...Ankit Ghosh; Aastha Varshney; Sunil Kumar Narwal; Nirdosh; Roshni Gupta; Satish Mishra ABSTRACT Plasmodium sporozoites are the infective forms of the malaria parasite in the mosquito and vertebrate host. Gliding motility allows sporozoites to migrate and invade mosquito salivary glands...

... the Plasmodium liver stage. The protocol presented in this study allows expanding of pre-stained samples, which results in shorter incubation times, better preservation of some epitopes and the advantage that non-expanded controls can be performed alongside using the same staining protocol. The protocol...

... an extensive RSP complement, including two divergent RSP9 orthologues, necessary for flagellar beating and swimming. Detailed structural analysis showed that neither orthologue is needed for axoneme assembly in Leishmania . In contrast, Plasmodium has a reduced set of RSPs including a single RSP9 orthologue...

...Jessica Kehrer; Emma Pietsch; Julia Heinze; Tobias Spielmann; Friedrich Frischknecht ABSTRACT Malaria is a devastating mosquito-borne parasitic disease that manifests when Plasmodium parasites replicate within red blood cells. During the development within the red blood cell, the parasite digests...

... its complex life cycle, each time disrupting, in a highly regulated manner, the membranes or cyst wall that entrap the parasites. This Cell Science at a Glance article and accompanying poster summarises our current knowledge of the morphological features of egress across the Plasmodium life cycle...

... requires chromosome replication and precise segregation to ensure daughter cells have identical genomic copies. Species of the genus Plasmodium , the causative agents of malaria, display remarkable aspects of nuclear division throughout their life cycle to meet some peculiar and unique challenges to DNA...

...Catherine A. Moreau; Katharina A. Quadt; Henni Piirainen; Hirdesh Kumar; Saligram P. Bhargav; Léanne Strauss; Niraj H. Tolia; Rebecca C. Wade; Joachim P. Spatz; Inari Kursula; Friedrich Frischknecht ABSTRACT During transmission of malaria-causing parasites from mosquito to mammal, Plasmodium...

...Xiaoying Liang; Kevin J. Hart; Gang Dong; Faiza A. Siddiqui; Aswathy Sebastian; Xiaolian Li; Istvan Albert; Jun Miao; Scott E. Lindner; Liwang Cui ABSTRACT In this study, we characterized the Puf family gene member Puf3 in the malaria parasites Plasmodium falciparum and Plasmodium yoelii...

...Stanley C. Xie; Con Dogovski; Eric Hanssen; Francis Chiu; Tuo Yang; Maria P. Crespo; Che Stafford; Steven Batinovic; Silvia Teguh; Susan Charman; Nectarios Klonis; Leann Tilley ABSTRACT Current first-line artemisinin antimalarials are threatened by the emergence of resistant Plasmodium falciparum...

... illustrated guide to the ultrastructure of Plasmodium falciparum asexual blood stages.   Parasitol. Today   16 , 427 – 433 . 10.1016/S0169-4758(00)01755-5 Beck   J. R. , Rodriguez-Fernandez   I. A. , de Leon   J. C. , Huynh   M-H. , Carruthers   V. B. , Morrissette   N. S...

... of PfPuf2, a member of the Puf family of translational repressors, in gametocytogenesis of Plasmodium falciparum . Tagging of the endogenous PfPuf2 protein with green fluorescent protein showed that PfPuf2 was expressed in both male and female gametocytes, and the protein was localized in the cytoplasm...

...Nicole S. Struck; Susann Herrmann; Christine Langer; Andreas Krueger; Bernardo J. Foth; Klemens Engelberg; Ana L. Cabrera; Silvia Haase; Moritz Treeck; Matthias Marti; Alan F. Cowman; Tobias Spielmann; Tim W. Gilberger Plasmodium falciparum , the causative agent of malaria, relies on a complex...

...Michelle D. Lazarus; Timothy G. Schneider; Theodore F. Taraschi The current model for hemoglobin ingestion and transport by intraerythrocytic Plasmodium falciparum malaria parasites shares similarities with endocytosis. However, the model is largely hypothetical, and the mechanisms responsible...

... that only binds to the tetraspanin CD81 associated with other tetraspanins, we show that membrane cholesterol contributes to the organization of tetraspanin microdomains on the surface of live cells. Furthermore, our data demonstrate involvement of host membrane cholesterol during infection by Plasmodium...

...Nicole S. Struck; Suzana de Souza Dias; Christine Langer; Matthias Marti; J. Andrew Pearce; Alan F. Cowman; Tim W. Gilberger Plasmodium falciparum, the causative agent of malaria, relies on a sophisticated protein secretion system for host cell invasion and transformation. Although the parasite...

..., and thus resemble multivesicular bodies. To determine whether multivesicular body endosomal intermediates are formed in Apicomplexa, we used the Plasmodium falciparum homolog of the class E gene, Vps4 , as a probe. Endogenous P. falciparum Vps4 (PfVps4) localized to the cytoplasm of P. falciparum...

...-hybrid screen we identified MTIP, a novel 24 kDa protein that interacts with MyoA. Deletion analysis shows that the 15 amino-acid C-terminal tail domain of MyoA, rather than the neck domain, specifically interacts with MTIP. In Plasmodium sporozoites MTIP localizes to the inner membrane complex (IMC...

...Akinola Adisa; Frank R. Albano; John Reeder; Michael Foley; Leann Tilley The malaria parasite, Plasmodium falciparum , spends part of its life cycle inside the enucleated erythrocytes of its human host. The parasite modifies the cytoplasm and plasma membrane of its host cell by exporting proteins...

...Klaus Lingelbach; Keith A. Joiner ABSTRACT Plasmodium and Toxoplasma belong to a group of unicellular parasites which actively penetrate their respective mammalian host cells. During the process of invasion, they initiate the formation of a membrane, the so-called parasitophorous vacuolar membrane...

...Kenji Murakami; Kazuyuki Tanabe; Suehisa Takada ABSTRACT A cation-transporting ATPase gene of Plasmodium yoelii was cloned from the parasite genomic library using an oligonucleotide probe derived from a conserved amino acid sequence of the phosphorylation domain of the aspartyl phosphate family...