...: The Cdc42 guanine-nucleotide-exchange factor Cdc24 interacts with septins during bud site assembly. This interaction assists in recruiting septins and stabilizing Cdc24 at the incipient bud site. Cell polarity Protein–protein interaction SPLIFF Positive feedback Split-ubiquitin Yeast Cdc42...

... and integrity in organisms that are distantly related, suggesting they have a conserved role in mTORC2 assembly and functioning. Mammalian target of rapamycin mTOR AKT PKB Dictyostelium Protein–protein interaction Rictor Mammalian, or mechanistic, target of rapamycin (mTOR) is a serine...

... of GTPase activation based on tripartite split-GFP that enables the evaluation of GEF activity and the effect of modulators of GTPase activation in a high-content analysis. GTPase activation Split-GFP Protein–protein interaction GEF Guanine nucleotide exchange factor Cell-based assays High...

... ubiquitylation and degradation via ER-related degradation. The E3 ligase HUWE1 is required and adds K11-linked ubiquitin chains to ENaC prompting its degradation. Epithelial Na + channel Derlin-1 Ubiquitylation Protein–protein interaction Ubiquitin-mediated proteasome degradation Hypertension...

... luminal domain is the minimal requirement to form the sarcoplasmic reticulum Ca 2+ sensor, which governs cardiac Ca 2+ homeostasis and reliable heartbeat. Ca 2+ intracellular release Calsequestrin Excitation-contraction coupling Luminal Ca 2+ sensor Protein–protein interaction Ryanodine...

... downstream factors. FoxO1 inhibits basal transcription and gonadotropin-releasing hormone (GnRH)-mediated induction of the gene encoding luteinizing hormone β ( Lhb ) in pituitary gonadotrope cells. This response is likely to occur through protein–protein interactions between the FoxO1 DNA-binding domain...

... proteome analysis to determine LPPR1-interacting proteins and identifies a functional cooperation between LPPR family members. Membrane protrusion Protein-protein interaction Filopodia Actin The lipid phosphate phosphatase-related proteins (LPPRs), also known as plasticity-related genes...

... . 10.1074/mcp.M900430-MCP200 Lin , X. , Li , X. , Jiang , M. , Chen , L. , Xu , C. , Zhang , W. , Zhao , H. , Sun , B. , Xu , X. , Nan , F. , et al. ( 2012 ). An activity-based probe reveals dynamic protein–protein interactions mediating IGF-1R...

... and RFP fusion proteins for immunoprecipitation with anti-GFP nanobody, protein–protein interactions could be reproducibly visualized by directly observing the immunoprecipitates under a microscope, and evaluated using a microplate reader, without requiring immunoblotting. Using this ‘visible...

... of the daughter cell at the end of cytokinesis ( Barr and Gruneberg, 2007 ; Onishi et al., 2013 ). Cytokinesis IQGAP F-BAR protein Protein–protein interaction Iqg1p is synthesized late during the cell cycle when its interaction with Mlc1p targets the protein immediately to the bud neck ( Fig...

... ( nils.johnsson@uni-ulm.de ) 9 9 2010 © 2011. 2011 Constraint interaction network Protein–protein interaction Organelle inheritance Polarized growth Protein kinase Protein phosphatase Systems biology Inactivation of proteins that participate in more than one cellular process leads...

... for the development of effective therapeutic agents. * Author for correspondence (e-mail: sdedhar@interchange.ubc.ca ) 7 8 2008 © The Company of Biologists Limited 2008 2008 Cancer Development Extracellular matrix Integrin-linked kinase Protein-protein interaction Signal transduction...

...-protein interaction Telomeres are the ends of linear eukaryotic chromosomes. Their main function is to maintain genome integrity by replicating chromosome ends and protecting them from exonucleolytic degradation and mutual fusions (reviewed in Blackburn, 2001 ). Telomeres also have a role...

... and biochemical studies have revealed how β-catenin engages in critical protein-protein interactions by using its armadillo repeat region and its N- and C-terminal domains. The groove in the armadillo repeat region is a particularly interesting feature of β-catenin, since it serves as a common binding site...

.... This effect is associated with specific intracellular protein-protein interaction and the formation of a sG i2 –D 2 -receptor complex. It has been suggested that the formation of this complex serves to prevent D 2 receptors from reaching the cell membrane. Treatment of D 2 -receptor-expressing cells...

... in protein-protein interaction (e.g. stabilizing the protein interaction). It is of note that overexpression of a deletion mutant of SCAMP1 lacking the N-terminal domain potently inhibited transferrin uptake by endocytosis ( Fernandez-Chacon et al., 2000 ). Longer isoforms and shorter isoforms of SCAMPs may...

... the same modular organisation of mammalian Fe65s, including the WW, PTB1 and PTB2 domains, confirming that the three protein-protein interaction domains constitute the main functional regions of these molecular adaptors. The PTB2 domain of FEH-1 is also functionally related to the corresponding domains...