.../ Highlighted Article: The vesicle-tethering and fusion factors Boi1 and Boi2 are shown to initiate the formation of actin filaments by recruiting the Bni1–Bud6 actin nucleation complex to sites of exocytosis. Polar growth Vesicular traffic Actin nucleation Cdc42 Split-Ubiquitin Yeast...

... subunits of the exocyst, Cdc42p and the module for generating active Cdc42p identify Boi1p and Boi2p as essential mediators between exocytosis and polar growth. The PH domains of Boi1/2p carry the essential activities of the proteins and target them to the cell tip (Hallett et al., 2002). Mutations in PH...

... the observed higher eisosome density at the cortex of germ bubbles. As in Saccharomyces cerevisiae , eisosomes in A. gossypii are very stable. In contrast to S. cerevisiae , however, the A. gossypii homologue of Pil1, one of the main eisosome subunits, is very important for polar growth, whereas the homologue...

...Michael Köhli; Virginie Galati; Kamila Boudier; Robert W. Roberson; Peter Philippsen We use the fungus Ashbya gossypii to investigate how its polar growth machinery is organized to achieve sustained hyphal growth. In slowly elongating hyphae exocyst, cell polarity and polarisome proteins...

... Bem1 in polarity maintenance in U. maydis . We found that Bem1 and Cdc24 physically interact and colocalize at cell tips and that Cdk5 regulates this interaction. Our data suggest a method by which Cdk5 could regulate polar growth in this phytopathogenic fungus. Cdc24 is an essential protein in U...

...Klaus Leonhard; Paul Nurse Polar growth is a crucial process during cell morphogenesis. The microtubule and actin cytoskeletons, and vesicular transport are tightly regulated to direct cellular growth and to generate specific cell forms. We demonstrate here that the Ste20-related protein kinase...

... (fluorescence) time-lapse microscopy. Loss of WAL1 led to slow growth and defects in polarized growth that produced swellings in subapical regions, whereas formation of hyphal tips and dichotomous tip branching occurred as in the wild-type. Few actin cables in Agwal1 cells were found to insert into the hyphal...

... pole body-independent microtubules. At the onset of bud formation two spherical tubulin structures focus microtubules towards the growth region, suggesting that they support polar growth in G 2 , while spindle pole body-nucleated astral microtubules participate in nuclear migration in M and early G 1...

...J. Wendland; P. Philippsen ABSTRACT In the filamentous ascomycete Ashbya gossypii , like in other filamentous fungi onset of growth in dormant spores occurs as an isotropic growth phase generating spherical germ cells. Thereafter, a switch to polarized growth results in the formation of the first...