..., which is dependent on the cytolinker plectin. Nocodazole treatment has a negligible effect on F-actin distribution but significantly alters the spatial arrangement of vimentin. We demonstrate that Cyto D treatment upregulates vimentin expression via reactive oxygen species-mediated activation of NF-κΒ...

... features. These hybrid cell-matrix adhesions, which are formed early during cell adhesion and spreading and at later stages of cell spreading, are present in the central region of the cells. They contain the CD151–α3β1/α6β4 integrin complexes and the cytoskeletal linker protein plectin...

... of the cytoskeleton to nuclear mechanotransduction remains unclear. In this study, we examine how crosstalk between the cytolinker plectin and F-actin controls keratin network organisation and the 3D nuclear morphology of keratinocytes. Using micro-patterned surfaces to precisely manipulate cell shape, we find...

...Selma Osmanagic-Myers; Stefanie Rus; Michael Wolfram; Daniela Brunner; Wolfgang H. Goldmann; Navid Bonakdar; Irmgard Fischer; Siegfried Reipert; Aurora Zuzuarregui; Gernot Walko; Gerhard Wiche ABSTRACT Mutations in the cytoskeletal linker protein plectin result in multisystemic diseases affecting...

...Jamal-Eddine Bouameur; Yann Schneider; Nadja Begré; Ryan P. Hobbs; Prakash Lingasamy; Lionel Fontao; Kathleen J. Green; Bertrand Favre; Luca Borradori Summary Plectin is a versatile cytolinker of the plakin family conferring cell resilience to mechanical stress in stratified epithelia and muscles...

...Ruben Postel; Mirjam Ketema; Ingrid Kuikman; José M. de Pereda; Arnoud Sonnenberg The outer nuclear membrane protein nesprin-3 binds the cytoskeletal linker protein plectin, which are proposed to anchor the intermediate filaments to the nuclear envelope. To investigate the function of nesprin-3...

...) tethered to costameric sarcolemma. Although there is emerging evidence that plectin links IFs to costameres through dystrophin-glycoprotein complexes (DGC), the molecular organization from plectin to costameres still remains unclear. Here, we show that plectin 1, a plectin isoform expressed in skeletal...

... with the cytoskeletal crosslinker protein plectin. Both nesprin-3α and plectin can form dimers, and dimerization of plectin is required for its interaction with nesprin-3α at the nuclear envelope, which is mediated by its N-terminal actin-binding domain. Additionally, overexpression of the plectin actin-binding domain...

...Reinhard Ackerl; Gernot Walko; Peter Fuchs; Irmgard Fischer; Matthias Schmuth; Gerhard Wiche Plectin, a widespread intermediate filament-based cytolinker protein capable of interacting with a variety of cytoskeletal structures and plasma membrane-bound junctional complexes, serves essential...

.... This protein shows a low degree of sequence similarity to trichohyalin, plectin and myosin heavy chain, and is a K8/18-binding protein. Among interactions between trichoplein and various IF proteins that we tested using two-hybrid methods, trichoplein interacted significantly with K16 and K18, and to some...

... correlation to alterations in the actin- and tubulin-based systems. Interestingly, the short-lived and rather small orthovanadate-induced cytokeratin granules contained the cytoskeletal crosslinker plectin but lacked the cytokeratin-solubilising 14-3-3 proteins. By contrast, the long-lived and larger...

...Lionel Fontao; Dirk Geerts; Ingrid Kuikman; Jan Koster; Duco Kramer; Arnoud Sonnenberg Plectin is a major component of the cytoskeleton and is expressed in a wide variety of cell types. It plays an important role in the integrity of the cytoskeleton by cross-linking the three filamentous networks...

... and characterized. The microtubule-binding properties of MACF2 C-terminal domain are similar to that of MACF. The GAR domain was originally found in Gas 2 protein and here we show that it can associate with MTs in transfected cells. Plectin and desmoplakin have GSR-containing domains at their C- termini and we...

...Ferdinand A. Steinböck; Branislav Nikolic; Pierre A. Coulombe; Elaine Fuchs; Peter Traub; Gerhard Wiche ABSTRACT Plectin, the largest and most versatile member of the cytolinker/plakin family of proteins characterized to date, has a tripartite structure comprising a central 200 nm-long α-helical...

...Takao Hijikata; Tohru Murakami; Michihiro Imamura; Noboru Fujimaki; Harunori Ishikawa ABSTRACT Plectin is a versatile linker protein which is associated with various types of cytoskeletal components and/or filaments including intermediate filaments, and its deficiency causes the disruption...