... recruits the adaptor protein Nck to form membrane clusters, which are important for nephrin function. Tyrosine mutations result in abnormal cluster morphology and disrupted endocytosis. Podocyte Nephrin Phosphotyrosine Nck Dynamin Clustering Canadian Institutes of Health Research...

... cells was counted after fixation. Fixed cells were blocked with 1% BSA and stained for 1 hour at 37°C with rhodamine phalloidin for filamentous actin (Molecular Probes), with a monoclonal antibody pTyr-clone 4G10 for phosphotyrosine (1:100; Upstate Biotechnology, Lake Placid, NY...

... those previously described in females. Mature ring canal walls in males lack actin and appear to derive directly from structural proteins associated with the contractile ring. Ring canal assembly in males, as in females, initiates during cytokinesis with the appearance of a ring of phosphotyrosine...

... in this manner. In each case the major proteins detected using anti-phosphotyrosine antibodies have molecular masses of approximately 170, 150, 125, 70 and 50 kDa. As a first step in determining the function of this protein modification in embryogenesis we have initiated a study to identify these phosphoproteins...

...Rajesh K. Naz; Khaliq Ahmad; Rakesh Kumar ABSTRACT The monoclonal anti-phosphotyrosine antibody (PTA) recognized proteins related to relative molecular mass regions of 94 000±3000 and 46 000±3000 M r on Western blots of detergent-solubilized non-capacitated human sperm extract (HSE). The pattern...