... fibrosis, ΔF508, is misfolded and retained by ER quality control. As oligosaccharide moieties of glycoproteins are known to mediate interactions with ER lectin chaperones, we investigated the role of N-linked glycosylation in the processing of wild-type and ΔF508 CFTR. We found that N-glycosylation and ER...

... is synthesized as a 65 kDa non-active precursor that subsequently undergoes proteolytic cleavage, yielding 8 kDa and 50 kDa protein subunits that heterodimerize to form an active enzyme. The protease responsible for heparanase processing is currently unknown, as is the sub-cellular processing site. In this study...

... proteins was performed by anti-FLAG antibody chromatography. FLAG-tagged proteins from cell lysates were immobilized to anti-FLAG M2-conjugated agarose beads( Fig. 6A,B , lanes 2). Silver-stained SDS gels revealed that mainly the diglycosylated PrP form was purified by this one-step purification process...

...Liat Nadav; Amiram Eldor; Oron Yacoby-Zeevi; Eli Zamir; Iris Pecker; Neta Ilan; Benjamin Geiger; Israel Vlodavsky; Ben-Zion Katz Heparanase is a heparan-sulfate-degrading endoglycosidase that has important roles in various biological processes, including angiogenesis, wound healing and metastatsis...

... and during storage in the micronemes. TgMIC6 is processed at the N-terminus, probably in the trans-Golgi network, and the cleavage site has been precisely mapped. Additionally, like other members of the thrombospondin-related anonymous protein family, TgMIC2, TgMIC6 and TgMIC8 are proteolytically cleaved...

..., the apical membrane of CF airway epithelia is Cl − -impermeable, a defect that probably contributes to the pathogenesis of the disease. © The Company of Biologists Limited 1993 1993 cystic fibrosis chloride channel processing trafficking apical membrane Journal of Cell Science, Supplement 17...