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Keywords: Paxillin
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Journal Articles
J Cell Sci jcs.258769.
Published: 23 September 2021
... to be validated. Here we used Bimolecular Fluorescence Complementation (BiFC) assays in combination with a substrate trapping mutant of PTP1B to directly examine whether relevant phosphotyrosines on paxillin and FAK are substrates of the phosphatase in the context of cell-matrix adhesion sites. We find...
Journal Articles
In collection:
Adhesion
J Cell Sci (2021) 134 (6): jcs254359.
Published: 29 March 2021
..., β1 integrin and paxillin. We examined how these interactions enable vimentin to regulate cell migration on collagen. In fibroblasts, depletion of vimentin increased talin-dependent activation of β1 integrin by more than 2-fold. Loss of vimentin was associated with reduction of β1 integrin clustering...
Includes: Supplementary data
Journal Articles
In collection:
Adhesion
J Cell Sci (2020) 133 (5): jcs232405.
Published: 02 March 2020
... capacity. IL-4 further increased motility and matrix-degrading capacity in mature macrophages, with additional changes in migration machinery gene expression. Finally, siRNA-induced reductions in the expression of the core adhesion proteins paxillin and leupaxin decreased macrophage spreading...
Journal Articles
J Cell Sci (2019) 132 (8): jcs224493.
Published: 25 April 2019
... matrix assembly. While the α integrin subunit determines extracellular ligand specificity, the β integrin chain binds to an acidic residue of the ligand, and cytoplasmic adapter protein families such as talins, kindlins and paxillin, to form mechanosensing cell matrix adhesions. Alternative splicing...
Includes: Supplementary data
Journal Articles
J Cell Sci (2017) 130 (21): 3764–3775.
Published: 01 November 2017
..., the mechanistic details remain unclear. Here, we show that kindlins interacted directly with paxillin and that this interaction could support integrin αIIbβ3 activation. An exposed loop in the N-terminal F 0 subdomain of kindlins was involved in mediating the interaction. Disruption of kindlin binding to paxillin...
Includes: Supplementary data
Journal Articles
In collection:
Adhesion
J Cell Sci (2017) 130 (9): 1612–1624.
Published: 01 May 2017
... (FLAP) experiments, we found that the mobility of core FA proteins correlates with their function. Structural proteins such as tensin, talin and vinculin are significantly less mobile in FAs than signaling proteins such as FAK (also known as PTK2) and paxillin. The mobilities of the structural proteins...
Includes: Supplementary data
Journal Articles
J Cell Sci (2017) 130 (6): 1064–1077.
Published: 15 March 2017
... of either RhoG or SGEF is silenced, invadopodia are more stable and have a longer lifetime than in control cells. Our findings also demonstrate that RhoG and SGEF modulate the phosphorylation of paxillin, which plays a key role during invadopodia disassembly. In summary, we have identified a novel signaling...
Includes: Supplementary data
Journal Articles
In collection:
Adhesion
J Cell Sci (2016) 129 (10): 1981–1988.
Published: 15 May 2016
...Aneesh R. Sathe; G. V. Shivashankar; Michael P. Sheetz ABSTRACT The nuclear transport of paxillin appears to be crucial for paxillin function but the mechanism of transport remains unclear. Here, we show that the nuclear transport of paxillin is regulated by focal adhesion turnover and the presence...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (16): 3106–3116.
Published: 15 August 2015
...Pavel Marášek; Rastislav Dzijak; Irina Studenyak; Jindřiška Fišerová; Lívia Uličná; Petr Novák; Pavel Hozák ABSTRACT Paxillin (PXN) is a focal adhesion protein that has been implicated in signal transduction from the extracellular matrix. Recently, it has been shown to shuttle between the cytoplasm...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (18): 3928–3942.
Published: 15 September 2014
... the actin cytoskeleton to the extracellular matrix. Dynamic turnover of focal adhesions is crucial for cell migration. Paxillin is a multi-adaptor protein that plays an important role in regulating focal adhesion dynamics. Here, we identify TRIM15, a member of the tripartite motif protein family...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (8): 1672–1683.
Published: 15 April 2014
... cell migration required for cancer microvessel formation. Recently, it has been shown that the focal adhesion protein paxillin is required for directional migration of fibroblasts in vitro . Here, we show that paxillin knockdown enhances endothelial cell migration in vitro and stimulates angiogenesis...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (11): 2381–2391.
Published: 01 June 2013
... in several NSCLC cell lines as well as in HeLa cells. One known substrate for ERK kinases in cell migration, the focal adhesion protein paxillin, was also one of the hits identified in the screen. We demonstrate that HGF stimulation results in a time-dependent phosphorylation of paxillin on serine 126...
Includes: Supplementary data
Journal Articles
J Cell Sci (2012) 125 (16): 3733–3738.
Published: 15 August 2012
...Carine Rosse; Katrina Boeckeler; Mark Linch; Simone Radtke; David Frith; Karin Barnouin; Ali Sayed Morsi; Majid Hafezparast; Michael Howell; Peter J. Parker Summary In migrating NRK cells, aPKCs control the dynamics of turnover of paxillin-containing focal adhesions (FA) determining migration rate...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2011) 124 (8): 1316–1327.
Published: 15 April 2011
... interactions that are essential for the assembly of this ILK-containing complex at Drosophila muscle attachment sites (MASs). We show that, downstream of integrins, talin plays a decisive role in the recruitment of three proteins: ILK, PINCH and paxillin. The accumulation of ILK at MASs appears to follow...
Includes: Supplementary data
Journal Articles
J Cell Sci (2010) 123 (16): 2725–2732.
Published: 15 August 2010
... with a chemiluminescent detection system (ECL: Amersham). Primary antibodies were obtained from Santa Cruz (paxillin), Cell Signaling (ERK1/2- P ) or Abcam (paxillin Ser126- P ). The anti-GFP and anti-FLAG monoclonal antibodies were provided by Cancer Research UK, Research Services. For the detection of ERK1/2...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2010) 123 (9): 1567–1577.
Published: 01 May 2010
...Larisa Y. Romanova; Gibran Holmes; Svenja K. Bahte; Alexander L. Kovalchuk; Patrick J. Nelson; Yvona Ward; Faikah Gueler; J. Frederic Mushinski We investigated the PKCδ-mediated phosphorylation of paxillin within its LIM4 domain and the involvement of this phosphorylation in activation of LFA-1...
Includes: Supplementary data
Journal Articles
J Cell Sci (2009) 122 (12): 2127–2136.
Published: 15 June 2009
... adhesion, cell spreading and endocytosis. However, in contrast to Rac1, the Rac3-GIT1 interaction is not mediated by βPix. Interestingly, Rac3 expression severely attenuates the interaction between GIT1 and paxillin, accompanied by defective paxillin distribution, focal adhesion formation and disturbed...
Journal Articles
J Cell Sci (2009) 122 (10): 1654–1664.
Published: 15 May 2009
.... The pro-migratory activity of α4 integrin is mediated in part through the direct binding of the cytoplasmic domain to paxillin. Here, using intermolecular FRET and biochemical analyses, we report a novel interaction of the α4 integrin cytoplasmic domain with 14-3-3ζ. This interaction depends on serine...
Includes: Supplementary data
Journal Articles
J Cell Sci (2008) 121 (6): 854–864.
Published: 15 March 2008
... could be rescued by the expression of wild-type IKAP but not by FD-IKAP. No downregulation in the protein levels of paxillin or beclin 1, which were recently described as specific transcriptional targets of IKAP, was detected. These results provide evidence for the role of the cytosolic interactions...
Includes: Supplementary data
Journal Articles
J Cell Sci (2008) 121 (2): 196–204.
Published: 15 January 2008
... higher at adhesions than elsewhere, indicative of a site-specific biochemical trigger. The observed catastrophe induction occurred at adhesion domains containing the scaffolding protein paxillin that has been shown previously to interact with tubulin. Furthermore, replacement of full-length paxillin...
Includes: Multimedia, Supplementary data