... in buffer and behaviour recorded. Composite images are shown in the top rows. GFP and RFP/TRITC channels are shown in the rows beneath. Numbers indicate time in seconds. Scale bars: 10 µm. Fig. 1. PtdIns(3,4,5)P3 (PIP3)-labelled structures in growing cells. (A) Phagocytosis: a Dictyostelium cell takes up...

... (hr:min). Scale bars: 20 µm. Fig. 1. Constitutive synthesis of PtdIns(3,4,5)P3 during meiotic maturation of mouse oocytes. GV oocytes were injected with mRNAs encoding PH-Akt–GFP, EGFP–Lifeact and EGFP–Fmn2 and imaged by time-lapse confocal microscopy during meiotic maturation to document...

...–Myo10TailΔPH2 to PtdIns(3,4,5) P 3 in a lipid pull-down assay. Comparison by Student's t -test (* P <0.05). Graphs show means + s.e.m. of three independent experiments. Fig. 2. Myo10 PH2 domain binds specifically to PtdIns(3,4,5)P3. (A) Constructs allowing the expression of different domains of Myo10...

... in a macrophage expressing YFP-BtkPH and CFP-MEM. Images were collected every 3 seconds; 6-second intervals are depicted. PtdIns(3,4,5) P 3 concentrations in the plasma membrane began to increase shortly after ruffle closure (66 sec). Scale bar: 1 μm. Fig. 3. PtdIns(3,4,5)P3 dynamics during macropinosome...

... attenuated chemotaxis defects of sodC– cells. (A) Cells expressing the PtdIns(3,4,5)P3 marker GFP-PHcrac were pulsed for 4 hours with 50 nM cAMP, and either left in DB buffer or treated with 15 μM or 50 μM LY294002 (LY) for 20 minutes. GFP-PHcrac aberrantly localized at the plasma membrane of sodC– cells...