... pericentriolar material (PCM) protein for microtubule organization of the human centrosome. Here, we provide evidence that CEP215 exhibits a dynamically suppressed, solid-like state in interphase centrosomes, and becomes a more dynamic state in mitotic centrosomes. Specific interaction with PCNT, another...

... cilia of murine intramedullary collecting duct cells. The shortening of primary cilia caused by hyperosmotic shock followed delocalization of the pericentriolar material (PCM). Excessive microtubule and F-actin formation in the cytoplasm coincided with the hyperosmotic shock-induced changes to primary...

... multiple functions at centrioles and centrosomes, and we have previously shown that in Drosophila phosphorylated Sas-4 initiates Polo recruitment to newly formed centrioles, while phosphorylated Spd-2 recruits Polo to the pericentriolar material (PCM) that assembles around mother centrioles in mitosis...

...Jaeyoun Kim; Jeongjin Kim; Kunsoo Rhee ABSTRACT A centrosome consists of a pair of centrioles and pericentriolar material (PCM). We manipulated expression of PCNT, a key PCM protein, and investigated roles of PCM in centriole behavior during mitosis. Deletion of PCNT had little effect on interphase...

... of mitotic centrosomes.   J. Cell Biol.   135 , 1701 – 1713 . 10.1083/jcb.135.6.1701 Lawo   S. , Hasegan   M. , Gupta   G. D. , Pelletier   L. (2012). Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material.   Nat. Cell Biol.   14...

... of this environment with the formation of asymmetrically placed scaffolds onto which new basal bodies assemble and are positioned. Recent studies illuminate the positioning of nascent centrioles relative to a modular pericentriolar material (PCM) environment and suggest that, like ciliates, centrosomes organize...

...-expressing cells, suggesting that NEK6 and NEK7 might share biological activities that induce centriole duplication. The centrosomal pericentriolar material (PCM) proteins were significantly reduced in NEK7-depleted cells. The PCM proteins in NEK7-depleted cells did not accumulate at the centrosomes, even...

... centrosome replications in Chinese hamster ovary (CHO) cells induced by hydroxyurea (HU), which is known to uncouple the centrosome cycle from the cell cycle. Green fluorescent protein (GFP)-tagged centrin2 expressed in CHO cells labels both centrioles and the pericentriolar material (PCM). Counting...

... Pericentriolar material Nuclear import In animal cells, centrosome-nucleated microtubule arrays function in a wide variety of cellular processes including cell division and chromosome segregation, directed cell movement, and interphase cytoplasmic organization (for reviews see Mazia, 1987 ; Vorobjev...

... KCl-treated centrosomes (centrosomes stripped of their microtubule nucleating activity by 1 M KCl treatment) were incubated in interphasic extracts. Electron microscopy and immunostaining by anti--tubulin and 5051 human anti-centrosome antibodies revealed that pericentriolar material (PCM...

... pericentriolar material from which microtubules originate but not with the centrioles themselves. It is also clear that these antigens are highly conserved during evolution. * Author for correspondence 13 10 1988 23 1 1989 © 1989 by Company of Biologists 1989 monoclonal antibodies...

... hand, failed to show a multiplicity of spindle poles even at 3·3 μM . Each pole of a multipolar spindle was associated with pericentriolar material, as shown by staining with an autoimmune serum specific for pericentriolar material. The number of locations with free pericentriolar material capable...