...Robert Herzog; Koen van den Dries; Pasquale Cervero; Stefan Linder ABSTRACT Podosomes are actin-based adhesion and invasion structures in a variety of cell types, with podosome-forming cells displaying up to several hundreds of these structures. Podosome number, distribution and composition can...

...-polarized hMDMs. Using PTPε-specific siRNAs and expression of RPTPε and cyt-PTPε, we found that RPTPε is involved in monocyte adhesion and migration of M2-polarized hMDMs in response to LTD 4 . Altered organization of podosomes and higher phosphorylation of the inhibitory Y-722 residue of ROCK2 was also...

..., and act as scaffolds, bringing membrane and cellular components in close proximity in structures known as invadopodia or podosomes. Tks proteins, analogous to the related proteins p47 phox , p40 phox and NoxO1, also facilitate local generation of reactive oxygen species (ROS), which aid in signaling...

...Thomas Daubon; Pirjo Spuul; Florian Alonso; Isabelle Fremaux; Elisabeth Génot ABSTRACT Podosomes are dynamic cell–matrix contact structures that combine several key abilities, including adhesion, matrix degradation and mechanosensing. These actin-based cytoskeletal structures have been mostly...

...Linda Panzer; Leona Trübe; Matthias Klose; Ben Joosten; Johan Slotman; Alessandra Cambi; Stefan Linder ABSTRACT Podosomes are actin-rich adhesion structures that depend on Arp2/3-complex-based actin nucleation. We now report the identification of the formins FHOD1 and INF2 as novel components...

...Christian Gawden-Bone; Michele A. West; Vicky L. Morrison; Alexander J. Edgar; Sarah J. McMillan; Brian D. Dill; Matthias Trost; Alan Prescott; Susanna C. Fagerholm; Colin Watts ABSTRACT The dynamic properties of podosomes, their ability to degrade the underlying matrix and their modulation by Toll...

... that these RabGTPases are crucial in the regulation of MT1-MMP surface exposure, contact of MT1-MMP-positive vesicles with podosomes, extracellular matrix degradation in two and three dimensions, as well as three-dimensional proteolytic invasion of macrophages. Collectively, our results identify Rab5a, Rab8a and Rab14...

... that the protein is involved in the regulation of bone development. Two recent studies unveiled new potential functions of FGD1, in particular, its involvement in the regulation of the formation and function of invadopodia and podosomes, which are cellular structures devoted to degradation of the extracellular...

...Ridhirama Bhuwania; Susanne Cornfine; Zhiyou Fang; Marcus Krüger; Elizabeth J. Luna; Stefan Linder Podosomes are actin-rich adhesion and invasion structures. Especially in macrophages, podosomes exist in two subpopulations, large precursors at the cell periphery and smaller podosomes (successors...

...Lindsy R. Boateng; Christa L. Cortesio; Anna Huttenlocher Podosomes are dynamic actin-based structures that mediate adhesion to the extracellular matrix and localize matrix degradation to facilitate cell motility and invasion. Drebrin-like protein (DBNL), which is homologous to yeast mAbp1...

...Patricia Rottiers; Frédéric Saltel; Thomas Daubon; Benjamin Chaigne-Delalande; Viviane Tridon; Clotilde Billottet; Edith Reuzeau; Elisabeth Génot Podosomes are specialized plasma-membrane actin-based microdomains that combine adhesive and proteolytic activities to spatially restrict sites...

...Athanassios Dovas; Jean-Claude Gevrey; Alberto Grossi; Haein Park; Wassim Abou-Kheir; Dianne Cox Podosomes, adhesion structures capable of matrix degradation, have been linked with the ability of cells to perform chemotaxis and invade tissues. Wiskott-Aldrich Syndrome protein (WASp), an effector...

...-membrane actin filaments. Similar to focal adhesions and podosomes, invadopodia are cell-matrix adhesion sites. Indeed, invadopodia share several features with podosomes, but whether they are distinct structures is still a matter of debate. Invadopodia are built upon an N-WASP-dependent branched actin...

...Andrea Dorfleutner; YoungJin Cho; Deanne Vincent; Jess Cunnick; Hong Lin; Scott A. Weed; Christian Stehlik; Daniel C. Flynn AFAP-110 is an actin-binding and -crosslinking protein that is enriched in Src and phorbol ester (PE)-induced podosomes. In vascular smooth muscle cells endogenous AFAP-110...

... that p190B plays a crucial role in the regulation of MT1-MMP expression and cell-surface presentation, as well as subsequent MMP2 activation. p190B is involved in both local extracellular matrix degradation at podosomes and endothelial cell assembly into tube-like structures in Matrigel. In addition, whereas...

...Suzanne F. G. van Helden; Machteld M. Oud; Ben Joosten; Niels Peterse; Carl G. Figdor; Frank N. van Leeuwen Podosomes are dynamic adhesion structures found in dendritic cells (DCs) and other cells of the myeloid lineage. We previously showed that prostaglandin E2 (PGE 2 ), an important...

...) domains and a proline-rich (PR) region that is connected by a `linker' sequence to a coiled-coil (CC) domain. We show that CMS is a component of special actin-rich adhesion structures – podosomes – and demonstrate specific actin-binding properties of CMS. We have found that the entire C-terminal half...

...Chen Luxenburg; J. Thomas Parsons; Lia Addadi; Benjamin Geiger Osteoclasts are large, multinucleated cells that adhere to bone via podosomes, and degrade it. During osteoclast polarization, podosomes undergo reorganization from a scattered distribution, through the formation of clusters and ring...

...Olivier Collin; Philippe Tracqui; Angélique Stephanou; Yves Usson; Jocelyne Clément-Lacroix; Emmanuelle Planus In this study we analyse the formation and dynamics of specific actin-rich structures called podosomes. Podosomes are very dynamic punctual adhesion sites tightly linked to the actin...

...Florence Tatin; Christine Varon; Elisabeth Génot; Violaine Moreau The involvement of Src, Cdc42, RhoA and PKC in the regulation of podosome assembly has been identified in various cell models. In endothelial cells, the ectopic expression of constitutively active mutants of Src or Cdc42...