...Justyna Bajdzienko; Anja Bremm ABSTRACT Peroxisomes are highly plastic organelles that are involved in several metabolic processes, including fatty acid oxidation, ether lipid synthesis and redox homeostasis. Their abundance and activity are dynamically regulated in response to nutrient...

... other functions. Peroxisomal membrane contact sites have obtained little attention compared to those of other cell organelles. However, recent studies resulted in a big leap in our knowledge of the occurrence, composition and function of peroxisomal contact sites. Studies in yeast strongly contributed...

...Lakhan Ekal; Abdulaziz M. S. Alqahtani; Ewald H. Hettema ABSTRACT Dynamin-related proteins (Drps) mediate a variety of membrane remodelling processes. The Saccharomyces cerevisiae Drp, Vps1, is required for endocytosis, endosomal sorting, vacuole fusion, and peroxisome fission and breakdown. How...

... Gama ABSTRACT Mitochondria and peroxisomes are dynamic signaling organelles that constantly undergo fission, driven by the large GTPase dynamin-related protein 1 (DRP1; encoded by DNM1L ). Patients with de novo heterozygous missense mutations in DNM1L present with encephalopathy due to defective...

...Yuichi Yagita; Yuichi Abe; Yukio Fujiki ABSTRACT Mammalian PEX16 has been considered essential for generating and maintaining peroxisomal membranes. This view is based primarily on the finding that fibroblasts from several PEX16 -deficient patients are devoid of peroxisomal structures but can form...

... and peroxisomes. During ERS responses, mitochondrial respiration increases to ameliorate reactive oxygen species (ROS) accumulation. We now show in yeast that peroxisome abundance also increases to promote an adaptive response. In pox1 Δ cells, which are defective in peroxisomal β-oxidation of fatty acids...

...Nicolò Ilacqua; Irene Anastasia; Andrea Raimondi; Philippe Lemieux; Thomas Q. de Aguiar Vallim; Katalin Toth; Eugene V. Koonin; Luca Pellegrini ABSTRACT Hepatic lipid homeostasis depends on intracellular pathways that respire fatty acid in peroxisomes and mitochondria, and on systemic pathways...

.... In the methylotrophic yeast Komagataella phaffii (Kp; also known as Pichia pastoris ), the plasma membrane protein KpWsc1 senses environmental methanol concentrations and transmits this information to induce the expression of genes for methanol metabolism and the formation of huge peroxisomes. In this study, we show...

... family proteins localize to the endoplasmic reticulum (ER). Pex24 and Pex32, but not Pex23 and Pex29, predominantly accumulate at peroxisome–ER contacts. Upon deletion of PEX24 or PEX32 – and to a much lesser extent, of PEX23 or PEX29 – peroxisome–ER contacts are lost, concomitant with defects...

...Yukio Fujiki; Yuichi Abe; Yuuta Imoto; Akemi J. Tanaka; Kanji Okumoto; Masanori Honsho; Shigehiko Tamura; Non Miyata; Toshihide Yamashita; Wendy K. Chung; Tsuneyoshi Kuroiwa ABSTRACT Peroxisomes are single-membrane organelles present in eukaryotes. The functional importance of peroxisomes in humans...

...Hideaki Tanaka; Tomohiko Okazaki; Saeko Aoyama; Mutsumi Yokota; Masato Koike; Yasushi Okada; Yukio Fujiki; Yukiko Gotoh ABSTRACT Peroxisomes cooperate with mitochondria in the performance of cellular metabolic functions, such as fatty acid oxidation and the maintenance of redox homeostasis. However...

... peroxisomal membrane protein that generates the fatty alcohols required for the biosynthesis of ether lipids. Lipid droplet localization of exogenously expressed and endogenous human Far1 was observed by fluorescence microscopy under conditions of increased triglyceride synthesis in tissue culture cells...

... that myogenic fetal cells have distinct metabolic requirements compared to those proliferating during regeneration, with the former displaying a low respiration demand relying mostly on glycolysis. Furthermore, we show distinct requirements for peroxisomal and mitochondrial fatty acid oxidation (FAO...

...Sanjeev Kumar Choudhry; Rinse de Boer; Ida J. van der Klei ABSTRACT Here, we used fluorescence microscopy and a peroxisome-targeted tandem fluorescent protein timer to determine the relative age of peroxisomes in yeast. Our data indicate that yeast cells contain a heterogeneous population...

...Francisco J. Corpas; Juan B. Barroso; Jenny Russinova ABSTRACT Ca 2+ is a second messenger in many physiological and phytopathological processes. Peroxisomes are subcellular compartments with an active oxidative and nitrosative metabolism. Previous studies have demonstrated that peroxisomal nitric...

...Francisco J. Corpas; Juan B. Barroso; Jenny Russinova ABSTRACT Plant peroxisomes are recognized organelles that – with their capacity to generate greater amounts of H 2 O 2 than other subcellular compartments – have a remarkable oxidative metabolism. However, over the last 15 years, new information...

... the division of mitochondrion, plastid and peroxisome. Here, using isolated peroxisome-dividing (POD) machinery from a unicellular red algae, Cyanidioschyzon merolae , we identified a dynamin-based ring organizing center (DOC) that acts as an initiation point for formation of the dynamin-based ring. C. merolae...

...Daniel Effelsberg; Luis Daniel Cruz-Zaragoza; Wolfgang Schliebs; Ralf Erdmann ABSTRACT Peroxisomal proteins carrying a type 1 peroxisomal targeting signal (PTS1) are recognized by the well-conserved cycling import receptor Pex5p. The yeast YMR018W gene encodes a Pex5p paralog and newly identified...

...Eden Yifrach; Silvia G. Chuartzman; Noa Dahan; Shiran Maskit; Lior Zada; Uri Weill; Ido Yofe; Tsviya Olender; Maya Schuldiner; Einat Zalckvar ABSTRACT To optimally perform the diversity of metabolic functions that occur within peroxisomes, cells must dynamically regulate peroxisome size, number...

...Jinlan Chang; Mary J. Klute; Robert J. Tower; Fred D. Mast; Joel B. Dacks; Richard A. Rachubinski ABSTRACT The peroxin Pex11 has a recognized role in peroxisome division. Pex11p remodels and elongates peroxisomal membranes prior to the recruitment of dynamin-related GTPases that act in membrane...