...? We discuss how this complex can influence cells to proliferate, enter quiescence or differentiate. Centrosome Primary cilium Cell fate Quiescence Differentiation Muscle stem cells Cell cycle RNA University Grants Commission http://dx.doi.org/10.13039/501100001501...

...Zhikai Chen; Shengnan Zheng; Chuanhai Fu ABSTRACT The CRISPR-Cas13d system has a single small effector protein that targets RNA and does not require the presence of a protospacer flanking site in the targeted transcript. These features make CRISPR-Cas13d an attractive system for RNA manipulation...

...Sami Hassine; Florence Bonnet-Magnaval; Louis Philip Benoit Bouvrette; Bellastrid Doran; Mehdi Ghram; Mathieu Bouthillette; Eric Lecuyer; Luc DesGroseillers ABSTRACT Staufen1 (STAU1) is an RNA-binding protein involved in the post-transcriptional regulation of mRNAs. We report that a large fraction...

...Yu Zhan; James S. Dhaliwal; Pauline Adjibade; James Uniacke; Rachid Mazroui; William Zerges The oxidation of biological molecules by reactive oxygen species (ROS) can render them inactive or toxic. This includes the oxidation of RNA, which appears to underlie the detrimental effects of oxidative...

..., and to define their temporal and spatial parameters by using this methodology to label endogenous genes and make reporter constructs that allow specific DNA and RNA regulatory elements to be localized. This Commentary highlights recent reports that have used these techniques to study nuclear organization...

... and an arginine- and glycine-rich domain (RG stretch), which are often found in proteins involved in RNA metabolism, the former being essential for targeting. RNase A treatment dissociates 53BP1 from IR-induced foci. In HeLa cells, dissociation of the M domain without the RG stretch by RNase A treatment can...

... been introduced to optimize the detection of DNA and RNA. The pervasiveness of this technique is largely because of its wide variety of applications and the relative ease of implementation and performance of in situ studies. Although the basic principles of FISH have remained unchanged, high...