... increase in cellular cAMP and consequent responses in the cytoplasm required for cell–cell fusion. Here, we identify a new participant in ciliary signaling, Gamete-Specific Protein Kinase (GSPK). GSPK is essential for the adhesion-induced cAMP increase and for rapid gamete fusion. The protein...

... regulator of new end growth and demonstrated that Fic1, a cytokinetic factor, is required for normal polarized growth at new ends. Here, we report that Fic1 is phosphorylated on two C-terminal residues, which are each targeted by multiple protein kinases. Endogenously expressed Fic1 phosphomutants cannot...

...Margot Riggi; Beata Kusmider; Robbie Loewith ABSTRACT Target of rapamycin (TOR) is a serine/threonine protein kinase conserved in most eukaryote organisms. TOR assembles into two multiprotein complexes (TORC1 and TORC2), which function as regulators of cellular growth and homeostasis by serving...

... by a signaling cascade that includes EGFR, phospholipase C γ1 (PLCγ1), protein kinase C ε (PKCε), and NF‐κB. Activation of EGFR results in the activation of PLCγ1 and the subsequent production of diacylglycerol; this in turn activates PKCε, which is then monoubiquitylated by the E3 ligase RINCK1 (also known...

...Ivo Zemp; Franziska Wandrey; Sanjana Rao; Caroline Ashiono; Emanuel Wyler; Christian Montellese; Ulrike Kutay ABSTRACT Biogenesis of 40S pre-ribosomal subunits requires many trans-acting factors, among them several protein kinases. In this study, we show that the human casein kinase 1 (CK1...

... a subset of the observed phenotypes and assigns the Ptc1p–Nbp2p module a role in synchronizing the associated kinases during the cell cycle. Constraint interaction network Protein–protein interaction Organelle inheritance Polarized growth Protein kinase Protein phosphatase Systems biology...

...Barry J. Collins; Maria Deak; Vicky Murray-Tait; Kate G. Storey; Dario R. Alessi AGC kinases are mediators of signalling responses stimulated by agonists and are activated following phosphorylation at their T-loop residue by the 3-phosphoinositide-dependent protein kinase-1 (PDK1). Agonists...

...Krysten J. Palmer; Joanne E. Konkel; David J. Stephens The export of secretory cargo from the endoplasmic reticulum is mediated by the COPII complex. In common with other aspects of intracellular transport, this step is regulated by protein kinase signalling. Recruitment of the COPII complex...

...Jennifer L. Morrell; Connie B. Nichols; Kathleen L. Gould Two relatives of the GIN4 protein kinase family, Cdr1p and Cdr2p, exist in the yeast Schizosaccharomyces pombe. Although in Saccharomyces cerevisiae GIN4-related kinases influence septin ring organization and septin rings influence...

...Christine Latif; Nicole R. den Elzen; Matthew J. O'Connell The G2 DNA damage checkpoint prevents mitotic entry in the presence of DNA damage. This requires the activation of the phosphoinositide-3-kinase-related protein kinases ATR and ATM in human cells and the ATR homologue Rad3 in the fission...

...Stephan Bek; Rolf Kemler β-Catenin is a multi-functional cellular component and a substrate for several protein kinases. Here we investigated the interaction of protein kinase CKII (casein kinase II) and β-catenin. We show that CKII phosphorylates the N-terminal region of β-catenin and we...

...Lorenzo A. Pinna CK2 is an extremely conserved pleiotropic protein kinase with a growing list of more than 300 substrates, the majority of which are proteins implicated in signal transduction, gene expression and other nuclear functions. The CK2 phosphoacceptor sites are specified by multiple...

... to realise that Cdc2 and cyclins form a stoichiometric complex and that a cyclin subunit is necessary for the Cdc2 subunit to gain its protein kinase activity. Cyclins were first recognized as proteins whose abundance oscillates during the early cell cycles of marine invertebrate eggs and their connection...

[email protected] ) 22 5 2001 © The Company of Biologists Limited 2001 2001 Endoplasmic reticulum Protein kinase Ribonuclease RNA Processing Signal transduction Cells respond to the accumulation of malfolded proteins in the endoplasmic reticulum (ER) by activating signaling pathways...

.... Forsburg, personal communication). Nonetheless, we still know very little about the role of Cdc7p-Dbf4p after DNA replication in meiosis. As in the cases of most protein kinases, ‘upstream’ events (kinase regulation) and ‘downstream’ events (kinase substrates) involving Cdc7p can be studied. Amazing...

... and associating with a protein kinase. Using in vitro transcription and translation, we now demonstrate that the nucleic acid binding function maps to the carboxy-terminal domain of the molecule, a region rich in arginine-glycine-glycine motifs. Our experiments reveal that a central region containing a high...

...Stefan D. Gross; Calvin Simerly; Gerald Schatten; Richard A. Anderson ABSTRACT Casein kinase I is a family of serine/threonine protein kinases common to all eukaryotes. In yeast, casein kinase I homologues have been linked to the regulation of growth, DNA repair and cell division. In addition...

... (cytoplasmic domain) was subjected to in vitro phosphorylation. Occludin was phos-phorylated on serine and threonine residues by protein kinase CK2 and p34 cdc2 /cyclin B complex, but was not sig-nificantly phosphorylated by mitogen-activated protein kinase, protein kinase CK1 and p38 Syk tyrosine kinase. We...

...Lothar Goretzki; Barbara M. Mueller ABSTRACT Internalization of the urokinase-type plasminogen activator (uPA) requires two receptors, the uPA receptor (uPAR) and the low density lipoprotein receptor-related protein (LRP)/α 2 -macroglobulin (α 2 M) receptor. Here, we address whether protein kinases...

... expression libraries to isolate cDNAs encoding these putative centrosomal microtubule-associated proteins. Here we report the cloning of one such cDNA that encodes a novel serine/threonine protein kinase called LK6. The protein appears to exist in two forms: an abundant 185 kDa form and a rarer ∼220 kDa form...