... to recruit other centrosomal proteins. In Caenorhabditis elegans , the scaffold protein SPD-5 forms a PCM scaffold in a polo-like kinase 1 (PLK-1) phosphorylation-dependent manner. However, how phosphorylation of SPD-5 promotes PCM scaffold assembly is unclear. Here, we identified three functional domains...

...Kieu T. M. Pham; Qing Zhou; Yasuhiro Kurasawa; Ziyin Li ABSTRACT Trypanosoma brucei possesses a motile flagellum that determines cell morphology and the cell division plane. Inheritance of the newly assembled flagellum during the cell cycle is controlled by the Polo-like kinase homolog TbPLK, which...

... kinetoplastid-specific cytokinetic protein, identifies the function of individual domains and implicates the protein in the positioning and timing of T. brucei cleavage furrow ingression. Trypanosoma brucei Cleavage furrow ingression Cytokinesis Polo-like kinase Cytoskeleton Flagellum attachment...

... element proteins is required for meiotic prophase exit. Meiosis Synaptonemal complex G2/MI transition Polo-like kinase Spermatocyte Meiosis, a defining event of gametogenesis, is a specialized cell division involving one round of chromosome replication followed by two rounds of chromosome...

...Zhonglian Yu; Yi Liu; Ziyin Li Polo-like kinases (Plks) play multiple roles in mitosis and cytokinesis in eukaryotes and are characterized by the C-terminal Polo-box domain (PBD), which is implicated in binding to Plk substrates, targeting Plk and regulating Plk activity. The Plk homolog...

... overduplication and the formation of multipolar spindles. By contrast, the Polo-like kinase Cdc5 is necessary for SPB duplication and interacts antagonistically with Ipl1 at the meiotic SPB to ensure proper SPB separation. Our data suggest that Ipl1 coordinates SPB dynamics with the two chromosome segregation...