...Markus Rudowitz; Ralf Erdmann ABSTRACT Peroxisomes are involved in a multitude of metabolic and catabolic pathways, as well as the innate immune system. Their dysfunction is linked to severe peroxisome-specific diseases, as well as cancer and neurodegenerative diseases. To ensure the ability...

...Tina A. Schrader; Ruth E. Carmichael; Markus Islinger; Joseph L. Costello; Christian Hacker; Nina A. Bonekamp; Jochen H. Weishaupt; Peter M. Andersen; Michael Schrader ABSTRACT Peroxisome membrane dynamics and division are essential to adapt the peroxisomal compartment to cellular needs...

... to the cytosol and to peroxisomes and is targeted to peroxisomes via association with Mdh3 and a Pex5-dependent piggybacking mechanism. This dual localization of Mdh2 contributes to our understanding of the glyoxylate cycle and provides a new perspective on compartmentalization of cellular metabolism, which...

.... lysosomes, mitochondria and peroxisomes) by taking advantage of the extraordinarily high affinity between the twin strep tag and streptavidin variants. With this method, we can isolate desired organelles with high purity and yield in 3 min from the post-nuclear supernatant of mammalian cells or less than 8...

... of correctly targeting a TA protein to its appropriate membrane, the mechanisms and signals involved are not fully understood. In this study, we identify additional peroxisomal TA proteins, discover more proteins that are present on multiple organelles, and reveal that a combination of TMD hydrophobicity...

...Ido Yofe; Kareem Soliman; Silvia G. Chuartzman; Bruce Morgan; Uri Weill; Eden Yifrach; Tobias P. Dick; Sara J. Cooper; Christer S. Ejsing; Maya Schuldiner; Einat Zalckvar; Sven Thoms ABSTRACT Peroxisomes are cellular organelles with vital functions in lipid, amino acid and redox metabolism...

...Alexander Aranovich; Rong Hua; Andrew D. Rutenberg; Peter K. Kim ABSTRACT The endoplasmic reticulum (ER) is required for the de novo biogenesis of peroxisomes in mammalian cells. However, its role in peroxisome maintenance is unclear. To explore ER involvement in the maintenance of peroxisomes, we...

... of substrates to nascent autophagosomes, their precise roles are not well understood. We examined selective autophagic degradation of peroxisomes (pexophagy), and found that NBR1 is necessary and sufficient for pexophagy. Mutagenesis studies of NBR1 showed that the amphipathic α-helical J domain, the ubiquitin...

...Hannah K. Delille; Brigitte Agricola; Sofia C. Guimaraes; Heike Borta; Georg H. Lüers; Marc Fransen; Michael Schrader Peroxisomes are ubiquitous subcellular organelles, which multiply by growth and division but can also form de novo via the endoplasmic reticulum. Growth and division of peroxisomes...

..., proteins of the membrane fission machinery (Dnm1-Mdv1-Caf4-Fis1 in yeast and DLP1-FIS1 in human) have been shown to have a dual localization on mitochondria and peroxisomes, where they control mitochondrial fission and peroxisome division. Here, we show that whereas vacuole fusion is regulated...

...Adabella van der Zand; Ineke Braakman; Hans J. Geuze; Henk F. Tabak Of the classical compartments of eukaryotic cells, peroxisomes were the last to be discovered. They are small, single-membrane-bound vesicles involved in cellular metabolism, most notably the β-oxidation of fatty acids...

... studies revealed that HpPex20p is predominantly associated with peroxisomes. Using fluorescence correlation spectroscopy we determined the native molecular mass of purified HpPex20p and binding of a synthetic peptide containing a PTS2 sequence. The data revealed that purified HpPex20p forms oligomers...

...Vasily D. Antonenkov; Raija T. Sormunen; J. Kalervo Hiltunen The functional role of the peroxisomal membrane as a permeability barrier to metabolites has been a matter of controversy for more than four decades. The initial conception, claiming free permeability of the membrane to small solute...

...Carlo W. T. van Roermund; Mark de Jong; Lodewijk IJlst; Jan van Marle; Tobias B. Dansen; Ronald J. A. Wanders; Hans R. Waterham Peroxisomes have a central function in lipid metabolism, including the β-oxidation of various fatty acids. The products and substrates involved in the β-oxidation have...

...Sietske Hogenboom; John J. M. Tuyp; Marc Espeel; Janet Koster; Ronald J. A. Wanders; Hans R. Waterham In the past decade several reports have appeared which suggest that peroxisomes play a central role in isoprenoid/cholesterol biosynthesis. These suggestions were based primarily on the reported...

...Mary Q. Stewart; Renee D. Esposito; Jehangir Gowani; Joel M. Goodman Alcohol oxidase (AO) and dihydroxyacetone synthase (DHAS) constitute the bulk of matrix proteins in methylotrophic yeasts, model organisms for the study of peroxisomal assembly. Both are homooligomers; AO is a flavin-containing...

...Daniel L. Tuttle; William A. Dunn, Jr ABSTRACT The budding yeast Pichia pastoris responds to methanolic media by synthesizing high levels of cytosolic enzymes (e.g. formate dehydrogenase) and peroxisomal enzymes (e.g. alcohol oxidase), which are necessary to assimilate this carbon source. Major...

... that the chemiluminescence results from lipid peroxidation reactions following oxygen reduction by leakage of electrons from ubiquinone in mitochondrial electron transport, andperhaps by peroxisomal oxidation of urate. Our results are consistent with the view that heat shock causes oxidative stress, which in turn induces...

... of mammalian species (marmoset, baboon, cat, rabbit, pig, sheep) using the techniques of post-embedding protein A-gold immunoelectron microscopy and isopycnic density gradient centrifugation. In baboon and rabbit livers, AGT1 was exclusively peroxisomal. Morphometric analysis of the distribution...

...Joel M. Goodman; Steven B. Trapp; Harold Hwang; Marten Veenhuis ABSTRACT Peroxisomes massively proliferate in the methylotrophic yeast Candida boidinii when cultured on methanol as the only carbon and energy source. These organelles contain enzymes that catalyze the initial reactions of methanol...