... the regulation of mTORC2 activity, but proposed mechanisms include a role for phosphatidylinositol (3,4,5)-trisphosphate – which is produced by class I phosphatidylinositol 3-kinases (PI3Ks), well-characterized Ras effectors. Therefore, the relationship between Ras, PI3K and mTORC2, in both normal physiology...

... in the platelet-derived growth factor (PDGF)-induced activation of phosphatidylinositol 3-kinase (PI3K)-Akt signaling for cell survival. This complex was associated with PDGF receptor on the plasma membrane at cell-cell adhesion sites. Thus, the nectin-afadin complex is involved in PDGF-induced cell survival...

...Erin E. Johnson; Jean H. Overmeyer; William T. Gunning; William A. Maltese The human type III phosphatidylinositol 3-kinase, hVps34, converts phosphatidylinositol (PtdIns) to phosphatidylinositol 3-phosphate [PtdIns(3) P ]. Studies using inhibitors of phosphatidylinositide 3-kinases have indicated...

... of Biologists Limited 2005 2005 Multilamellar bodies Cholesterol Lysosomes Phosphatidylinositol 3-kinase Autophagy Autophagic vacuoles Multilamellar bodies are lysosomal organelles containing multiple concentric membrane layers. MLBs vary in size from 100 to 2400 nm and are found in various...

... in vitro compared with Pafr +/+ zygotes ( P <0.02), those that developed to blastocysts had fewer cells ( P <0.001) and more cells with fragmented nuclei ( P <0.001). The inhibition of 1- O -phosphatidylinositol 3-kinase (LY294002 (3 μM and 15 μM) and wortmannin (10 nM and 50 nM)) caused a dose...

...Michaela Luconi; Vinicio Carloni; Fabio Marra; Pietro Ferruzzi; Gianni Forti; Elisabetta Baldi Sperm motility is regulated by a complex balance between kinases and phosphatases. Among them, phosphatidylinositol 3-kinase (PI 3-kinase) has been recently suggested to negatively regulate sperm motility...

... was insensitive to treatment with pertussis toxin, in contrast to chemotactic-peptide-induced shape changes, which were completely abolished by this treatment. Thus,colchicine does not appear to act via activating heterotrimeric G i proteins. Colchicine does also not seem to act via phosphatidylinositol 3-kinase...

... of phosphatidylinositol 3-kinase was diffusely punctate, and GLUT4myc was perinuclear. After 3 minutes of insulin treatment, actin reorganized to form structures; these structures protruded from the dorsal surface of the myotubes by 10 minutes and condensed in the myoplasm into less prominent foci at 30 minutes. The p85...

... signals are transduced from the plasma membrane to the nucleus are less known. Recent evidence indicates that phosphatidylinositol 3-kinase (PI3-kinase) could be activated by a direct association with the activated IL-1 receptor. In this study we analyzed the effects of IL-1 on the intracellular...

..., and the CNRS. C. De Nadai acknowledges receipt of an ARC fellowship. REFERENCES Arcaro , A. and Wymann , M. ( 1993 ). Wortmannin is a portent phosphatidylinositol 3-kinase inhibitor: the role of phosphatidylinositol 3,4,5-trisphosphate in neutrophil responses . Biochem. J . 296 , 297...