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Keywords: RNA interference
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Journal Articles
J Cell Sci (2015) 128 (13): 2388–2400.
Published: 1 July 2015
... by numerous actin-binding proteins, but regulation of nuclear actin has remained unclear. Here, we performed a genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins that influence either nuclear polymerization or import of actin. We validate 19 factors as specific hits, and show...
Includes: Supplementary data
Journal Articles
J Cell Sci (2011) 124 (9): 1433–1444.
Published: 1 May 2011
... show that 4.1R, the nuclear envelope protein emerin and the intermediate filament protein lamin A/C co-immunoprecipitate, and that 4.1R-specific depletion in human cells by RNA interference produces nuclear dysmorphology and selective mislocalization of proteins from several nuclear subcompartments...
Includes: Supplementary data
Journal Articles
J Cell Sci (2010) 123 (8): 1183–1189.
Published: 15 April 2010
...Monika Dominska; Derek M. Dykxhoorn RNA interference (RNAi)-based technologies offer an attractive strategy for the sequence-specific silencing of disease-causing genes. The application of small interfering (si)RNAs as potential therapeutic agents requires safe and effective methods...
Journal Articles
J Cell Sci (2007) 120 (8): 1317–1323.
Published: 15 April 2007
.... Recently, GW bodies have been linked to RNA interference and demonstrated to be important for short-interfering-RNA- and microRNA-mediated mRNA decay and translational repression. Evidence indicates that both passenger and guide strands of short-interfering RNA duplexes can localize to GW bodies, thereby...
Journal Articles
J Cell Sci (2006) 119 (23): 4944–4951.
Published: 1 December 2006
... substitution in Smu1 that underlies the temperature-sensitive phenotypes of tsTM18 cells. In the present study, we confirmed that Smu1 is associated with the temperature-sensitive defect of tsTM18 by RNA interference. We also found an early temperature effect in DNA synthesis. Because genetic studies...
Journal Articles
J Cell Sci (2006) 119 (5): 846–857.
Published: 1 March 2006
... related to those known in vertebrates, we tested this gene for its implication in protease inhibition by using double-stranded RNA interference (dsRNAi). dsRNAi has proved to be a powerful tool, both to look at gene function and to implement systematic functional screens in contexts not amenable...
Includes: Supplementary data
Journal Articles
J Cell Sci (2005) 118 (23): 5647–5660.
Published: 1 December 2005
..., whereas SV2B is only present on SLM. Neither overexpression nor isoform-specific silencing of SV2A or SV2C by RNA interference modifies depolarization-triggered cytosolic [Ca 2+ ] rises or secretory granule [Ca 2+ ], measured with a VAMP-2 aequorin chimera. This strongly argues against any Ca 2+ transport...
Includes: Supplementary data
Journal Articles
J Cell Sci (2005) 118 (10): 2133–2141.
Published: 15 May 2005
... of the cohesin subunit Scc1 by RNA interference leads to the assembly of chromosomes with severe cohesion defects. A similar yet milder set of defects is observed in cells with reduced levels of Pds5A or Pds5B. In Xenopus egg extracts, mitotic chromosomes assembled in the absence of Pds5A and Pds5B display...
Includes: Supplementary data
Journal Articles
J Cell Sci (2005) 118 (9): 1833–1842.
Published: 1 May 2005
... and ionizing radiation. S2 cells depleted for Grp/DChk1 using RNA interference enter mitosis in the presence of impaired DNA integrity, resulting in prolonged mitosis and mitotic catastrophe. Grp/DChk1 is phosphorylated in a Mei-41/DATR-dependent manner in response to hydroxyurea and ionizing radiation...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2005) 118 (4): 831–841.
Published: 15 February 2005
... and not in the insect procyclic stage. This is the only reported example to date of a differentially expressed ARL1 homologue in any species. We have used RNA interference to demonstrate that ARL1 is essential for viability in T. brucei bloodstream parasites. Prior to cell death, depletion of ARL1 protein...
Includes: Supplementary data
Journal Articles
J Cell Sci (2004) 117 (24): 5905–5912.
Published: 15 November 2004
... was followed for a total of 41 hours after scraping (circles represent control cells and triangles represent PHI-1-knocked-down cells). To investigate the function of endogenous PHI-1, we ablated the production of PHI-1 using RNA interference. Synthetic siRNA duplexes matching different regions...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2004) 117 (21): 4881–4888.
Published: 1 October 2004
... and Drosophila , but in these organisms the process occurs in the absence of DNA methylation. * Author for correspondence (e-mail: jbender@mail.jhmi.edu ) © The Company of Biologists Limited 2004 2004 RNA interference Transposons siRNAs Heterochromatin Cytosine methylation Chromatin...
Journal Articles
J Cell Sci (2004) 117 (20): 4837–4848.
Published: 15 September 2004
... this by inhibiting Rab7 function with RNA interference and overexpression of dominant negative Rab7. We show here that Rab7 was needed for the formation of preferably perinuclear, large aggregates, where the autophagosome marker LC3 colocalised with Rab7 and late endosomal and lysosomal markers. By electron...
Journal Articles
J Cell Sci (2004) 117 (15): 3119–3127.
Published: 1 July 2004
... exocytosis in INS-1E cells. Finally, reduction of the expression of both Syt isoforms by RNA interference did not change basal secretion. Remarkably, hormone release in response to glucose was selectively and strongly reduced, indicating that Syt V and Syt IX are directly involved in the Ca 2+ -dependent...
Journal Articles
J Cell Sci (2003) 116 (14): 2987–2998.
Published: 15 July 2003
...Ana Carvalho; Mar Carmena; Clara Sambade; William C. Earnshaw; Sally P. Wheatley Survivin is an essential chromosomal passenger protein whose function remains unclear. Here, we have used RNA interference to specifically repress Survivin in cultured HeLa cells. Immunoblot analysis showed...
Journal Articles
J Cell Sci (2002) 115 (21): 4053–4059.
Published: 1 November 2002
... proteins were found. In physiological buffers, astrin dimers oligomerized via their globular head domains and formed aster-like structures. Silencing of astrin in HeLa cells by RNA interference resulted in growth arrest, with formation of multipolar and highly disordered spindles. Chromosomes did...
Journal Articles
J Cell Sci (2001) 114 (24): 4557–4565.
Published: 15 December 2001
...Jens Harborth; Sayda M. Elbashir; Kim Bechert; Thomas Tuschl; Klaus Weber We report the first RNAi-induced phenotypes in mammalian cultured cells using RNA interference mediated by duplexes of 21-nt RNAs. The 21 gene products studied have different functions and subcellular localizations. Knockdown...
Journal Articles
J Cell Sci (2001) 114 (17): 3083–3091.
Published: 1 September 2001
...Hervé Vaucheret; Christophe Béclin; Mathilde Fagard Post-transcriptional gene silencing (PTGS) in plants is an RNA-degradation mechanism that shows similarities to RNA interference (RNAi) in animals. Indeed, both involve double-stranded RNA (dsRNA), spread within the organism from a localised...