... of the chemoattractant cyclic adenosine monophosphate (cAMP) activates phosphoinositide 3-kinases (PI3Ks), which increase levels of phosphatidylinositol 3,4,5-trisphosphate [PI(3,4,5)P 3 ] and active Ras at the D. discoideum cell membrane to form polymerized actin-rich pseudopods projecting towards the source of cAMP...

... functions. Phosphoinositide PtdIns Signaling PIPK Plasma membrane PI3K PLC National Institutes of Health http://dx.doi.org/10.13039/100000002 2R35GM119412 5F31CA247349-02 1R03TR003624-01 National Science Foundation http://dx.doi.org/10.13039/100000001 MCB-2048060...

... of phosphoinositide kinases and the Dishevelled protein Dvl3 in the context of two receptor signaling cascades. Simultaneous overexpression of PI4KIIIα (also known as PI4KA) and PIP5KIγ (also known as PIP5K1C) had a synergistic effect on PtdIns P 2 synthesis that was recapitulated by overexpression of Dvl3...

... the recruitment of 5-inositol phosphatases to circular ruffles. Macropinocytosis GTPase Rab Phosphatase Phosphoinositide EGF Ruffling Canadian Institutes of Health Research http://dx.doi.org/10.13039/501100000024 FDN-143202 Heart and Stroke Foundation of Canada http...

... of autophagosome biogenesis. Autophagy Endosome Phosphoinositide Recycling Kreftforeningen http://dx.doi.org/10.13039/100008730 182698 Helse Sør-Øst RHF http://dx.doi.org/10.13039/501100006095 2018081 European Research Council http://dx.doi.org/10.13039/100010663...

... by the PIKfyve complex regulates membrane remodeling of early melanosomes; this controls the import–export balance that is required for functional amyloid formation and melanosome identity. PIKfyve FIG4 VAC14 PI(3,5)P2 Phosphoinositide Melanosome Lysosome PMEL Amyloid The protein complex...

...) is a phosphoinositide 5-phosphatase for phosphatidylinositol (3,4,5)-trisphosphate [PI(3,4,5)P3] and phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2]. Here we show, through depletion of SHIP2 in triple negative MDA-MB-231 cells and the use of SHIP2 inhibitors, that cell migration appears to be positively controlled...

...-phosphatase that dephosphorylates PI(4,5)P 2 , cause Lowe syndrome. Previously we showed that OCRL localizes to the primary cilium, which has a distinct membrane phospholipid composition, but disruption of phosphoinositides in the ciliary membrane is poorly understood. Here, we demonstrate that cilia from...

...William's Elong Edimo; Somadri Ghosh; Rita Derua; Veerle Janssens; Etienne Waelkens; Jean-Marie Vanderwinden; Pierre Robe; Christophe Erneux ABSTRACT Phosphoinositides, particularly phosphatidylinositol (3,4,5)-trisphosphate [PI(3,4,5)P3] and phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2...

... of a phosphoinositide-regulated stress sensor. * Author for correspondence ( [email protected] ) Competing interests The authors declare no competing or financial interests. 22 4 2015 18 8 2015 © 2015. Published by The Company of Biologists Ltd 2015 Summary: Yeast...

... to growth factor stimulation and local production of the phosphoinositide lipid phosphatidylinositol (3,4,5)-trisphosphate [PtdIns(3,4,5) P 3 ] following phosphoinositide 3-kinase (PI3K) activation. The metabolism and turnover of phosphoinositides is complex – they act as signalling molecules as well...

..., distribution and reproduction in any medium provided that the original work is properly attributed. Summary: RDGB regulates PtdIns and PtdIns(4,5) P 2 homeostasis during G-protein-activated phospholipase C signalling in Drosophila . RDGB Lipid transfer Phosphoinositide PITP...

..., the adaptor protein complex 2 (AP2) has been secondarily lost, suggesting mechanistic divergence. Here, we characterise two phosphoinositide-binding monomeric clathrin adaptors, T. brucei (Tb)EpsinR and TbCALM, which in trypanosomes are represented by single genes, unlike the expansions present in animals...

... to be clarified. Here, we examined the phosphoinositide-lipid-binding capabilities of full-length SNX27, and discovered a new PtdIns P -binding site within the C-terminal 4.1, ezrin, radixin, moesin (FERM) domain. This binding site showed a clear preference for bi- and tri-phosphorylated phophoinositides...

... of cytosolic Ca 2+ response to high concentration of AngII in HEK293 cells that express wild type but not truncated AGTR1 (AT1a receptor), missing the phosphorylation sites. Efr3 depletion had minimal effect on the recovery of plasma membrane phosphoinositides during stimulation, and AT1 receptors still...

...Steve Jean; Amy A. Kiger ABSTRACT The phosphoinositide 3-kinase (PI3K) family is important to nearly all aspects of cell and tissue biology and central to human cancer, diabetes and aging. PI3Ks are spatially regulated and multifunctional, and together, act at nearly all membranes in the cell...

...-positive early endosome. To elucidate the molecular mechanism for the phosphoinositide-independent association of SNX15 with clathrin-decorated elements of the early endocytic network we performed proteomics on SNX15 immuno-isolates. GFP-nanotrap pull-downs of cell extracts derived from HeLa cells...

.... * These authors contributed equally to this work ‡ Author for correspondence ( [email protected] ) 7 11 2011 © 2012. 2012 Fusion Phosphoinositide SNARE HOPS Phospholipase C Actin dynamics Membrane trafficking is utilized by all eukaryotes for the transportation of cargo...

.... * Present address: Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA ‡ Author for correspondence ( [email protected] ) 13 4 2011 © 2011. 2011 Store-operated Ca 2+ entry Lipid raft Phosphoinositide STIM1 Orai1...

...; Mikko J. Frilander; Harri Savilahti; Hannu Sariola; Kirsi Sainio; Sanna Lehtonen; Jussi Taipale; Marjo Salminen; Pekka Lappalainen MIM/MTSS1 is a tissue-specific regulator of plasma membrane dynamics, whose altered expression levels have been linked to cancer metastasis. MIM deforms phosphoinositide...