a highly regulated process. Ran-GTP produced around chromosomes controls the activity of a multitude of spindle assembly factors by releasing them from inhibitory interaction...

... size regulation. Although nuclear import rates generally positively correlate with nuclear size, NTF2 levels negatively affect nuclear size, despite the role of NTF2 (also known as NUTF2) in nuclear recycling of the import factor Ran. We show that binding of Ran to NTF2 is required for NTF2 to inhibit...

... trafficking into the specialized flagellar/ciliary compartments, although the regulatory mechanisms controlling Ran activity at the flagellum remain unclear. The unicellular parasite Trypanosoma brucei contains a single flagellum necessary for cell movement, division and morphogenesis. Correct flagellum...

... spindle assembly, their biochemical function is not yet certain. RanBP2 may enhance the stimulation of GTP hydrolysis on Ran by RanGAP1, reduce local RanGTP concentrations, and thereby promote the release of active SAFs from Crm1. Alternatively, RanBP2–RanGAP1 may act as an effector of RanGTP...

...Dmitry Yudin; Mike Fainzilber The GTPase Ran is best known for its crucial roles in the regulation of nucleocytoplasmic transport in interphase cells and in the organization of the spindle apparatus during mitosis. A flurry of recent reports has now implicated Ran in diverse cytoplasmic events...

..., the regulation of apoptotic microtubule assembly is not understood. Here, we demonstrate that microtubule assembly depends upon the release of nuclear RanGTP into the apoptotic cytoplasm because this process is blocked in apoptotic cells overexpressing dominant-negative GDP-locked Ran (T24N). Actin–myosin-II...

...Petr Kalab; Rebecca Heald The GTPase Ran has a key role in nuclear import and export, mitotic spindle assembly and nuclear envelope formation. The cycling of Ran between its GTP- and GDP-bound forms is catalyzed by the chromatin-bound guanine nucleotide exchange factor RCC1 and the cytoplasmic Ran...

...Fiona E. Hood; Paul R. Clarke RCC1 is the guanine nucleotide exchange factor for Ran GTPase. Generation of Ran-GTP by RCC1 on chromatin provides a spatial signal that directs nucleocytoplasmic transport, mitotic spindle assembly and nuclear envelope formation. We show that RCC1 is expressed...

... in animal cells; the work has revealed that many kinetochore components are highly dynamic and that some cycle between kinetochores and spindle poles along microtubules. Further studies of the kinetochore-microtubule interface are illuminating: (1) the role of the Ndc80 complex and components of the Ran...

... destabilizing agent. Ned1 protein, which was phosphorylated in a growth-related manner, interacted in a yeast two-hybrid system with Dis3 as well as with Pim1/RCC1 (nucleotide exchange factor for Ran). Ned1 also interacted with an essential nucleoporin, a probable homologue of mammalian Nup98/96. The ned1 gene...

...-expressing cleavage embryos; (2) used it in the nuclear import assay system with digitonin-permeabilized HeLa cells; and (3) used it in solutions to study Ketel-Ran interactions. 13 1 2002 © The Company of Biologists Limited 2002 2002 Importin-β Nuclear envelope formation Ran...

...Anna Matynia; Sandra S. Salus; Shelley Sazer The Ran GTPase is an essential protein that has multiple functions in eukaryotic cells. Fission yeast cells in which Ran is misregulated arrest after mitosis with condensed, unreplicated chromosomes and abnormal nuclear envelopes. The fission yeast sns...

...Francisco J. Nicolás; William J. Moore; Chuanmao Zhang; Paul R. Clarke Ran is a multifunctional small GTPase of the Ras superfamily that plays roles in nucleocytoplasmic transport, mitotic spindle assembly and nuclear envelope formation. By screening a Xenopus oocyte cDNA library for Ran-GTP...

...Chuanmao Zhang; Mike Hughes; Paul R. Clarke ABSTRACT Ran is an abundant GTPase of the Ras superfamily that is highly conserved in eukaryotes. In interphase cells, Ran is mainly nuclear and thought to be predominantly GTP-bound, but it is also present in the cytoplasm, probably GDP-bound...

...Mike Hughes; Chuanmao Zhang; Johanna M. Avis; Christopher J. Hutchison; Paul R. Clarke ABSTRACT The Ran GTPase plays a critical role in nucleocytoplasmic transport and has been implicated in the maintenance of nuclear structure and cell cycle control. Here, we have investigated its role in nuclear...

...Francisco J. Nicolás; Chuanmao Zhang; Mike Hughes; Martin W. Goldberg; Sandra J. Watton; Paul R. Clarke Ran is a nuclear GTPase implicated in nucleocytoplasmic transport, the maintenance of nuclear structure, mRNA processing, and cell cycle regulation. By two-hybrid interaction in yeast, we have...

...Alessandra Battistoni; Giulia Guarguaglini; Francesca Degrassi; Carmine Pittoggi; Antonella Palena; Gigliola Di Matteo; Claudio Pisano; Enrico Cundari; Patrizia Lavia ABSTRACT RanBPI is a molecular partner of the Ran GTPase, which is implicated in the control of several processes, including DNA...

...Nobutaka Nakashima; Naoyuki Hayashi; Eishi Noguchi; Takeharu Nishimoto ABSTRACT In order to identify a protein interacting with RCC1, a guanine nucleotide-exchange factor for the nuclear GTPase Ran, we isolated a series of cold-sensitive suppressors of mtr1-2 , a temperature-sensitive mutant...

...Achim Dickmanns; F. Ralf Bischoff; C. Marshallsay; R. Lührmann; Herwig Ponstingl; Ellen Fanning ABSTRACT The nuclear protein regulator of chromosome condensation 1 (RCC1) stimulates guanine nucleotide exchange on a protein, Ran, that is required for nuclear protein import. In the present report, we...