...Megha Agarwal; Anushree Bharadwaj; Sam J. Mathew ABSTRACT Muscle stem (satellite) cells express Pax7, a key transcription factor essential for satellite cell maintenance and adult muscle regeneration. We identify the corepressor transducin-like enhancer of split-4 (TLE4) as a Pax7 interaction...

... has bimodal effects, inhibiting myogenic differentiation and blocking MyoD at low levels of expression, and killing myoblasts at high levels. Pax3 and Pax7, which contain related homeodomains, antagonize the cell death phenotype of DUX4 in C2C12 cells, suggesting some type of competitive interaction...

...Francesca Riuzzi; Guglielmo Sorci; Roberta Sagheddu; Angelo Sidoni; Rita Alaggio; Vito Ninfo; Rosario Donato ABSTRACT Embryonal rhabdomyosarcomas (ERMSs) show elevated levels of PAX7, a transcription factor that marks quiescent adult muscle stem (satellite) cells and is important for proliferation...

... cell pool. The most lateral fraction of the fast domain in the myotome of zebrafish embryos derives from the Pax7-positive dermomyotome-like cells. In this study, we show that two genes, belonging to the sine oculus class 1 ( six1 ) genes ( six1a and six1b ), are both essential for the regulation...

... capacity is not altered. By contrast, Pax7 haploinsufficiency does not show major modifications by transcriptome analysis. These observations provide a mechanism linking Myf5 levels to muscle stem cell heterogeneity and fate by exposing two distinct and opposing phenotypes associated with Myf5...

...Francesca Riuzzi; Guglielmo Sorci; Roberta Sagheddu; Rosario Donato Expression of the paired-box 7 (PAX7) transcription factor is regulated during both myoblast proliferation and differentiation: high levels of PAX7 compromise myogenic differentiation because of excess and prolonged proliferation...

... for correspondence ( amy.wagers@joslin.harvard.edu ) 30 4 2010 © 2010. 2010 Pax3 Pax7 Muscle stem cells Satellite cells C2C12 Myogenic differentiation Myogenic regulatory factors The PAX family consists of nine transcription factors, which have common paired, homeodomain...

... ) ‡ These authors contributed equally 20 9 2009 © The Company of Biologists Limited 2009 2009 Satellite cell Myoblast Presenilin-1 Id1 Pax7 MyoD γ-secretase Self-renewal Skeletal muscle Myogenic differentiation Stem cell Cell fate choice Muscle satellite cells are myogenic...

... in vitro or C2C12 myogenic cells, we found that Notch activity is essential for maintaining the expression of Pax7, a transcription factor associated with the self-renewal lineage, in quiescent undifferentiated myoblasts after they exit the cell cycle. Stimulation of the Notch pathway by expression...

... Pax7 without any MyoD – therefore, adopting the self-renewal pathway, with fewer cells undergoing myogenic differentiation. Similarly, preventing the degradation of endogenous β-catenin by inhibiting GSK3β activity also results in more Pax7-positive–MyoD-negative (Pax7 + MyoD – ) satellite-cell progeny...

... expression of Lbx1 in satellite-cell-derived C2C12 myoblast cells resulted in severe depression of myogenic differentiation and incomplete myotube formation, concomitantly with the activation of the paired-box transcription factor Pax7 and depression of the myogenic regulatory factor MyoD. Moreover...

... satellite cells express the transcription factor Pax7 and when activated, coexpress Pax7 with MyoD. Most then proliferate, downregulate Pax7 and differentiate. By contrast, others maintain Pax7 but lose MyoD and return to a state resembling quiescence. Here we show that Pax7 is able to drive transcription...