.... In this Review, we provide a brief overview of the structure and functions of the actin-monomer-binding protein profilin-1 (Pfn1) and then discuss how dysregulated expression of Pfn1 contributes to diseases associated with the cardiovascular system. Summary: This Review discusses the role of actin-binding...

... previously suggested that actin filaments form macromolecular complexes with plasma membrane-spanning adhesins to generate force during migration. Mutations in the actin-binding region of profilin, a near ubiquitous actin-binding protein, revealed that loss of actin binding also correlates with loss of force...

... profound effects on cellular behavior. This Review focuses on the potential factors that could create functionally distinct pools of actin monomers in the cell, including differences between the actin isoforms and the regulation of G-actin by monomer binding proteins, such as profilin and thymosin β4...

... barbed-end-interacting proteins and the kinetic mechanisms by which they regulate actin filament assembly. We take F-actin capping protein, gelsolin, profilin and barbed-end-tracking polymerases, including formins and WH2-domain-containing proteins, as examples, and illustrate how their activity...

...Enni Bertling; Omar Quintero-Monzon; Pieta K. Mattila; Bruce L. Goode; Pekka Lappalainen Profilin and cyclase-associated protein (CAP, known in yeast as Srv2) are ubiquitous and abundant actin monomer-binding proteins. Profilin catalyses the nucleotide exchange on actin monomers and promotes...

... μm. * Author for correspondence (e-mail: [email protected] ) 21 11 2005 © The Company of Biologists Limited 2006 2006 Stress fiber Focal adhesion IgC2 α-actinin VASP Profilin Myopalladin Nebulin The ability to move in response to a stimulus...

... © The Company of Biologists Limited 2003 2003 Cellular slime mold Actin Culmination Slug Morphogenesis Profilin Proper spatial and temporal regulation of cytoskeletal function is essential for such eukaryotic cell activities as mitosis, endocytosis,exocytosis, cell migration...

...David R. Kovar; Pinfen Yang; Winfield S. Sale; Bjørn K. Drobak; Christopher J. Staiger We report the characterization of a profilin orthologue from Chlamydomonas reinhardtii . CrPRF, probably the only profilin isoform, is present in both the cell body and flagella. Examination of vegetative...

... was required for the remodelling of the actin cytoskeleton, extension of pseudopodia and efficient particle internalisation. We also report that SLP-76, Vav and profilin were recruited to forming phagosomes. Upon induction of phagocytosis, a large molecular complex, consisting in part of Ena/VASP proteins...

... with different ligands: the FH1 domain for binding the actin-associated protein profilin; the RBD for targeting activated Rho; and the C-terminal CIID module for autoregulation of the overall diaphanous activity. Using deletion constructs of the murine mDia1, we have analyzed the functional properties...

...Marcus Geese; Kathrin Schlüter; Martin Rothkegel; Brigitte M. Jockusch; Jürgen Wehland; Antonio S. Sechi ABSTRACT The spatial and temporal activity of the actin cytoskeleton is precisely regulated during cell motility by several microfilament-associated proteins of which profilin plays an essential...

...K. Schlüter; M. Schleicher; B. M. Jockusch ABSTRACT For a detailed analysis of the profilin-actin interaction, we designed several point mutations in bovine profilin I by computer modeling. The recombinant proteins were analyzed in vitro for their actin-binding properties. Mutant proteins...

... in the mature ascus. Treatment with the actin depolymerising drug Latrunculin A showed that F-actin is required for cell fusion and spore formation. Latrunculin A treatment extended all stages from karyogamy to meiosis I. The S. pombe homologue of the actin binding protein profilin, Cdc3, was shown...

...Michael Haugwitz; Angelika A. Noegel; Daniela Rieger; Friedrich Lottspeich; Michael Schleicher ABSTRACT Two profilin isoforms (profilins I and II) have been purified from Dictyostelium discoideum , using affinity chromatography on a poly(L-proline) matrix; the isoforms could be separated by cation...