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Keywords: Photobleaching
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Journal Articles
J Cell Sci (2020) 133 (10): jcs240796.
Published: 27 May 2020
... and shortens imaging time by approximately an order of magnitude as compared to standard volumetric confocal imaging. With faster imaging, radiation exposure to the sample is reduced, resulting in less fluorophore photobleaching and potential photodamage. The implementation minimally requires two synchronized...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (2): 288–294.
Published: 15 January 2014
...Liang Gao; Alejandro Garcia-Uribe; Yan Liu; Chiye Li; Lihong V. Wang ABSTRACT We present a generic sub-diffraction-limited imaging method – photobleaching imprinting microscopy (PIM) – for biological fluorescence imaging. A lateral resolution of 110 nm was measured, more than a twofold improvement...
Journal Articles
J Cell Sci (2008) 121 (7): 1128–1137.
Published: 01 April 2008
... is a crucial activity. Here, we apply photobleaching strategies within the nucleus using domain-deleted MECP2 proteins as well as naturally occurring point mutations identified in individuals with the neurodevelopmental disorder Rett syndrome (RTT). These studies reveal that MECP2 is transiently associated...
Includes: Supplementary data
Journal Articles
J Cell Sci (2007) 120 (14): 2318–2327.
Published: 15 July 2007
... for membrane flow from the leading edge, as photobleached or photoactivated marks in the plasma membrane move forward roughly in step with the leading edge, rather than backwards from it. Third, we find that the surface area of a cell - measured from confocal reconstructions - constantly fluctuates during...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2006) 119 (19): 4101–4116.
Published: 01 October 2006
... high throughput microscopy approach. In support of the nuclear receptor hit-and-run model, photobleaching studies provided direct evidence of very transient ER-array interactions, and revealed ligand-dependent changes in k off . ERα-truncation mutants indicated that helix-12 and interactions with co...
Includes: Supplementary data
Journal Articles
J Cell Sci (2006) 119 (6): 1092–1104.
Published: 15 March 2006
... ) 5 12 2005 © The Company of Biologists Limited 2006 2006 Nucleocytoplasmic shuttling STAT Proteins Photobleaching CRM1-independent export Type-I-interferon-signaling Interferons (IFNs) reprogram gene expression through the activation of signal transducer and activator...
Includes: Supplementary data
Journal Articles
J Cell Sci (2004) 117 (25): 6129–6141.
Published: 01 December 2004
... embryo neurons and performed time-lapse imaging of tau-labeled MTs. Tau uniformly labeled individual MTs regardless of their assembly/disassembly status and location along the axon. Photobleaching experiments indicated that interaction of tau with MTs is very dynamic, with a half-time of fluorescence...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2004) 117 (18): 4113–4125.
Published: 15 August 2004
... in living cells using fusions of Smad2 and Smad4 with enhanced GFP. We first establish that the GFPSmad fusions behave like wild-type Smads in a variety of cellular assays. We go on to demonstrate directly, using photobleaching experiments, that Smad2 and Smad4 shuttle between the cytoplasm and nucleus...
Journal Articles
J Cell Sci (2003) 116 (9): 1707–1717.
Published: 01 May 2003
... laser (20 mW) was used to perform all live cell and photobleaching experiments. The images were acquired by using a wavelength interval between 500-580 nm. In the time-lapse experiment, cells were followed for 3 hours after proteasome inhibitor addition. Images were acquired every 120 seconds...
Journal Articles
J Cell Sci (2001) 114 (21): 3885–3897.
Published: 01 November 2001
... to substantially reduce the level of ATP in living cells and we used Taxol to halt microtubule dynamics. Both treatments slowed the dynamics of 5×GFP-EMTB speckles observed by FSM. We also used fluorescence recovery after photobleaching (FRAP) to quantify the half-time of binding and dissociation of the 5×GFP-EMTB...