...Ilya Chuykin; Herbert Schulz; Kaomei Guan; Michael Bader Summary During early mammalian development, primitive endoderm (PrE) is specified and segregated away from the pluripotent epiblast. At a later developmental stage, PrE forms motile parietal endoderm (PE) lying proximal to the trophectoderm...

...Eloi Montanez; Esra Karaköse; Denise Tischner; Andreas Villunger; Reinhard Fässler Summary The focal adhesion (FA) protein PINCH-1 is required for the survival of primitive endoderm (PrE) cells. How PINCH-1 regulates this fundamental process is not known. Here, we use embryoid bodies (EBs...

... of β-catenin, functioning post-transcriptionally upon CTNNB1 mRNA stability. * Author for correspondence ( [email protected] ) 2 2 2010 © 2010. 2010 Wnt β-catenin Frizzled Dvl KSRP RNA decay Canonical pathway Lef/Tcf Primitive endoderm K-Homology splicing...

... of p38 MAPK is critical for canonical Wnt–β-catenin signaling. Chemical inhibitors of p38 MAPK (SB203580 or SB239063) and expression of a dominant negative-version of p38 MAPK attenuate Wnt3a-induced accumulation of β-catenin, Lef/Tcf-sensitive gene activation, and primitive endoderm formation...

...Takashi Hamazaki; Masahiro Oka; Shinya Yamanaka; Naohiro Terada When embryonic stem cells are allowed to aggregate, the outer layer of the aggregated spheres (referred to as embryoid bodies) differentiates into primitive endoderm. This initial specification of cell lineage facilitates further...