..., and that phosphatidylserine is involved in the activation of both signaling pathways. We also demonstrated that the Rho family GTPase Cdc42 contributes to the plasma membrane stress-induced activation of TORC2–Ypk1/2 signaling. Furthermore, we revealed that phosphatidylinositol-specific phospholipase C, Plc1, contributes...

... fixed intracellular sites. Ca 2+ puff Endoplasmic reticulum IP 3 IP 3 receptor Muscarinic receptor Phospholipase C Most Ca 2+ signals in electrically inexcitable cells are initiated by receptors that stimulate phospholipase C (PLC) and, thereby, formation of inositol 1,4,5...

... C was identified as a pivotal player during cell death, because modulation of the phospholipase C signaling pathway and deletion of PLC-2, which we show to be involved in hyphal development, results in an inability to trigger the characteristic staurosporine-induced Ca 2+ signature. Using Δ cch-1...

... show that phospholipase C (PLC) preferentially cleaves oxidised lipids. Because of this, an increase in the rate of lipid peroxidation leads to increased Ca 2+ release from endoplasmic reticulum (ER) stores in response to physiological activation of purinoreceptors with ATP. Both vitamin E and its...

.... A. , Wickner   W. ( 2004 ). Diacylglycerol and its formation by Phospholipase C regulate Rab- and SNARE-dependent yeast vacuole fusion . J. Biol. Chem.   279 , 53186 - 53195 . Jun   Y. , Thorngren   N. , Starai   V. J. , Fratti   R. A. , Collins   K. , Wickner   W. ( 2006...

...@USherbrooke.ca ) WDR36 Thromboxane A 2 GPCR G protein Phospholipase C G-protein-coupled receptors (GPCRs) are the largest family of membrane receptors responsible for mediating extracellular to intracellular signalling. They respond to a variety of stimuli, including light, hormones...

...Michail Nomikos; Khalil Elgmati; Maria Theodoridou; Brian L. Calver; George Nounesis; Karl Swann; F. Anthony Lai Phospholipase C-zeta (PLCζ) is a strong candidate for the mammalian sperm-derived factor that triggers the Ca 2+ oscillations required for egg activation at fertilization. PLCζ lacks...

..., inhibition of myosin II induced the outgrowth of F-actin-rich surface protrusions. The development of these protrusions was abrogated after inhibition of F-actin polymerization or of phospholipase C (PLC) activity, as well as after overexpression of a dominant-negative ADF/cofilin. Surface protrusions were...

...+ signaling Caveolin-1 Phospholipase C Caveolae are prominent protein domains in membranes that are believed to be involved in receptor desensitization, endocytosis, transcytosis, vesicle trafficking, cell migration and other cellular events (for reviews, see Anderson, 1998 ; Marx, 2001 ; Navarro...

... technique, we report that plasma membrane PtdIns(4,5) P 2 becomes transiently depleted upon activation of exocytosis, and is not detected on the membranes of fusing granules. Depletion is caused by phospholipase C activity, and is mandatory for exocytosis. Although phospholipase C is required for Ca 2...

... for correspondence (e-mail: bradford_berk@urmc.rochester.edu ) 6 2 2006 © The Company of Biologists Limited 2006 2006 GIT1 G-protein-coupled receptor Endosomes Phospholipase C Among the largest gene families in the mammalian genome are the G-protein-coupled receptors (GPCRs...

...Sophia Thore; Oleg Dyachok; Erik Gylfe; Anders Tengholm Phospholipase C (PLC) regulates various cellular processes by catalyzing the formation of inositol-1,4,5-trisphosphate (IP 3 ) and diacylglycerol from phosphatidylinositol-4,5-bisphosphate (PIP 2 ). Here, we have investigated the influence...

... seems counterintuitive, as inhibition of phospholipase C would be expected to increase cellular levels of PtdIns(4,5) P 2 and hence increase actin polymerization. Our confocal images suggest, however, that F-actin might accumulate at the poles of U7-treated cells, consistent with the idea that PtdIns...

...-dependency is unknown, despite their importance for egg activation and embryo development. We recently identified a novel, sperm-specific phospholipase C (PLCzeta; PLCζ) that triggers Ca 2+ oscillations similar to those caused by sperm. We show that PLCζ-induced Ca 2+ oscillations also occur exclusively...

... treatments do not affect growth, we suggest that the small conductance channel generates the obligatory tip-high Ca 2+ gradient during hyphal growth. Since IP 3 production must be catalyzed by tip-localized phospholipase C, we show that a number of phospholipase C inhibitors [neomycin,1-[6-((17β-3...

...Ansuman Chattopadhyay; Graham Carpenter Phospholipase C-γ1, a tyrosine kinase substrate, hydrolyses phosphatidylinositol 4,5-bisphosphate to produce inositol 1,4,5-trisphosphate and diacylglycerol, which act as second messenger moleculesto mobilize intracellular calcium and activate protein kinase...

... phosphorylation of PtdIns(4,5) P 2 by phosphoinositide 3-kinases, was recruited to the plasma membrane but not to EGF- or transferrin-containing endosomes in response to EGF stimulation. These results suggest that while many internalized receptors continue to engage intracellular enzymes, the phospholipase C...

.... Consistent with previous observations, phospholipase C (PLC) inhibitors block anesthetic effects on the PIP 2 and cytoplasmic Ca 2+ levels, as well as the drop in adhesion. Therefore, we suggest that PLC activity is increased by amine anesthetics at the cytoplasmic surface of the plasma membrane, which...

... 2+ is needed mainly at a site located on the outer side of the cell membrane, where it activates uvomorulin and triggers the participation of the cellular components mentioned above, via G-proteins associated with phospholipase C and protein kinase C. In principle, the sites of all these molecules...