... spectrum . Pediatr. Nephrol.   26 , 1039 - 1056 . 10.1007/s00467-010-1731-7 Wei , H.-C. , Rollins , J. , Fabian , L. , Hayes , M. , Polevoy , G. , Bazinet , C. and Brill , J. A. ( 2008 ). Depletion of plasma membrane PtdIns(4,5)P2 reveals essential roles...

... of these structures. Based on their shape and size, we refer to them as NLIs. Scale bar: 200 nm. The graph shows that PtdIns(4,5)P 2 molecules and NM1 significantly colocalise at the distance of 25–75 nm ( n =21, PCCF>1, ** P ≤0.01). Fig. 1. Nucleoplasmic PtdIns(4,5)P2 appears in small foci which colocalise...

... , 142 . 10.1038/ncomms1144 D'Angelo , G. , Vicinanza , M. , Di Campli , A. and De Matteis , M. A. ( 2008 ). The multiple roles of PtdIns(4)P - not just the precursor of PtdIns(4,5)P2 . J. Cell Sci.   121 , 1955 - 1963 . 10.1242/jcs.023630 Danson , C. , Brown...

... of 10 µM ionomycin. Scale bars: 10 µm. Fig. 1. The presence of PMCA proteins prevents the translocation of the PtdIns(4,5)P2 sensor during ionomycin treatments. The PtdIns(4,5)P2 sensor, PHPLCδ1–RFP was expressed alone or together with GFP–PMCA2wb in sh-HeLa cells; or together with GFP-PMCA4b-LA...

.... The concentrations were determined from sum projections of n  = 28 and n  = 79, wild-type and mss4-f12 mutant cells, respectively from two or three independent experiments. Fig. 5. mss4 mutants have reduced levels of PtdIns(4,5)P2, an altered distribution of plasma membrane PtdIns(4,5)P2 and accumulate...

... and 24 La3+-treated ommatidia). (ii) Ommatidia expressing the PtdIns(4,5)P2-binding PH domain from PLCδ1 (PH–GFP). In control solutions PH–GFP remained localized to the rhabdomeres (r) after red illumination; but in La3+-treated ommatidia (below) it had translocated to the cell body (c), indicating...

... on neurosecretion relies on PtdIns(4,5)P2 interaction. (A) PC12 cells were transfected with plasmids encoding GH and Tat or the indicated Tat mutants. 24 hours after transfection, GH secretion was evoked with ATP and quantified. Results are means ± s.e.m. of three experiments performed in triplicate on cells...

....   582 , 2102 – 2111 . 10.1016/j.febslet.2008.03.039 Mason   D. , Mallo   G. V. , Terebiznik   M. R. , Payrastre   B. , Finlay   B. B. , Brumell   J. H. , Rameh   L. , Grinstein   S. ( 2007 ). Alteration of epithelial structure and function associated with PtdIns(4,5)P2...

... for the dominant-negative effect of GFP-PIP5KβE61L. (H) HeLa (left), HEK293 (middle) and N1E-115 (right) cells were transfected with the constructs shown and were labelled with [32P]orthophosphate for 2 hours after which phosphoinositides were extracted and the levels of PtdIns(4,5)P2 were analysed and quantified...

... are in the cytosol-exposed loops of the FERM domain ( Pearson et al., 2000 ). Fig. 1. Drosophila Moe binds to PtdIns(4,5)P2 in a liposome co-sedimentation assay. (A) MoeWT and MoeKN association with phosphatidylcholine liposomes containing increasing concentrations of PtdIns(4,5)P2. The supernatant contains...

... oscillations of plasma-membrane PtdIns(4)P and PtdIns(4,5)P2 levels. The boxed inset shows the part of the recording highlighted by a rectangle on an expanded time base and with the PLCδ1PH-YFP trace inverted to show that the change in PtdIns(4,5)P2 reaches its maximum before that of PtdIns(4)P; n=4. (G) Cross...

... cadherins and catenins that leads to the activation of PIP5Kα. The resulting PtdIns(4,5)P2 that is produced serves as a substrate for PI3K for the production of PtdIns(3,4,5)P3, and for PLCγ1 for the production of DAG and Ins(1,4,5)P3. This results in an increase in intracellular Ca2+, which, in turn...

...) and then with bradykinin (1 μM; at t =115 seconds, second arrow). Fig. 3. PLCβ1a and PtdIns(4,5)P2. (A) HeLa cells were stimulated with histamine (100 μM, arrow) after overnight incubation with 1 μM PMA. The red trace depicts the mean normalized fluorescence intensity of GFP-PLCβ1a; the black trace depicts the mean...

... of point mutations. (A) ENTHR65A/K78A, but not ENTHT107A, exhibits impaired binding to PtdIns(4,5)P2. PtdIns and PtdIns(4,5)P2 (PI and PI(4,5)P2, respectively) were spotted onto nitrocellulose membrane and then incubated with lysate from Dictyostelium cells expressing either wild-type or mutant ENTH:GFP...

... of the NGF-receptor starts the PtdIns(4,5) P 2 hydrolysis cycle again, and creates a balanced level of free profilin I, which may then contribute to neurite elongation. Where inactive, proteins are shown in grey lettering. Fig. 7. Model for the regulation of profilin-I-actin interaction by PtdIns(4,5)P2...

... phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2]. This in turn, is hydrolyzed by phospholipase C (PLC; step 4) to form Ins(1,4,5)P3 and DAG in a reaction inhibited by U73122. DAG is then converted to phosphatidic acid (PA) and then CDP-DAG to complete the cycle. Note that WT and LY also inhibit PtdIns 3-kinase...