... roles are poorly characterized. Here, we identify a surprising new mechanism for its role in electrolyte homeostasis. mTOR mTORC2 Rictor SGK1 WNK1 WNK kinase ENaC Electrolytes Ion transport K + homeostasis Kidney epithelial cells Protein–protein interaction National Institutes...

... and stabilizing Cdc24 at the incipient bud site. Cell polarity Protein–protein interaction SPLIFF Positive feedback Split-ubiquitin Yeast Cdc42 Deutsche Forschungsgemeinschaft 10.13039/501100001659 Jo 187/5-2 Yeast cells grow and divide by forming a bud at the beginning of each...

... related, suggesting they have a conserved role in mTORC2 assembly and functioning. Mammalian target of rapamycin mTOR AKT PKB Dictyostelium Protein–protein interaction Rictor Compagnia di San Paolo 10.13039/100007388 12-CSP-C03-065 Università degli Studi di Torino...

... in a high-content analysis. GTPase activation Split-GFP Protein–protein interaction GEF Guanine nucleotide exchange factor Cell-based assays High-content analysis La Ligue Régionale contre le Cancer Institut National de la Santé et de la Recherche Médicale Small...

... ubiquitylation and degradation via ER-related degradation. The E3 ligase HUWE1 is required and adds K11-linked ubiquitin chains to ENaC prompting its degradation. Epithelial Na + channel Derlin-1 Ubiquitylation Protein–protein interaction Ubiquitin-mediated proteasome degradation National...

.... Invest.   101 , 1385 - 1393 . 10.1172/JCI1362 Kobayashi , Y. , Alseikhan , B. and Jones , L. ( 2000 ). Localization and characterization of the calsequestrin-binding domain of triadin 1. Evidence for a charged beta-strand in mediating the protein-protein interaction . J. Biol...

..., and FoxO1 phosphorylation promotes ENaC membrane trafficking by binding to 14-3-3ε in epithelium. Epithelial Na + channel FoxO1 14-3-3ε Protein–protein interaction Protein phosphorylation The epithelial Na + channel (ENaC) localizes to the apical membranes of principal cells...

... a functional cooperation between LPPR family members. Membrane protrusion Protein-protein interaction Filopodia Actin The lipid phosphate phosphatase-related proteins (LPPRs), also known as plasticity-related genes (PRGs), are a family of six-transmembrane proteins that are enriched in brain...

... induces Rap1 activation to promote its own surface expression by stimulating receptor recycling, thereby providing a potential explanation for the long-lasting action of GABA. GABA B receptor Rap1 Protein–protein interaction Internalization Recycling For many GPCRs, signal transduction...

... and RFP fusion proteins for immunoprecipitation with anti-GFP nanobody, protein–protein interactions could be reproducibly visualized by directly observing the immunoprecipitates under a microscope, and evaluated using a microplate reader, without requiring immunoblotting. Using this ‘visible...

... ( [email protected] ) Competing interests The authors declare no competing interests. 17 03 2014 8 05 2014 © 2014. Published by The Company of Biologists Ltd 2014 Cytokinesis IQGAP F-BAR protein Protein–protein interaction In the budding yeast Saccharomyces...

... a subset of the observed phenotypes and assigns the Ptc1p–Nbp2p module a role in synchronizing the associated kinases during the cell cycle. Constraint interaction network Protein–protein interaction Organelle inheritance Polarized growth Protein kinase Protein phosphatase Systems biology...

... for the development of effective therapeutic agents. * Author for correspondence (e-mail: [email protected] ) 7 8 2008 © The Company of Biologists Limited 2008 2008 Cancer Development Extracellular matrix Integrin-linked kinase Protein-protein interaction Signal transduction...

... (misspl.) at intron 5 and intron 7 corresponding to alternative splicing variants d and f (see Fig. 5 ) are shown * Author for correspondence (e-mail: [email protected] ) 21 8 2007 © The Company of Biologists Limited 2007 2007 Telomere Protein-protein interaction...

... and biochemical studies have revealed how β-catenin engages in critical protein-protein interactions by using its armadillo repeat region and its N- and C-terminal domains. The groove in the armadillo repeat region is a particularly interesting feature of β-catenin, since it serves as a common binding site...

.... This effect is associated with specific intracellular protein-protein interaction and the formation of a sG i2 –D 2 -receptor complex. It has been suggested that the formation of this complex serves to prevent D 2 receptors from reaching the cell membrane. Treatment of D 2 -receptor-expressing cells...

..., pH environment within the immature granule appears to play a crucial role in retaining regulated secretory proteins within the granule, whereas other proteins are diverted to the constitutive secretory pathway ( Arvan and Castle, 1998 ). In addition, protein-protein interactions as well as membrane...

...-protein interaction domains constitute the main functional regions of these molecular adaptors. The PTB2 domain of FEH-1 is also functionally related to the corresponding domains of the mammalian homolog proteins, as it is able to interact with the cytosolic domain of APL-1. Furthermore, the high...

... by Company of Biologists 2001 Protein-protein interaction Modular binding domains Signal transduction The idea that specific protein-protein interactions govern many biological processes is by now so deeply ingrained that it is easy to forget that it has only been a decade or so since...

...)83013-3 Bartel , P. L. and Fields , S. ( 1995 ). Analyzing protein-protein interactions using the two-hybrid system . Meth. Enzymol . 254 , 241 – 263 . 10.1016/0076-6879(95)54018-0 Becker , T. S. , Burgess , S. M. , Amsterdam , A. H. , Allende , M. L...