...Andrew P. Porter; Hannah Reed; Gavin R. M. White; Erinn-Lee Ogg; Helen J. Whalley; Angeliki Malliri ABSTRACT Centriole duplication is tightly controlled to maintain correct centriole number through the cell cycle. Key to this is the regulated degradation of PLK4, the master regulator of centriole...

...Yi Liu; Jaeyoun Kim; Reuben Philip; Vaishali Sridhar; Megha Chandrashekhar; Jason Moffat; Mark van Breugel; Laurence Pelletier ABSTRACT PLK4 has emerged as a prime target for cancer therapeutics, and its overexpression is frequently observed in various types of human cancer. Recent studies have...

... of procentriole emergence through focusing of polo-like kinase 4 (PLK4) and the resulting assembly of spindle assembly abnormal protein 6 (SAS-6) into a cartwheel element are evoked next. Subsequently, we cover the recruitment of peripheral components that include the pinhead structure, MTs and the MT-connecting...

... barrel-shaped structures, the centrioles, surrounded by the pericentriolar material (PCM), which nucleates microtubules. Centrosomes can form close to an existing structure (canonical duplication) or de novo . How centrosomes form de novo is not known. The master driver of centrosome biogenesis, PLK4...

... is therefore crucial for genome stability and embryogenesis. Centriole duplication requires the serine/threonine protein kinase Plk4. Here, we identify Cep78 as a human centrosomal protein and a new interaction partner of Plk4. Cep78 is mainly a centriolar protein that localizes to the centriolar wall...

..., the centriole duplication cycle is governed by Polo‐like kinase 4 (Plk4). Here, we identify the E3 ubiquitin ligase Mind bomb (Mib1) as a new interaction partner of Plk4. We show that Mib1 localizes to centriolar satellites but redistributes to centrioles in response to conditions that induce centriole...

...Qingwen Xu; Yuxia Zhang; Xunhao Xiong; Yan Huang; Jeffery L. Salisbury; Jinghua Hu; Kun Ling ABSTRACT Centriole biogenesis depends on the polo-like kinase (PLK4) and a small group of structural proteins. The spatiotemporal regulation of these proteins at pre-existing centrioles is essential...

...Katharina F. Sonnen; Anna-Maria Gabryjonczyk; Eduard Anselm; York-Dieter Stierhof; Erich A. Nigg Summary Polo-like kinase 4 (Plk4) is a key regulator of centriole duplication, but the mechanism underlying its recruitment to mammalian centrioles is not understood. In flies, Plk4 recruitment depends...

...Ramona Bahtz; Joerg Seidler; Marc Arnold; Uta Haselmann-Weiss; Claude Antony; Wolf D. Lehmann; Ingrid Hoffmann Centriole duplication occurs once per cell cycle and requires Plk4, a member of the Polo-like kinase family. A key component of the centrosome is the γ-tubulin ring complex (γ-TuRC...

..., and aberrations in centrosome number have been implicated in chromosomal instability and tumor formation. The centriolar protein Polo-like kinase 4 (Plk4) is a key regulator of centriole biogenesis and is crucial for maintaining constant centriole number, but the mechanisms regulating its activity and expression...

... from small dots to elongated fibers. Although γ-tubulin seems to exist as a ring complex, the abnormal structures do not support microtubule nucleation. Several lines of evidence suggest that the fibers correspond to a disorganized form of centriolar microtubules. Plk4, a mammalian homolog of ZYG-1...