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Keywords: PtdIns(4,5)P2
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In collection:
Cilia and Flagella
J Cell Sci (2018) 131 (16): jcs218297.
Published: 17 August 2018
... spectrum . Pediatr. Nephrol.   26 , 1039 - 1056 . 10.1007/s00467-010-1731-7 Wei , H.-C. , Rollins , J. , Fabian , L. , Hayes , M. , Polevoy , G. , Bazinet , C. and Brill , J. A. ( 2008 ). Depletion of plasma membrane PtdIns(4,5)P2 reveals essential roles...
Journal Articles
J Cell Sci (2018) 131 (8): jcs211094.
Published: 13 April 2018
... of these structures. Based on their shape and size, we refer to them as NLIs. Scale bar: 200 nm. The graph shows that PtdIns(4,5)P 2 molecules and NM1 significantly colocalise at the distance of 25–75 nm ( n =21, PCCF>1, ** P ≤0.01). Fig. 1. Nucleoplasmic PtdIns(4,5)P2 appears in small foci which colocalise...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (22): 4047–4056.
Published: 15 November 2015
... , 142 . 10.1038/ncomms1144 D'Angelo , G. , Vicinanza , M. , Di Campli , A. and De Matteis , M. A. ( 2008 ). The multiple roles of PtdIns(4)P - not just the precursor of PtdIns(4,5)P2 . J. Cell Sci.   121 , 1955 - 1963 . 10.1242/jcs.023630 Danson , C. , Brown...
Journal Articles
J Cell Sci (2014) 127 (1): 72–84.
Published: 1 January 2014
... of 10 µM ionomycin. Scale bars: 10 µm. Fig. 1. The presence of PMCA proteins prevents the translocation of the PtdIns(4,5)P2 sensor during ionomycin treatments. The PtdIns(4,5)P2 sensor, PHPLCδ1–RFP was expressed alone or together with GFP–PMCA2wb in sh-HeLa cells; or together with GFP-PMCA4b-LA...
Includes: Supplementary data
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Lipid Biology
J Cell Sci (2013) 126 (16): 3602–3614.
Published: 15 August 2013
.... The concentrations were determined from sum projections of n  = 28 and n  = 79, wild-type and mss4-f12 mutant cells, respectively from two or three independent experiments. Fig. 5. mss4 mutants have reduced levels of PtdIns(4,5)P2, an altered distribution of plasma membrane PtdIns(4,5)P2 and accumulate...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (5): 1247–1259.
Published: 1 March 2013
... and 24 La3+-treated ommatidia). (ii) Ommatidia expressing the PtdIns(4,5)P2-binding PH domain from PLCδ1 (PH–GFP). In control solutions PH–GFP remained localized to the rhabdomeres (r) after red illumination; but in La3+-treated ommatidia (below) it had translocated to the cell body (c), indicating...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (2): 454–463.
Published: 15 January 2013
... on neurosecretion relies on PtdIns(4,5)P2 interaction. (A) PC12 cells were transfected with plasmids encoding GH and Tat or the indicated Tat mutants. 24 hours after transfection, GH secretion was evoked with ATP and quantified. Results are means ± s.e.m. of three experiments performed in triplicate on cells...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (1): 312–326.
Published: 1 January 2013
....   582 , 2102 – 2111 . 10.1016/j.febslet.2008.03.039 Mason   D. , Mallo   G. V. , Terebiznik   M. R. , Payrastre   B. , Finlay   B. B. , Brumell   J. H. , Rameh   L. , Grinstein   S. ( 2007 ). Alteration of epithelial structure and function associated with PtdIns(4,5)P2...
Includes: Supplementary data
Journal Articles
In collection:
Lipid Biology
J Cell Sci (2010) 123 (20): 3535–3546.
Published: 15 October 2010
... for the dominant-negative effect of GFP-PIP5KβE61L. (H) HeLa (left), HEK293 (middle) and N1E-115 (right) cells were transfected with the constructs shown and were labelled with [32P]orthophosphate for 2 hours after which phosphoinositides were extracted and the levels of PtdIns(4,5)P2 were analysed and quantified...
Includes: Supplementary data
Journal Articles
J Cell Sci (2010) 123 (12): 2058–2067.
Published: 15 June 2010
... are in the cytosol-exposed loops of the FERM domain ( Pearson et al., 2000 ). Fig. 1. Drosophila Moe binds to PtdIns(4,5)P2 in a liposome co-sedimentation assay. (A) MoeWT and MoeKN association with phosphatidylcholine liposomes containing increasing concentrations of PtdIns(4,5)P2. The supernatant contains...
Includes: Supplementary data
Journal Articles
J Cell Sci (2010) 123 (9): 1492–1502.
Published: 1 May 2010
... oscillations of plasma-membrane PtdIns(4)P and PtdIns(4,5)P2 levels. The boxed inset shows the part of the recording highlighted by a rectangle on an expanded time base and with the PLCδ1PH-YFP trace inverted to show that the change in PtdIns(4,5)P2 reaches its maximum before that of PtdIns(4)P; n=4. (G) Cross...
Includes: Supplementary data
Journal Articles
Journal Articles
J Cell Sci (2009) 122 (21): 3837–3850.
Published: 1 November 2009
... cadherins and catenins that leads to the activation of PIP5Kα. The resulting PtdIns(4,5)P2 that is produced serves as a substrate for PI3K for the production of PtdIns(3,4,5)P3, and for PLCγ1 for the production of DAG and Ins(1,4,5)P3. This results in an increase in intracellular Ca2+, which, in turn...
Journal Articles
J Cell Sci (2008) 121 (22): 3770–3777.
Published: 15 November 2008
...) and then with bradykinin (1 μM; at t =115 seconds, second arrow). Fig. 3. PLCβ1a and PtdIns(4,5)P2. (A) HeLa cells were stimulated with histamine (100 μM, arrow) after overnight incubation with 1 μM PMA. The red trace depicts the mean normalized fluorescence intensity of GFP-PLCβ1a; the black trace depicts the mean...
Journal Articles
J Cell Sci (2008) 121 (20): 3433–3444.
Published: 15 October 2008
... of point mutations. (A) ENTHR65A/K78A, but not ENTHT107A, exhibits impaired binding to PtdIns(4,5)P2. PtdIns and PtdIns(4,5)P2 (PI and PI(4,5)P2, respectively) were spotted onto nitrocellulose membrane and then incubated with lysate from Dictyostelium cells expressing either wild-type or mutant ENTH:GFP...
Journal Articles
J Cell Sci (2006) 119 (8): 1570–1578.
Published: 15 April 2006
... of the NGF-receptor starts the PtdIns(4,5) P 2 hydrolysis cycle again, and creates a balanced level of free profilin I, which may then contribute to neurite elongation. Where inactive, proteins are shown in grey lettering. Fig. 7. Model for the regulation of profilin-I-actin interaction by PtdIns(4,5)P2...
Journal Articles
J Cell Sci (2004) 117 (17): 3887–3896.
Published: 1 August 2004
... phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2]. This in turn, is hydrolyzed by phospholipase C (PLC; step 4) to form Ins(1,4,5)P3 and DAG in a reaction inhibited by U73122. DAG is then converted to phosphatidic acid (PA) and then CDP-DAG to complete the cycle. Note that WT and LY also inhibit PtdIns 3-kinase...
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Journal Articles