...Eirini Tsekitsidou; Cassandra J. Wong; Idil Ulengin-Talkish; Angela I. M. Barth; Tim Stearns; Anne-Claude Gingras; Jennifer T. Wang; Martha S. Cyert ABSTRACT Calcineurin, or protein phosphatase 2B (PP2B), the Ca 2+ and calmodulin-activated phosphatase and target of immunosuppressants, has many...

...Thomas Kokot; Maja Köhn ABSTRACT Protein phosphorylation on serine and threonine residues is a widely distributed post-translational modification on proteins that acts to regulate their function. Phosphoprotein phosphatases (PPPs) contribute significantly to a plethora of cellular functions through...

... formation. Importantly, we found that the removal of PtdIns(4,5)P 2 is dependent on Rab5, through the Rab5-mediated recruitment of the inositol 5-phosphatases OCRL and Inpp5b, via APPL1. Knockdown of OCRL and Inpp5b, or APPL1, prevented macropinosome closure without affecting ruffling. We therefore propose...

..., under the master control of Cdk1. During entry into mitosis, Cdk1, in collaboration with MASTL kinase, represses the activity of the major mitotic protein phosphatases, PP1 and PP2A, thereby ensuring mitotic substrates remain phosphorylated. For cells to complete and exit mitosis, these phosphorylation...

... declares no competing or financial interests. © 2015. Published by The Company of Biologists Ltd 2015 Cell division Cytokinesis Kinase Phosphatase Signalling Yeast Box 1. Counterparts of the SIN in other eukaryotes The signalling architecture of the SIN resembles the budding yeast...

..., tetrabromobenzotriazole and calyculin, which are active against CDK5, MKK1 (also known as MAP2K1), GSK3β, CK1, CK2 and protein phosphatase 1 (PP1), respectively. Sequential phosphorylation by CDK5 and GSK3β mediated the neurofilament–neurofilament associations. The MAPK pathway (i.e. MKK1 to ERK1/2) was found...

.... Published by The Company of Biologists Ltd 2014 Aquaporin AQP2 Phosphorylation Phosphatase Ubiquitylation Vasopressin Analysis of the ubiquitylation and phosphorylation datasets at PhosphoSite demonstrated that there are 95 other reported peptides (rat, mouse, human) that contain...

... MAPK Sty1 (also known as Spc1 or Phh1) is highly activated in response to a variety of external stresses. To avoid segregation of damaged organelles or chromosomes, strong Sty1 activation transiently blocks mitosis and cell division until such stresses have been dealt with. MAPK phosphatases...

...-transformed fibroblasts, osteoclasts and certain highly invasive cancer cells. Several protein tyrosine kinases have been shown to be important for the formation of podosome rosettes, but little is known regarding the role of protein tyrosine phosphatases in this process. We found that knockdown of the Src...

... transitions are abrupt rather than gradual. These considerations lead to a general quantitative model of cell cycle control, in which opposing kinase and phosphatase activities have an essential role in ensuring dynamic transitions. Cdk1 is embedded in nested futile cycles, as it is a substrate for one...

..., the importance of protein phosphatases is often overlooked. Recent studies, however, have highlighted the considerable role of protein phosphatases in both the spatial and temporal control of protein kinase activity, and the modulation of substrate phosphorylation. Here, we will focus on recent advances in our...

.... Phosphatidylinositol-3-phosphate (PtdIns3 P ) is concentrated on autophagic vesicles and recruits effector proteins that are crucial for this process. The production of PtdIns3 P by the class III phosphatidylinositol 3-kinase Vps34, has been well established; however, protein phosphatases that antagonize this early...

... required for both early and late localization of TAN at the CDS. Finally, we show that phosphatase activity is required for maintenance of early but not late TAN localization at the CDS. We propose a new model in which TAN is actively recruited to the CDS by several mechanisms, indicating that the CDS...

... in the nucleus have been characterized, little is known about the dephosphorylation of Smads in the cytoplasm. In a proteomic screen for proteins interacting with the BMP type-II receptor, we found the regulatory Bβ subunit of PP2A. PP2A is one of the major serine/threonine phosphatases involved in cell-cycle...

...Tanja Tamguney; David Stokoe The functions ascribed to PTEN have become more diverse since its discovery as a putative phosphatase mutated in many human tumors. Although it can dephosphorylate lipids and proteins, it also has functions independent of phosphatase activity in normal and pathological...

... was observed in U87MG cells, which lack the PtdIns(3,4,5) P 3 phosphatase, PTEN. Re-expression of PTEN into U87MG cells ablated plasma membrane PtdIns(3,4,5) P 3 , but not the nuclear pool of this lipid even when PTEN was targeted to nuclei. These data have important implications for the versatility of PI 3...

... continuous MEK (also known as MAPK kinase) activity upstream of ERK and is rapidly reduced by the operation of phosphatases. We measured quantitatively the speed of ERK2 shuttling between nucleus and cytoplasm and determined that shuttling accelerated after ERK activation, becoming fast enough not to be rate...

...Estelle Schmitt; Rose Boutros; Carine Froment; Bernard Monsarrat; Bernard Ducommun; Christine Dozier CDC25B is one of the three human phosphatases that activate the CDK-cyclin complexes, thereby triggering cell-cycle progression and division. Commitment to early mitotic events depends...

...Óscar Lorenzo; Sylvie Urbé; Michael J. Clague The myotubularins are a large family of lipid phosphatases with specificity towards PtdIns3 P and PtdIns(3,5) P 2 . Each of the 14 family members bears a signature phosphatase domain, which is inactive in six cases due to amino acid changes...

...Jennifer S. Jamieson; David A. Tumbarello; Maxime Hallé; Michael C. Brown; Michel L. Tremblay; Christopher E. Turner The tyrosine phosphatase PTP-PEST has been implicated in the regulation of cell spreading and migration through dephosphorylation of focal adhesion proteins and inhibition of Rac...