... the structure of primary cilia, evidence for environmental stimuli affecting primary cilia structures is limited. Here, we investigated an extracellular stress that affected primary cilia morphology and its underlying mechanisms. Hyperosmotic shock induced reversible shortening and disassembly of the primary...

...Sanja Sviben; Alexander J. Polino; Isabella L. Melena; Jing W. Hughes ABSTRACT The definitive demonstration of protein localization on primary cilia has been a challenge for cilia biologists. Primary cilia are solitary thread-like projections that have a specialized protein composition...

...Hiroshi Hanafusa; Shin Kedashiro; Mako Gotoh; Ko-hei Saitoh; Hironori Inaba; Tomoki Nishioka; Kozo Kaibuchi; Masaki Inagaki; Naoki Hisamoto; Kunihiro Matsumoto ABSTRACT Primary cilia are antenna-like organelles that regulate growth and development via extracellular signals. However, the molecular...

...Tetsuo Kobayashi; Tatsuya Ikeda; Reo Ota; Takafumi Yasukawa; Hiroshi Itoh ABSTRACT Primary cilia are near-ubiquitously assembled on cells in the human body, and are broadly associated with genetic diseases and cancers. In the early stage of ciliogenesis, the ciliary vesicle (CV) is formed...

... to the base of the primary cilium, but is not a cargo of KIF13B itself. Consistent with a ciliary function for Ap80, its depletion led to elongated primary cilia and reduced agonist-induced ciliary accumulation of SMO, a key component of the Hedgehog signaling pathway, whereas Ap80 overexpression caused...

... or patient group ( Fig. 3 B, lower gene cluster indicated by red bar). These processes were either involved in cell proliferation or in primary cilium formation ( Fig. 3 D; Table S5 ). Given that it is known that primary cilia are required for SHH signaling, it seemed paradoxical that younger patients have...

..., which forms an ion channel in primary cilia and endoplasmic reticulum (ER) membranes of renal collecting duct cells. Elevated internal Ca 2+ modulates polycystin-2 voltage-dependent gating and subsequent desensitization – two biophysical regulatory mechanisms that control its function at physiological...

... by The Company of Biologists Ltd 2020 Summary: Using LUHMES, a ciliated human neuronal cell model, the underlying ‘neurobiology’ of cilia and ciliopathies can be investigated along a complete time line of neuron differentiation. Primary cilia Neuronal cilia SHH signaling Neuronal differentiation...

... and homeostasis, limiting the potential for these pathways to be targeted therapeutically. Primary cilia are subcellular regulators of cellular signalling, controlling how signalling is organized, encoded and, in some instances, driving or influencing pathogenesis. Our previous research revealed that disruption...

... Eggenschwiler , J. T. and Anderson , K. V. ( 2007 ). Cilia and developmental signaling . Annu. Rev. Cell Dev. Biol.   23 , 345 - 373 . 10.1146/annurev.cellbio.23.090506.123249 Fu , W. , Asp , P. , Canter , B. and Dynlacht , B. D. ( 2014 ). Primary cilia control hedgehog...

... in the organelle. Certain G-protein-coupled receptors (GPCRs) specifically localize to the membrane compartment of primary cilia. To gain insight into the mechanisms that regulate ciliary GPCR sorting, we investigated the atypical small GTPase RAB-like 2 (RABL2; herein referring to the near-identical human...

...Pratim Chowdhury; Reid T. Powell; Clifford Stephan; Ivan P. Uray; Tia Talley; Menuka Karki; Durga Nand Tripathi; Yong Sung Park; Michael A. Mancini; Peter Davies; Ruhee Dere ABSTRACT Loss of the gene von Hippel–Lindau ( VHL ) is associated with loss of primary cilia and is causally linked...

...Tomoaki Nagai; Sachiho Mukoyama; Harumi Kagiwada; Naoki Goshima; Kensaku Mizuno ABSTRACT Primary cilia are antenna-like sensory organelles that transmit various extracellular signals. Ciliogenesis requires the removal of CP110 and its interactor CEP97 from the mother centriole for initiating...

...Emily R. Moore; Yuchen Yang; Christopher R. Jacobs ABSTRACT Although Prx1 (also known as PRRX1)-expressing cells and their primary cilia are critical for embryonic development, they have yet to be studied in the context of postnatal skeletogenesis owing to the lethality of mouse models. A tamoxifen...

... . 10.1038/nrm3117 Dorn , K. V. , Hughes , C. E. and Rohatgi , R. ( 2012 ). A Smoothened-Evc2 complex transduces the Hedgehog signal at primary cilia . Dev. Cell   23 , 823 - 835 . 10.1016/j.devcel.2012.07.004 Duldulao , N. A. , Lee , S. and Sun , Z. ( 2009...

.... Interestingly, these were further associated with reduced ciliary smoothened (Smo) levels which could be rescued by expression of wild-type, but not 5-phosphatase-dead INPP5E ( Dyson et al., 2017 ), suggesting a ciliary gatekeeper role for INPP5E, and possibly OCRL, in the transition zone of primary cilia...

...Brian S. Nielsen; Raj R. Malinda; Fabian M. Schmid; Stine F. Pedersen; Søren T. Christensen; Lotte B. Pedersen ABSTRACT Primary cilia are microtubule-based sensory organelles projecting from most quiescent mammalian cells, which disassemble in cells cultured in serum-deprived conditions upon re...

... al., 2007 ). However, other examples of ciliary proteins functioning in cGMP signaling are mostly lacking. Primary cilia are elaborate cellular organelles present on the surfaces of a large proportion of animal cells, including neurons, and function as sensory devices specialized for transducing...

... interests The authors declare no competing interests. 26 07 2013 28 12 2013 © 2014. Published by The Company of Biologists Ltd 2014 Ciliary localization Embryonic stem cell Floxin genetic engineering Gli transcription factors Hedgehog signaling Primary cilia Hedgehog...

... moved along linear paths into and out of developing lamellipodia, indicating vesicular trafficking of NHE1–GFP. The transfected MEFs were grown to confluency and serum starved for 24 h to form primary cilia. A scratch was made, PDGF-AA was added alone or with one of the inhibitors, and the cells...