... Summary: To study the intricate metabolic network of sphingolipids, minimally modified probes are the tools of choice. We review progress made by using these probes together with the power of click chemistry. Expansion microscopy Lipid metabolism Lipid transport Photoactivation...

...-1/ Summary: The small G-protein Ran is an important non-centrosomal microtubule regulator in neurons, and the active GTP-bound Ran is transported by actin waves to its target region. Microtubules Growth cone-like waves Photoactivation Neuronal development +TIPs Ministry...

...Maryna Kapustina; Tracy-Ann Read; Eric A. Vitriol ABSTRACT Photoactivation allows one to pulse-label molecules and obtain quantitative data about their behavior. We have devised a new modeling-based analysis for photoactivatable actin experiments that simultaneously measures properties of monomeric...

... recovery after photobleaching (FRAP) and mEos2 photoactivation to monitor the dynamics of IMC biogenesis and turnover during the replication of Toxoplasma gondii tachyzoites. These studies reveal that the formation of the T. gondii IMC involves two distinct steps – de novo assembly during daughter IMC...

... Optical highlighters Photoactivation Photoconversion Photoswitching Live-cell imaging The rapid rise of fluorescent proteins (FPs) as a fundamental staple of biomedical research has often been termed a revolution, the first phase of which began just over a decade ago when the original...

... for membrane flow from the leading edge, as photobleached or photoactivated marks in the plasma membrane move forward roughly in step with the leading edge, rather than backwards from it. Third, we find that the surface area of a cell - measured from confocal reconstructions - constantly fluctuates during...