... assembly in human cells. To ascertain its role in ciliogenesis, we have investigated the function of its ortholog, PtFOR20p, in the multiciliated unicellular organism Paramecium . Using combined functional and cytological analyses, we found that PtFOR20p specifically localises at basal bodies...

... in Paramecium , which possesses an extended contractile array – the infraciliary lattice (ICL) – essentially composed of centrins and a huge Sfi1p-like protein, PtCenBP1p, which is essential for ICL assembly and contractility. The high molecular diversity revealed here by the proteomic analysis of the ICL...

...Ivonne M. Sehring; Christoph Reiner; Jörg Mansfeld; Helmut Plattner; Roland Kissmehl To localize the different actin paralogs found in Paramecium and to disclose functional implications, we used overexpression of GFP-fusion proteins and antibody labeling, as well as gene silencing. Several...

...Eva-Maria Ladenburger; Iris Korn; Nicole Kasielke; Thomas Wassmer; Helmut Plattner In the ciliate Paramecium , a variety of well characterized processes are regulated by Ca 2+ , e.g. exocytosis, endocytosis and ciliary beat. Therefore, among protozoa, Paramecium is considered a model organism...

...Roland Kissmehl; Marine Froissard; Helmut Plattner; Massoud Momayezi; Jean Cohen N-ethylmaleimide (NEM)-sensitive factor (NSF), a regulator of soluble NSF attachment protein receptors (SNAREs), is required for vesicular transport in many eukaryotic cells. In the ciliated protozoon Paramecium...

...Kwanghee Kim; Min Son; Joan B. Peterson; David L. Nelson We purified two small, acidic calcium-binding proteins( P aramecium C a 2+ - b inding p roteins, PCBP-25α and PCBP-25β) from Paramecium tetraurelia by Ca 2+ -dependent chromatography on phenyl-Sepharose and by anion-exchange chromatography...

...Takashi Tominaga; Yutaka Naitoh; Richard D. Allen ABSTRACT The contractile vacuole complex of the fresh water protozoan Paramecium multimicronucleatum exhibits periodic exocytotic activity. This keeps cytosolic osmolarity at a constant value. The contractile vacuole, the central exocytotic vesicle...

...Kenji Hasegawa; Hiroto Kikuchi; Shigeo Ishizaki; Akiko Tamura; Yasuo Tsukahara; Yasuo Nakaoka; Eiichi Iwai; Toshiro Sato ABSTRACT The circadian dynamics of cyclic adenosine 3′,5′-monophosphate (cAMP) and cyclic guanosine 3′,5′-monophosphate (cGMP) were simulated in Paramecium multimicronucleatum...

...W. Q. Yang; C. Braun; H. Plattner; J. Purvee; J. L. Van Houten ABSTRACT Glutamate is an attractant stimulus to Paramecium tetraurelia . It causes a hyperpolarization of the cell and smooth, relatively fast swimming that is characteristic of hyperpolarizing stimuli. We show here that by 1-30 seconds...

...Norbert Klauke; Helmut Plattner ABSTRACT In Paramecium tetraurelia cells analysis of transient changes in Ca 2+ concentration, [Ca 2+ ] i , during aminoethyldextran (AED) stimulated synchronous (<1 second ) trichocyst exocytosis has been hampered by various technical problems which we now have...

...Malgorzata Prajer; Anne Fleury; Michel Laurent ABSTRACT This paper is the first report of the use of a fluorescent indicator (Dextran-coupled calcium green-1) for imaging of cytosolic free calcium in ciliate cells. Using this technique in Paramecium , we show that a very transient increase...

...Nobuyuki Haga ABSTRACT The expression of sexual activity in Paramecium caudatum is repressed for about 50 fissions after conjugation. The ability of the macronucleus to affect the expression of sexual activity according to the age of clonal development was investigated using the macronuclear...

...Agnes K. Fok; Marilynn S. Aihara; Masaki Ishida; Kathleen V. Nolta; Theodore L. Steck; Richard D. Allen ABSTRACT Our previous study has shown that the decorated tubules (collectively known as the decorated spongiome) of the contractile vacuole complex (CVC) in Paramecium are the site of fluid...

...Nicolette Levilliers; Anne Fleury; Anne-Marie Hill ABSTRACT Polyclonal (PAT) and monoclonal (AXO 49) antibodies against Paramecium axonemal tubulin were used as probes to reveal tubulin heterogeneity. The location, the nature and the subcellular distribution of the epitopes recognized...

.... * Author for correspondence 12 08 1994 20 02 1995 © 1995 by Company of Biologists 1995 calcium SIMS Paramecium The question of the subcellular location of calcium (Ca) stores in eukaryotic cells has attracted considerable attention in recent years (see Koch, 1990...

... the dynamics of membrane retrieval in the phagosome system of Paramecium multimicronucleatum using six monoclonal antibody markers. Our previous freeze-fracture and transmission electron microscopic studies have indicated that extensive changes take place in the membrane of the young phagosome as it progresses...

...David J. Asai; Susan M. Beckwith; Kimberly A. Kandl; Heather H. Keating; Hendri Tjandra; James D. Forney ABSTRACT Paramecium tetraurelia is a unicellular organism that utilizes both axonemal and cytoplasmic dyneins. The highly conserved region containing the catalytic P-loop of the dynein heavy...

..., University of Hawaii, Honolulu, HI 96822, USA 07 07 1993 02 09 1993 © 1993 by Company of Biologists 1993 V-ATPase proton pump contractile vacuole complex calmodulin Dictyostelium monoclonal antibody osmoregulation Paramecium Our two laboratories are studying...

...Masaki Ishida; Marilynn S. Aihara; Richard D. Allen; Agnes K. Fok ABSTRACT In a previous study, monoclonal antibody DS-1 was found to specifically label the decorated spongiome along the radial arms of the contractile vacuole complexes in Paramecium multimicronucleatum . Fluorescein isothiocyanate...

...Richard D. Allen; Le Ma; Agnes K. Fok ABSTRACT Acidosomes are organelles that in Paramecium are responsible for the acidification of phagosomes before phagosomes fuse with lysosomes. Using a combination of (a) the quick-freeze deep-etch (QF-DE) technique, (b) monoclonal antibodies (mAbs) that label...