...Karolina Sapoń; Rafał Mańka; Teresa Janas; Tadeusz Janas ABSTRACT The formation of membrane vesicles is a common feature in all eukaryotes. Lipid rafts are the best-studied example of membrane domains for both eukaryotes and prokaryotes, and their existence also is suggested in Archaea membranes...

... concentrations, translocation of cytoplasmic phospholipase A 2 (cPLA 2 , also known as PLA2G4A) to lipid rafts, and activation of cPLA 2 . The formation of Aβ-PrP C complexes is controlled by the cholesterol ester cycle. In this study, Aβ activated cholesterol ester hydrolases, which released cholesterol from...

...Ji Hyun Kim; Ashutosh Singh; Maurizio Del Poeta; Deborah A. Brown; Erwin London ABSTRACT Ordered lipid domains (rafts) in plasma membranes have been hypothesized to participate in endocytosis based on inhibition of endocytosis by removal or sequestration of cholesterol. To more carefully...

...Romain Galmes; Jean-Louis Delaunay; Michèle Maurice; Tounsia Aït-Slimane Summary Targeting of glycosyl-phosphatidylinositol (GPI)-anchored proteins (GPI-APs) in polarized epithelial cells depends on their association with detergent-resistant membrane microdomains called rafts. In MDCK cells, GPI...

...) in the plasma membrane. Biochemical data suggest that ACs reside in membrane rafts and could compartmentalize intermediary scaffolding proteins and associated regulatory elements. However, little is known about the organization or regulation of the dynamic behaviour of ACs in a cellular context. The present...

[email protected] ; [email protected] ) 18 9 2008 © The Company of Biologists Limited 2008 2008 DRMs GPI-anchored proteins Oligomerisation Rafts Sorting Glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) are directly targeted to the apical domain of the plasma membrane...

...Carolin Neumann-Giesen; Inga Fernow; Monia Amaddii; Ritva Tikkanen Cholesterol and sphingolipid-rich membrane microdomains or rafts have been shown to be involved in signaling through many growth factor receptors but the molecular details of these processes are not well understood. The reggie...

...Tatiana Rogasevskaia; Jens R. Coorssen Membrane microdomains or `rafts' are suggested to act as regulators of the exocytotic process and also appear to be the sites of Ca 2+ -triggered membrane fusion. Microdomains are postulated to maintain the localization of `efficiency' factors, including Ca 2...

..., which have been used as a model system for studies of sorting mechanisms. The clustering of lipid rafts through the oligomerization of raft components could be utilized for segregating apical from basolateral cargo and for the generation of intracellular transport carriers. Besides functioning...

... dynamics. Here, we have studied the relationships between tetraspanins, cytoskeleton and raft microdomains, and their relevance in T-cell signaling. Localization studies and density-gradient flotation experiments indicate that part of tetraspanins localizes in raft microdomains linked to the actin...

... of tissues expressing caveolin, SR-BI is localized in caveolae. We show using detergent-free sucrose gradients that SR-BI is found in membrane rafts devoid of caveolin-1 in the human hepatoma HepG2 cell. Perturbation of the structure of HepG2 cell membrane rafts with cholesterol oxidase or sphingomyelinase...

.... ( 1989 ). All ras proteins are polyisoprenylated but only some are palmitoylated. Cell 57 , 1167 -1177. Janes, P. W., Ley, S. C., Magee, A. I. and Kabouridis, P. S. ( 2000 ). The role of lipid rafts in T cell antigen receptor (TCR)signalling. Semin. Immunol. 12 , 23 -34. Kenworthy, A. K...

... that the EGFR is randomly distributed at the plasma membrane and not enriched in caveolae. Binding of EGF at 4°C does not change the localization of EGFR,and by immunoelectron microscopy we find that only small amounts of bound EGF localize to caveolae. However, upon patching of lipid rafts, we find...

... content nor cholesterol metabolism changed in Vim -/- cells. Our results are consistent with the view that re-expression of vimentin in epithelial cells could be instrumental to maintain the physical state of rafts and, thus, the function of DRM-associated proteins. Cells were fixed in ice-cold...

.... Brown, D. A. and London, E. ( 1998a ). Functions of lipid rafts in biological membranes. Annu. Rev. Cell Dev. Biol. 14 , 111 -136. Brown, D. A. and London, E. ( 1998b ). Structure and origin of ordered lipid domains in biological membranes. J. Memb. Biol. 164 , 103 -114. Brown, D...