.... Strikingly, both absence and overexpression of vacuolins induced a strong downregulation of myosin VII (also known as MyoI) expression, as well as its binding partner talin A. Episomal expression of myosin VII fully rescued defects in uptake and adhesion but not in phagosome maturation. These results suggest...

... find that internalized particles are mechanically deformed in macrophages, suggesting that the process of phagocytosis also employs a ‘chewing’ step to help destroy unwanted targets in macrophages. Macrophages Phagocytosis Complement Phagosome maturation Natural Sciences...

... Gopaldass , N. , Patel , D. , Kratzke , R. , Dieckmann , R. , Hausherr , S. , Hagedorn , M. , Monroy , R. , Krüger , J. , Neuhaus , E. M. , Hoffmann , E. , et al. ( 2012 ). Dynamin A, Myosin IB and Abp1 couple phagosome maturation to F-actin binding . Traffic   13...

... dendritic cells, the membrane component of the NADPH oxidase NOX2 complex is initially recruited to phagosomes from the plasma membrane, and oxidized NOX2 complex subunits are replenished from a lysosomal pool. NOX2 SNARE Phagocytosis Dendritic cell Reactive oxygen species Phagosome maturation...

... yeasts to humans, mediates the digestion of these apoptotic corpses. CCZ-1 seems to act in lysosome biogenesis and phagosome maturation by recruiting the GTPase RAB-7 over the phagosome. In a screening for maternal-effect lethal mutations on chromosome V of C. elegans , we found that three alleles...

... phagosomes can transiently induce assembly of actin coat, which forms a barrier preventing phagosome-lysosome docking and fusion. The capacity of phagosomes to assemble actin is enhanced in cells exposed to increased phagocytic load, which also exhibit a delay in phagosome maturation. Parallel analysis...

...-loaded lysosomes. Inhibition of phagolysosome fusion could be attributed, at least in part, to the mycobacterial cell wall glycolipid lipoarabinomannan, and further analysis showed complete rescue of phagosome maturation when cells were pretreated with vitamin D3 before exposure to lipoarabinomannan...

... of Rab7 in phagosomal maturation. Expression of GFP-Rab7 resulted in labeling of both early and late phagosomes containing yeast, but not forming phagocytic cups. In order to determine if Rab7 played a role in regulating membrane traffic between the endo/lysosomal system and maturing phagosomes, latex...